Characterization of extracellular products from an isolate of <i>Vibrio harveyi</i> recovered from diseased post‐larval <i>Penaeus vannamei</i> (Bonne)

Montero, Angel; Austin, Brian

doi:10.1046/j.1365-2761.1999.00189.x

Cited by 54 publications

(43 citation statements)

References 32 publications

(46 reference statements)

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“…Several isolates studied here showed moderate to strong activity for proteases and phospholipases, and these activities gave significant positive correlation with naupliar mortality. Other authors have also observed high enzyme activity in virulent Vibrio harveyi strains isolated from mollusks (Borrego et al 1996) and from shrimps and fishes (Liu et al 1996b, Montero & Austin 1999, Zhang & Austin 2000.…”

Section: Discussionmentioning

confidence: 85%

Virulence of luminous vibrios to Artemia franciscana nauplii

Soto-Rodríguez¹,

Roque²,

Lizárraga-Partida³

et al. 2003

Dis. Aquat. Org.

107

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From healthy and diseased penaeid shrimp from Asia and the Americas, 25 luminous and 2 non-luminous bacterial strains were isolated, and 14 were phenotypically identified as Vibrio harveyi; 9 isolates produced significant mortalities (45 to 80%) in Artemia franciscana nauplii at inoculation densities of 10 5 to 10 6 CFU ml -1 compared to the controls (unchallenged nauplii). The maximum number of bacteria ingested (bioencapsulated) by the Artemia nauplii varied from less than 10 to 10 3 CFU nauplius -1 and no significant relationship was observed between the density of bacteria inoculated, the amount of bacteria ingested, and naupliar mortality. Significant correlations were obtained between naupliar mortality and production of proteases, phospholipases or siderophores, but not between mortality and lipase production, gelatinase production, hydrophobicity or hemolytic activity. The results suggest that virulence of the strains tested was more related to the production of particular exoenzymes than to the measured colonization factors.

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Section: Discussionmentioning

confidence: 85%

Virulence of luminous vibrios to Artemia franciscana nauplii

Soto-Rodríguez¹,

Roque²,

Lizárraga-Partida³

et al. 2003

Dis. Aquat. Org.

107

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“…The putative toxic virulence factors contained in the PFs40 and the FPLC fraction from Vibrio penaeicida Strain AM101 were subjected to different neutralizing treatments before being injected intra-muscularly into shrimp (25 µl ind. ), aprotinin (4 µg ml -1 for 1 h at 37°C), CuCl 2 (5 mM for 30 min at 25°C), and trans-epoxysuccinyl-L-leucylamido (4-guanidino) butane or E-64 (0.5 mg ml -1 for 1 h at 37°C) , Montero & Austin 1999. Control shrimp groups were injected with one of the chemical inhibitors alone at the same concentrations.…”

Section: Methodsmentioning

confidence: 99%

“…show great variation in terms of pathogenicity associated with host species, developmental stage, bacterial dose, bacteria species and particular strains, and exposure time and stress (Lightner 1996, Saulnier et al 2000a, Aguirre-Guzmán et al 2001. Different Vibrio extracellular products (ECP) have been identified and proposed as putative virulence factors in the species pathogenic to shrimp (Liu et al 1996, 1999a,b, Chen et al 1999, Harris & Owens 1999, Montero & Austin 1999. A thermo-labile cytotoxic factor was detected in the ECP from V. penaeicida, which produces 100% mortality in juvenile Litopenaeus stylirostris (Goarant et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity of Vibrio penaeicida for white shrimp Litopenaeus vannamei: a cysteine protease-like exotoxin as a virulence factor

Aguirre‐Guzmán¹,

Ascencio²,

Saulnier³

2005

Dis. Aquat. Org.

