Effects of extraction methods on structural characteristics and bile acid‐binding capacities of Moringa oleifera leaf polysaccharide fractions

Abstract

Summary There is an urgent need for safe and effective bile acid sequestrants from edible plants to treat hyperlipidaemia. Moringa oleifera leaf has been reported to be a source of hypolipidaemic agents. However, limited information is available on the bile acid‐binding capacity of M. oleifera leaf polysaccharide fraction (MOLPF). To illustrate structure‐bile acid‐binding capacity relationship of MOLPFs obtained by eight common extraction methods and provide an extraction technique for MOLPF with strong bile acid‐binding capacity, structure characteristics and bile acid‐binding capacities of MOLPFs were investigated. The results indicated that extraction method greatly affected morphology, monosaccharide composition, glycosidic linkage and molecular weight distribution of MOLPF. MOLPFs with lower contents of galactose and more branched components exhibited lower binding rates. MOLPFs prepared by cellulase and ultrasonic‐assisted cellulase extraction methods possessing the highest contents of galactose and relatively high proportions of linear macromolecules exhibited the strongest binding capacities, showing potential hypolipidaemic effects.

Optimization Ultrasound-Assisted Deep Eutectic Solvent Extraction of Anthocyanins from Raspberry Using Response Surface Methodology Coupled with Genetic Algorithm

“…In addition, the low temperature and the short extraction time of UAE can avoid the degradation of anthocyanins caused by high temperature, which contributes to the improvement of the anthocyanin yield [ 10 ]. Thus, the UAE method is used in the extraction of bioactive compounds from different natural plant sources [ 11 , 12 , 13 ]. The combination of UAE and DESs extraction methods has the advantages of both methods, thereby providing effective and environmentally friendly alternatives to the traditional anthocyanin extraction methods.…”

Interaction of the Exopolysaccharide from Lactobacillus plantarum YW11 with Casein and Bioactivities of the Polymer Complex

“…Previously, polysaccharides extracted from loquat leaves exerted remarkable in vitro binding capacities for bile acids [ 64 ]. Moringa oleifera leaf polysaccharide fraction with the highest galactose content and a large proportion of linear macromolecules exhibited the strongest bile acid binding capacity with potential hypolipidemic effects [ 65 ].…”

Recent developments in Moringa oleifera Lam. polysaccharides: A review of the relationship between extraction methods, structural characteristics and functional activities

“…Macroporous resin could separate polysaccharides with different ionic strengths. AB-8 and HP-20 showed better decolorization of MOPs ( Yang, Zhao, & Lin, 2020 ). Macroporous resin has good decolorization effect and polysaccharide retain high activity after decolorization.…”

“… HPGPC, FT-IR, SEM Rheometer 4.86 × 10 3 Xyl, Man, Glu, Gala was 62.55%,8.16%,19.91%,4.74% ( Chen et al, 2017 ) Leaves Water (55℃, 2 h), Ultrasound power (800 W) NS37071 enzyme 1% EtoH (4℃, 12 h), Sevag method. SEC , HPLC, GC/MS, ACQUITY HPLC ＞10 3 Man, Rha, Glc, Gal, Ara GlcA, GalA was 5.66%,73.8%,28.17%,32.37%20.04%,1.20%,5.18% ( Yang et al, 2020 ) Leaves Water (100℃, 2 h) EtoH (4℃, 24 h), sevag method, DEAE Sepharose Fast flow ion exchang colum (2.6 cm × 10 cm), NaCl (0.5 mol/L), 500–1000 Da Membranes. GPC, FT-IR, SEM, UV, AFM, Methylation 104 × 10 3 β-Pyr and Man ( Tian et al, 2021 ) Leaves Water (90℃, 4 h) EtoH (4℃, 24 h), sevag method, DEAE Sepharose Fast flow ion exchange column (1.6 cm × 20 cm), 0.2 mol/L NaCl, 500–1000 Da membranes HPGPC, UV, Congo red method, GC–MS, 1 H and 13 C NMR, Methylation 4.033 × 10 3 Ara: Glu: Gla was 47.73:1.00:57.65 ( Li et al, 2020 ) Leaves Water (90℃, 4 h) EtoH (4℃, 12 h), sevag method, D 101, DEAE Sepharose Fast flow ion exchange column (1.6 cm × 20 cm), 0.12 mol/L NaCl 3000 Da membranes HPGPC, GC–MS, Methylation, NMR 155.35 Ara: Glu: Gala was 35.8%, 6.67% and 57.53% ( Dong et al, 2018 ) Leaves Water (90℃, 4 h) Agilent Technologies HPLC, zorbax SB-C18 (250 × 4.6 mm, 5 mm), 5% acetonitrile HPLC-DAD, HPLC-RID NA Raf, Sta, Gra, Xyl, Man, Ara ( Caicedo-Lopez, Luzardo-Ocampo, Cuellar-Nunez, Campos-Vega, Mendoza, & Loarca-Pina, 2019 ) Flowers Water (Ice bath 4 h) Ultrasonic (800 W) EtoH (4℃, 12 h), sevag method, D101, DEAE-52 (2.6 cm × 60 cm), NaCl (0–2 mol/L), DEAE Sepharose 6B (2 × 200 cm), 0.1 mol/L NaCl, 3500 Da membranes.…”

“…A special α-Pyranoside bond occurs at 845 cm −1 , and β-Pyranoside bond occurs at 890 cm −1 . Pyranoside occurs in three absorption peaks between 1100 cm −1 and 1010 cm −1 , while the acetyl group on the glucoside could appear at 1730 cm −1 ( Yang et al, 2020 ).…”