Bacteriological study of shrimp, Penaeus monodon Fabricius, hatcheries in India

Abstract

Shrimp hatcheries often face problems of mortality caused by diseases. To understand the bacteriological status of shrimp, Penaeus monodon Fabricius, hatcheries in India, a study of hatchery water at different points was conducted in several hatcheries located along the east and west coast of India. The species composition of the bacterial flora was also determined. The total plate counts of raw sea water on tryptic soya agar ranged from 102 to 104 ml–1, whereas it ranged from 104 to 106 ml–1 in larval tanks. In the larval tanks, the proportion of Vibrio species ranged from 50% to 73%, as compared to 31% in raw sea water. A mixed bacterial flora was observed in hatchery water; however, in the larval tanks, the flora in the larvae was predominantly made up of Vibrio species. A few of the tested Vibrio isolates were non‐virulent to shrimp larvae under experimental conditions. Over 90% of the strains were resistant to amoxycillin, ampicillin, cephalexin, cephazolin, cloxacillin and sulphafurazole. Most strains showed sensitivity to tetracycline, chloramphenicol, and quinolones such as norfloxacin and ciprofloxacin.

Bacteria flora associated with different body parts of hatchery reared juvenilePenaeus monodon, tanks water and sediment

“…This condition provides good environment for higher bacteria production. Total plate count of larval and post larval tanks of several P. monodon hatcheries in India was reported in a range of 10 2 to 10 4 and 10 4 to 10 7 , respectively (Otta et al, 2001), There were no significant difference between rearing water of juvenile of black sea bream and red sea breams, the mean value were 3.1 x 10 4 (Muroga et al, 1987). The quantitative analysis of total bacteria of juvenile Penaeus japonicus rearing water was lower than 10 4 CFU/ml (Yasuda and Kiato, 1980).…”

Antibiofilm potential of a tropical marineBacillus licheniformisisolate: role in disruption of aquaculture associated biofilms

“…. Vibrio species are widely distributed in fresh water, estuarine and marine environments (Otta, Karunasagar & Karunasagar , ; Austin & Zhang ) and the salinity levels of these aquaculture systems vary during culture practices. Our studies on the effect of salinity on biofilm formation by V. harveyi , revealed that NaCl at 1% was optimum for biofilm formation.…”

IDENTIFICATION OF VIBRIO BACTERIA CAUSED VIBRIOSIS ON FRESHWATER PRAWN LARVAE (Macrobrachium rosenbergii (de Man))

“…Menurut Otta et al (2001), pada tingkat hatchery dapat terjadi peningkatan total populasi Vibrio hingga mencapai 60,3%. Hal tersebut menurut Sureshvarr et al (2011) dapat disebabkan karena Vibrio merupakan salah satu flora normal yang berasosiasi pada sistem hatchery.…”