Effects of exposure to bisphenol A during pregnancy and lactation on the testicular morphology and caspase-3 protein expression of ICR pups

Liu, Xiaoli; Chen, Xiaoyu; Wang, Zhi‐Cheng; Shen, Tong; Zhao, Huna

doi:10.3892/br.2013.79

Cited by 27 publications

(29 citation statements)

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“…However, the BPA-treated group showed a statistically significant increase in the number of caspase-3-positive cells in comparison with that of the control. Similarly, increased apoptosis with elevated expression of caspase-3 was detected in the testes of mice pups exposed to BPA during pregnancy and lactation (Liu et al 2013). Moreover, similar findings were observed in the liver of rats treated with BPA (Xia et al 2014).…”

Section: Discussionsupporting

confidence: 71%

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Effect of bisphenol A on morphology, apoptosis and proliferation in the resting mammary gland of the adult albino rat

Ibrahim

Elbakry

Bayomy

2016

Int J Experimental Path

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SUMMARYBisphenol A (BPA) is a synthetic oestrogen that is extensively used in a wide range of daily used plastic products. This makes it one of the environmental chemicals that may have impact on human health. Due to its oestrogenic effect, BPA might affect the mammary gland. This study aimed to investigate the influence of BPA on the histological structure of the mammary gland of the adult female albino rat and its effect on epithelial cell proliferation and apoptosis status, in addition to its possible modulating effect on estrogen receptor expression. Thirty female adult albino rats were divided into control and experimental groups. The rats in the experimental group were gavaged with 5 mg/kg BPA daily for 8 weeks. The mammary glands were dissected and processed for histological and immunohistochemical stains for Ki-67, activated caspase-3 and estrogen receptor alpha (ER-a). BPA induced an increase in the number and size of the acini and ducts in the mammary gland of treated rats with hyperplasia of their lining epithelial cells. The collagen fibre content was significantly increased in the connective tissue stroma separating the ducts. Immunohistochemical results showed a significant increase in Ki-67 and caspase-3, but a nonsignificant increase in ER-a expression. Bisphenol A induced structural changes and affected the proliferation rate of mammary glands, so it might be one of the predisposing factors for breast cancer.

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Section: Discussionsupporting

confidence: 71%

“…Similarly, increased apoptosis with elevated expression of caspase‐3 was detected in the testes of mice pups exposed to BPA during pregnancy and lactation (Liu et al . ). Moreover, similar findings were observed in the liver of rats treated with BPA (Xia et al .…”

Section: Discussionmentioning

confidence: 97%

Effect of bisphenol A on morphology, apoptosis and proliferation in the resting mammary gland of the adult albino rat

Ibrahim

Elbakry

Bayomy

2016

Int J Experimental Path

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“…Peritubular myoid cells maintain the morphology of the seminiferous tubules participating in the formation of the basement membrane. It was showed, that exposure in prenatal life to BPA resulted in apoptosis of pertitubular myoid cells in testis of 21 days old mice [70]. The same effect was observed in adult rats treated with cimetidine which has weak antiandrogenic properties [71].…”

Section: Discussionsupporting

confidence: 50%

The immunoexpression of androgen receptor, estrogen receptors alpha and beta, vanilloid type 1 receptor and cytochrome p450 aromatase in rats testis chronically treated with letrozole, an aromatase inhibitor

Pilutin

Misiakiewicz-Has²,

Kolasa³

et al. 2014

Folia Histochem Cytobiol.

