Effects of Exogenous Putrescine on Delaying Senescence of Cut Foliage of Nephrolepis cordifolia

Abstract

Senescence is the main limitation for cut foliage display in vase. Naturally occurring polyamines such as putrescine (Put) have been considered effective anti-senescence agents. However, effect of Put on cut foliage in vase in a realistic indoor environment has not yet been revealed. In the present study, effects of Put spraying on the postharvest performance of cut foliage of Nephrolepis cordifolia L. were investigated. Cut fronds sprayed with deionized water (Put0) showed visible injuries after 10 days in vase. Meanwhile, chlorophyll (Chl), soluble protein (Sp), and proline (Pro) content were decreased by 60.15, 57.93, and 73.09% respectively, photochemical activity reflected by Chl fluorescence parameters was inhibited, whereas electrolyte leakage (EL), contents of soluble sugar (Ss), malondialdehyde (MDA), and hydrogen peroxide (H 2 O 2) were increased (+194.29, +44.83, +34.06, and +178.01%, respectively). Put spraying extended the vase life of the cut foliage and the 2.0 mM Put had a longer vase life (21 days) than 0.2 mM (15 days). Leaf spraying of 2.0 mM Put for 10 days significantly ameliorated the losses of Chl, Sp, and Pro content (−10.72, −26.29, and −42.64%, respectively), followed by 0.2 mM Put (−27.36, −36.24, and −60.55%, respectively). Put spraying also improved photochemical capability and prevented membrane impairment as well as visible injury in comparison with Put0. In addition, 2.0 mM Put had a better mitigating ability than that of 0.2 mM. Leaf spraying of 2.0 mM Put greatly reduced the decline of the effective quantum yield of photochemical energy conversion in PSII (FPSII), the maximal quantum yield of PSII photochemistry measured in the dark-adapted state (Fv/Fm) and electron transport rate (ETR) (−7.89, −12.91, and −10.06%, respectively), and also inhibited the increases of EL, MDA, Ss, and H 2 O 2 (+31.87, +6.43, +16.22, and +49.40%, respectively). Overall, Put played important roles in deterring the degradation of Chl, Ss, and Pro, detoxifying the H 2 O 2 , weakening the sugar signaling, mitigating the decline of photochemical activity, and eventually postponing the leaf senescence. The present study gives new insights into effects of Put on leaf senescence and provides a strategy for preserving post-harvest cut foliage.

Exogenous Putrescine Alleviates Drought Stress by Altering Reactive Oxygen Species Scavenging and Biosynthesis of Polyamines in the Seedlings of Cabernet Sauvignon

“…Exogenous Put significantly increased soluble sugar accumulation under drought stress, which is closely related to a signaling adjustment to environmental response. It is likely because Put is polycationic, meaning it can bind and stabilize osmotic micromolecules ( Qu et al, 2020 ). Sucrose metabolism is often used as an indicator of environmental stress and plant adaptability.…”

Navigating the future: exploring technological advancements and emerging trends in the sustainable ornamental industry

“…They are involved in various physiological processes, such as cell division, differentiation, and stress response. Recent studies have shown that treating cut flowers with polyamines, such as putrescine and spermidine, can improve their vase life by reducing senescence and preserving flower quality (Qu et al, 2020;Mazrou et al, 2022). Chitosan is a biodegradable polymer derived from chitin, which is found in the shells of crustaceans.…”

Review of the Mechanisms by Which Transcription Factors and Exogenous Substances Regulate ROS Metabolism under Abiotic Stress

“…Polyamines, putrescine (Put), spermidine (Spd) and spermine (Spm), are widely present in plants and are closely related to plant growth and development. Qu et al found that exogenous Put significantly ameliorated the losses of Chl and improved photochemical capability and prevented membrane impairment of Nephrolepis cordifolia [ 208 ]. Tavallali et al reported exogenous Spd significantly increased levels of phenolic and flavonoid compounds in pot marigold [ 209 ].…”