Biomarkers of Oxidative Stress in the Muscle Tissue of Rainbow Trout (Oncorhynchus Mykiss Walbaum) After in Vitro Treatment by Extracts Derived From Stalks and Roots of Greater Celandine (Chelidonium Majus L.)

Abstract

Consistent with our previous studies, we continue to evaluate the antioxidant potential of Greater celandine (Chelidonium majus L), a representative of the Papaveraceae family, collected from northern Poland using the model of muscle tissue of rainbow trout (Oncorhynchus mykiss Walbaum). Therefore, in the present study, oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), protein oxidative modification carbonyl derivative content, total antioxidant capacity (TAC)] were used to evaluate the antioxidant activity of extracts (final concentration 5 mg/mL) derived from stems and roots of C. majus. Rainbow trout muscle tissue was used in this study. Phosphate buffer was used as a positive control (blank). The results of the current study showed that stem and root extracts exhibited cytotoxic effects on cellular structures of muscle tissue by increasing the level of the lipid peroxidation biomarkers. These results suggest the possibility of using C. majus extract at 5 mg/mL as a source of pro-oxidant compounds and warrant further studies to evaluate their therapeutic potential. Levels of aldehydic and ketonic derivatives of oxidatively modified proteins and total antioxidant capacity were not significantly changed after in vitro incubation with the extracts derived from stalks and roots of C. majus. Screening of species of the family Papaveraceae for other biological activities, including antioxidant activity, is essential and may be effective in the search for preventive measures in the pathogenesis of some diseases, as well as in the prevention and treatment of some disorders in medicine and veterinary.