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Abstract:The function of testis is under hormonal control and any disturbance of hormonal homeostasis can lead to morphological and physiological changes. Therefore the aim of the study was to investigate the expression of androgen and estrogen receptors (AR, ERs), vanilloid receptor (TRPV1), cytochrome P450 aromatase (P450arom), as well as apoptosis of cells in testis of adult rats chronically treated with letrozole (LT), a non-steroidal aromatase inhibitor, for 6 months. The testicular tissues were fixed in Bouin's fixative and embedded in paraffin. Immunohistochemistry with monoclonal antibodies (abs) against AR, ERa, P450arom, and polyclonal abs against ERb, TRPV1, caspase-3 was applied. Long-lasting estradiol deficiency, as an effect of LT treatment, produced changes in the morphology of testis and altered the expression of the studied receptors in cells of the seminiferous tubules and rate of cell apoptosis. The immunostaining for AR was found in the nuclei of Sertoli cells and the cytoplasm of spermatogonia and spermatocytes in III-IV stages of the seminiferous epithelium cycle. The intensity of staining for P450arom was lower in the testis of LT-treated rats as compared to control animals. The immunofluorescence of ERa and ERb was observed exclusively in the nuclei of Leydig cells of LT-treated rats. There were no changes in localization of TRPV1, however, the intensity of reaction was stronger in germ cells of the seminiferous epithelium after LT treatment. The apoptosis in both groups of animals was observed within the population of spermatocytes and spermatids in II and III stages of the seminiferous epithelium cycle. In testis of LT-treated rats the immunoexpression of caspase-3 was additionally found in the germ cells in I and IV stages, and Sertoli, myoid and Leydig cells. In conclusion, our results underline the important role of letrozole treatment in the proper function of male reproductive system, and additionally demonstrate that hormonal imbalance can produce the morphological abnormalities in testis.

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“…A number of studies point out that the adult exposure BPA can reduce sperm production and efficiency of spermatogenesis [33,34] . Liu et al found that exposure of pregnant mice to BPA during pregnancy and lactation has some toxic effects on the testes of male offspring and these may originate from increased apoptosis [35] .…”

Section: Discussionmentioning

confidence: 99%

The possible protective effect of Omega 3 fatty acids against Bisphenol A induced disruption of pituitary-testicular axis in albino rat. Biohemical, Histological and Immunohistochemical study

Mahmoud

Nor-Eldin

Elsayed

2019

Egyptian Journal of Histology

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Background: Infertility is a major health problem affecting human life. Multiple factors contribute to male infertility. The most important one is exposure to environmental contaminants (e.g. Bisphenol A (BPA)). Aim of the work: To study the possible protective effect of Omega 3 against BPA induced changes in the pituitary testicular axis in rats. Material and method: 30 adult male albino rats were used in the study. They were divided equally into 3 groups 10 animals each. Group I served as control. Group II received BPA in a dose 1.2 mg/kg orally 6 days a week. Group III received Omega 3 in a dose 0.4 g/kg subcutaneously in addition to BPA of the same previous dose and duration. At the time of sacrifice, all rats were anesthetized with ether.Blood samples were collected for estimation of testosterone, LH, FSH and prolactin. The testes and pituitary were dissected out and processed for histological and immunohistochemical study for Caspase 3, PCNA and prolactin. The number of Caspase 3 and PCNA positive cells were counted and statistically analyzed. Results: Marked decrease in serum LH, testosterone with marked increase in serum prolactin was observed in BPA treated group. Spermatogenic cells were disorganized and degenerated. Spermatids had fragmented pyknotic nuclei. A significant increase in the rate of apoptosis and a significant decrease in the rate of proliferation of germ cells were detected on BPA exposure compared to the control. Examination of pars distalis revealed degenerative changes in acidophils and basophils in group II animals with decreased intensity of reactive mammotrophs. These changes were ameliorated in group III by administration of Omega 3 fatty acids. Conclusion: Omega 3 may protect against BPA hazardous effects through pituitary testicular axis pathway.

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Effects of exposure to bisphenol A during pregnancy and lactation on the testicular morphology and caspase-3 protein expression of ICR pups

Cited by 27 publications

References 28 publications

Effect of bisphenol A on morphology, apoptosis and proliferation in the resting mammary gland of the adult albino rat

Effect of bisphenol A on morphology, apoptosis and proliferation in the resting mammary gland of the adult albino rat

The immunoexpression of androgen receptor, estrogen receptors alpha and beta, vanilloid type 1 receptor and cytochrome p450 aromatase in rats testis chronically treated with letrozole, an aromatase inhibitor

The possible protective effect of Omega 3 fatty acids against Bisphenol A induced disruption of pituitary-testicular axis in albino rat. Biohemical, Histological and Immunohistochemical study

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