Effects of exogenous application of polyamines on wheat anther cultures

Redha, Amina; Suleman, Patrice

doi:10.1007/s11240-010-9873-7

Cited by 27 publications

(27 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genotype dependency and albinism limit the wide application of anther cultures in practical wheat breeding (Rybczyński et al, 1991;Marciniak et al, 2003;Broughton, 2011;Redha and Suleman, 2011;Lantos et al, 2013). In this study, the frequency of formation of embryo-like structure was shown to be influenced by the genetic background.…”

Section: Discussionmentioning

confidence: 58%

Obtaining doubled haploid lines of the Lr19 gene using anther cultures of winter wheat genotypes

Weigt

Kiel

Tomkowiak

et al. 2016

bta

View full text Add to dashboard Cite

Leaf rust pathogens are able to overcome plants' resistance by the appearance of new races. Therefore, an introduction of leaf rust resistance genes into highyielding wheat genotypes is essential. One of such genes is Lr19 (leaf rust resistance gene) that could be a source of resistance in polish breeding programs. The aim of this study was to obtain doubled haploid lines of the Lr19 gene in anther cultures of winter wheat genotypes. In the experiment, F 3 generation plants from two cross-combinations were used: genotype T39 × Ozon and genotype T36 × Hondia (T36 and T39 lines carry Lr19 gene). Three combinations of growth regulators such as 2,4-D; 2,4-D and dicamba; and 2,4-D and kinetin were applied. A total of 13,550 anthers were tested. The embryo-like structures were formed at an average frequency of 9.95% and were mainly influenced by the genotype, not by the composition of hormones in induction media. