Effects of extracellular products of
            <b>
              <i>Vibrio alginolyticus</i>
            </b>
            on penaeid prawn plasma components

Lee, K.‐K.; Chen, F.‐R.; Yu, Shu-Ru; Yang, Tun-I; Liu, P.‐C.

doi:10.1046/j.1472-765x.1997.00175.x

Cited by 15 publications

(19 citation statements)

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“…Although the virulence factors of V. alginolyticus have not been identified, Lee et al. (1997a,b) suggested that alkaline serine protease and other extracellular products may be involved in the virulence. Few representative isolates (n = 6) tested for virulence in the present study showed an LD 50 of >10 7 cells ml –1 for P. monodon postlarvae (PL 20), indicating their low virulence.…”

Section: Discussionmentioning

confidence: 99%

Bacteriological study of shrimp, Penaeus monodon Fabricius, hatcheries in India

Otta¹,

Karunasagar²

2001

J Appl Ichthyol

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Shrimp hatcheries often face problems of mortality caused by diseases. To understand the bacteriological status of shrimp, Penaeus monodon Fabricius, hatcheries in India, a study of hatchery water at different points was conducted in several hatcheries located along the east and west coast of India. The species composition of the bacterial flora was also determined. The total plate counts of raw sea water on tryptic soya agar ranged from 102 to 104 ml–1, whereas it ranged from 104 to 106 ml–1 in larval tanks. In the larval tanks, the proportion of Vibrio species ranged from 50% to 73%, as compared to 31% in raw sea water. A mixed bacterial flora was observed in hatchery water; however, in the larval tanks, the flora in the larvae was predominantly made up of Vibrio species. A few of the tested Vibrio isolates were non‐virulent to shrimp larvae under experimental conditions. Over 90% of the strains were resistant to amoxycillin, ampicillin, cephalexin, cephazolin, cloxacillin and sulphafurazole. Most strains showed sensitivity to tetracycline, chloramphenicol, and quinolones such as norfloxacin and ciprofloxacin.

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Section: Discussionmentioning

confidence: 99%

Bacteriological study of shrimp, Penaeus monodon Fabricius, hatcheries in India

Otta¹,

Karunasagar²

2001

J Appl Ichthyol

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“…For standardization, protease K (Sigma-Aldrich) was used. When indicated the metalloprotease inhibitor EDTA (5 mM) and the serine protease inhibitor phenylmethylsulfonyl fluoride (PMSF) (1 mM) were added prior to incubation [47].…”

Section: Methodsmentioning

confidence: 99%

Autoinducers Act as Biological Timers in Vibrio harveyi

Anetzberger

Reiger

Fekete³

et al. 2012

PLoS ONE

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Quorum sensing regulates cell density-dependent phenotypes and involves the synthesis, excretion and detection of so-called autoinducers. Vibrio harveyi strain ATCC BAA-1116 (recently reclassified as Vibrio campbellii), one of the best-characterized model organisms for the study of quorum sensing, produces and responds to three autoinducers. HAI-1, AI-2 and CAI-1 are recognized by different receptors, but all information is channeled into the same signaling cascade, which controls a specific set of genes. Here we examine temporal variations of availability and concentration of the three autoinducers in V. harveyi, and monitor the phenotypes they regulate, from the early exponential to the stationary growth phase in liquid culture. Specifically, the exponential growth phase is characterized by an increase in AI-2 and the induction of bioluminescence, while HAI-1 and CAI-1 are undetectable prior to the late exponential growth phase. CAI-1 activity reaches its maximum upon entry into stationary phase, while molar concentrations of AI-2 and HAI-1 become approximately equal. Similarly, autoinducer-dependent exoproteolytic activity increases at the transition into stationary phase. These findings are reflected in temporal alterations in expression of the luxR gene that encodes the master regulator LuxR, and of four autoinducer-regulated genes during growth. Moreover, in vitro phosphorylation assays reveal a tight correlation between the HAI-1/AI-2 ratio as input and levels of receptor-mediated phosphorylation of LuxU as output. Our study supports a model in which the combinations of autoinducers available, rather than cell density per se, determine the timing of various processes in V. harveyi populations.

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“…Studies have sought to explain the patho‐genicity mechanism of V. harveyi and to develop effective disease control strategies ( Itami, Takahashi & Nakamura 1989; Itami, Takahashi, Yoneoka & Yan 1991; Sung, Song & Kou 1991; Sung & Song 1996; Lee, Chen, Yu, Yang & Liu 1997a; Song, Liu, Chan & Sung 1997). Virulence is reported to be associated with the presence of extracell‐ular products (ECPs) ( Liu et al .…”

Section: Introductionmentioning

confidence: 99%

“…Virulence is reported to be associated with the presence of extracell‐ular products (ECPs) ( Liu et al . 1996a,b ), includ‐ing proteases, haemolysins and cytotoxins ( Lee et al . 1997a ; Liu et al .…”

Section: Introductionmentioning

confidence: 99%

Characterization of extracellular products from an isolate of Vibrio harveyi recovered from diseased post‐larval Penaeus vannamei (Bonne)

Montero

Austin

1999

Journal of Fish Diseases

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Vibrio harveyi recovered from diseased post‐larval Penaeus vannamei produced a thermostable exotoxin, which was lethal to Dublin Bay prawns, Nephrops norvegicus L., when injected intramuscularly. The extracellular products (ECPs) concentrated from tryptone soya broth supplemented with 1% (w/v) sodium chloride or from cellophane overlays on marine 2216E agar with incubation at 15, 22 and 27 °C were toxic, with the lethal dose 50% of the crude ECPs estimated to be 4.4 μgprotein prawn−1. Proteolytic, haemolytic and cytotoxic activities were detected, although the occurrence and quantity of these activities were influenced by cultural conditions. The ECPs which had been heated (100 °C for 10 min) or digested with protease K produced the same pathology as crude, untreated ECPs. Western blotting demonstrated that all the ECP preparations contained low molecular weight lipopolysaccharides, which may constitute the lethal toxin of V. harveyi.

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Effects of extracellular products of Vibrio alginolyticus on penaeid prawn plasma components

Cited by 15 publications

References 0 publications

Bacteriological study of shrimp, Penaeus monodon Fabricius, hatcheries in India

Bacteriological study of shrimp, Penaeus monodon Fabricius, hatcheries in India

Autoinducers Act as Biological Timers in Vibrio harveyi

Characterization of extracellular products from an isolate of Vibrio harveyi recovered from diseased post‐larval Penaeus vannamei (Bonne)

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