Bacteria flora associated with different body parts of hatchery reared juvenilePenaeus monodon, tanks water and sediment

Abstract

Bacteria flora of intestine and hepatopancreas, body surface and muscles of juvenile Penaeus monodon along with its rearing water and sediment was analyzed. Juvenile shrimp were reared in four tanks in the Hatchery complex, Department of Aquaculture, Faculty of Agriculture, University Putra Malaysia. Water quality parameters were measured every day. Samples were collected aseptically and homogenized before being inoculated in Tryptone Soy agar, Thiosulphate Citrate Bile Salt agar, MacConkey agar and Pseudomonas-isolating agar. There was no significant difference between water quality parameters and shrimp body weight of replicate tanks. Total plate count for water and total Vibrio count for rearing water and digestive system were within previous reported ranges. Eight different genera were isolated in which 7 genera were identified. Gram negative bacteria were dominant (72%) Vibrio was the most dominant genera followed by Shewanella and Burkholderia. Clavibacter followed by Staphylococcus were the most dominant gram positive bacteria. No coliform bacteria was detected in the shrimp body parts and rearing environment. Incidence of Shewanella in the digestive system was significantly higher than sediment, rearing water and muscles. This may be implied its ability to colonize in the digestive tract of juvenile P. monodon.

Bacterial Community Associated with the Intestinal Tract of P. monodon in Commercial Farms

“…Other genus found in significant proportion in the intestinal bacterial community included Shewanella, Propionigenium, Fusibacter, Clostridium, and Blastopirellula. The cultureindependent methods in this study reveal similar dominant bacteria population to the previous culture-based studies done in black tiger shrimp P. monodon [56] and in other shrimp species [28,40]. Shakibazadeh et al [56] studied the bacterial community associated with different parts (intestines, hepatopancreas, body surface, and muscle) of juvenile P. monodon, rearing water, and tank sediments using culture-based isolation with several different agar media and found some similar species to the present experiment such as Vibrio, Shewanella, Burkholderia, Staphylococcus, and Corynebacterium.…”

“…The cultureindependent methods in this study reveal similar dominant bacteria population to the previous culture-based studies done in black tiger shrimp P. monodon [56] and in other shrimp species [28,40]. Shakibazadeh et al [56] studied the bacterial community associated with different parts (intestines, hepatopancreas, body surface, and muscle) of juvenile P. monodon, rearing water, and tank sediments using culture-based isolation with several different agar media and found some similar species to the present experiment such as Vibrio, Shewanella, Burkholderia, Staphylococcus, and Corynebacterium. However, many non-dominant phyla of bacteria observed in the present study such as Cyanobacteria, Fusobacteria, Planctomycetes, and Tenericutes were not previously reported in other studies that used culture-dependent methods to examine shrimp intestinal bacteria.…”

“…Most studies on the intestinal bacteria of P. monodon have employed culture-dependent method which does not take into account unculturable bacteria. Using plate count method, the intestinal bacterial community of juvenile P. monodon was dominantly composed of Shewanella followed by Vibrio and Staphylococcus, and other species isolated from the intestinal tract of P. monodon included Burkholderia, Clavibacter, and Brevibacterium [56]. In other penaeid species, the intestinal bacteria of wild and cultured banana prawn Penaeus merguiensis examined using plate count method were dominated by Vibrio, and other species obtained were Aeromonas, Plesiomonas, Photobacterium, Pseudoalteromonas, and Pseudomonas [40].…”

Rapid Change of Microbiota Diversity in the Gut but Not the Hepatopancreas During Gonadal Development of the New Shrimp Model Neocaridina denticulata

“…First, majority of the aforementioned studies focused on examining the bacterial community in the gut, and there was only one study that also studied the hepatopancreas or midgut gland (Shakibazadeh et al 2009), which is an important organ responsible for digestion, absorption, and storage of nutrients in crustaceans that also inhabits pathogens (Ceccaldi 1989;Leaño et al 1998;Jiang et al 2014). Another issue is that none of these studies has compared the microbiota in different parts of the gut during gonad development of the host.…”

“…clone library analysis and denaturing gradient gel electrophoresis (DGGE), intestinal bacterial communities have been examined in various crustaceans, including the black tiger shrimp Penaeus monodon (Shakibazadeh et al 2009;Chaiyapechara et al 2012), red-tailed shrimp Penaeus penicillatus , Chinese shrimp Penaeus chinensis (Liu et al 2011), Atlantic blue crab Callinectes sapidus (Givens et al 2013), the cladoceran Daphnia magna (Freese and Schink 2011), and the copepod Eudiaptomus gracilis (Homonnay et al 2012). Advancement of nextgeneration sequencing (NGS) platforms has also facilitated more in-depth investigations into the gut microbiota of P. monodon (Rungrassamee et al 2013), the white shrimp Penaeus vannamei Huang et al 2015), and Norvegian lobster Nephrops norvegicus (Meziti and Kormas 2013).…”