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The pathogenicity of Vibrio penaeicida Strains KH-1 and AM101, their culture-free supernatant (CFS), and their protein fraction obtained by 40% of ammonium sulfate precipitation (PFs40) were assessed in experimental challenges against juvenile Litopenaeus vannamei. Live Vibrio cells, CFS, and PFs40 from the AM101 strain produced a significantly higher mortality (p < 0.05) compared to the KH-1 strain. Toxicity and median lethal doses (LD 50 ) of Fast Protein Liquid Chromatography (FPLC) products were evaluated on L. vannamei. The first FPLC fraction sample (A) from PFs40 of the AM101 strain displayed LD 50 values of 1.68 and 5.61 µg protein ind.-1 , respectively. The second FPLC process from Fraction A showed a peak (A1) also with toxic effects to shrimp. PFs40, Fraction A, and Peak A1 showed a 38.5 kDa molecular band (SDS-PAGE), with activity on a gelatin protease zymogram. The lethal effect of PFs40 and Fraction A was inhibited by Proteinase K, CuCl 2 , E-64, and heat (60 and 100°C) treatments, but was not inhibited by EDTA-Na 2 , aprotinin, and soy trypsin treatments. These results and the zymogram inhibition test suggest the presence of a cysteine protease-like proteinaceous exotoxin as a dominant protease, secreted by V. penaeicida Strain AM101. KEY WORDS: Vibrio penaeicida · Cysteine protease · Litopenaeus vannamei · Shrimp · Exotoxin · PathogenicityResale or republication not permitted without written consent of the publisher

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“…Several reports have shown that vibrio bacteria including V. alginolyticus, V. cholerae, V. parahaemolyticus, V. vulnificus, V. mimicus, and V. harveyi are the important pathogens, as these are always isolated from diseased L. vannamei (Lighter, 1993;Vandenberghe et al, 1999;Montero & Austin, 1999). Bacterial pathogens can cause problems that range from growth retardation to sporadic mortalities and mass mortalities of shrimp.…”

Section: Introductionmentioning

confidence: 99%

The Potential of Galangal (Alpinia galanga Linn.) Extract against the Pathogens that Cause White Feces Syndrome and Acute Hepatopancreatic Necrosis Disease (AHPND) in Pacific White Shrimp (Litopenaeus vannamei)

Chaweepack¹,

Muenthaisong²,

Chaweepack³

et al. 2015

IJB

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White feces syndrome and acute hepatopancreatic necrosis disease (AHPND) are serious diseases that have recently been noted in Pacific white shrimp (Litopenaeus vannamei). Vibrio bacteria and 6 species of fungi (Aspergillus flavus, A. ochraceus, A. japonicus, Penicillium sp., Fusarium sp., and Cladosporium cladosporioides) were isolated from shrimp naturally infected with white feces syndrome. Antibiotics have been used to treatment the disease for many years, but these have been ineffective and have resulted in drug residue problems for the shrimp industry. In this study, an alternative method was tested for its efficacy in controlling these pathogens. The crude extract of galangal (Alpinia galanga Linn.), an herbal medicine, inhibited the growth of 8 vibrio species of the pathogen, V. parahaemolyticus (EMS/AHPND) in particular. The results also showed that 0.5 mg/ml of the galangal extract was a concentration that produced the strongest inhibition of the fungi A. ochraceus. Naturally infested shrimp L. vannamei were fed 2 and 4% (v/w) portions of the herb extract for 12 days and their progress was compared with that of a control group (no herb extract). At the end of the feeding trial, the numbers of total Vibrio spp. and the incidence of fungi infestation in the hepatopancreas and intestines of treated shrimp were significantly lower than that in the control group (P<0.05). Furthermore, the survival rates for the treatment groups, after injections with V. parahaemolyticus (EMS/AHPND), were significantly higher than that of the control group (P<0.05). Based on these results, we can report that the galangal extract has antimicrobial properties that are applicable as bio-medicinal agents against white feces syndrome and AHPND. Therefore, in the future this herb should be an alternative to chemotherapeutic agents that are being used in the shrimp industry.

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Characterization of extracellular products from an isolate of Vibrio harveyi recovered from diseased post‐larval Penaeus vannamei (Bonne)

Cited by 54 publications

References 32 publications

Virulence of luminous vibrios to Artemia franciscana nauplii

Virulence of luminous vibrios to Artemia franciscana nauplii

Pathogenicity of Vibrio penaeicida for white shrimp Litopenaeus vannamei: a cysteine protease-like exotoxin as a virulence factor

The Potential of Galangal (Alpinia galanga Linn.) Extract against the Pathogens that Cause White Feces Syndrome and Acute Hepatopancreatic Necrosis Disease (AHPND) in Pacific White Shrimp (Litopenaeus vannamei)

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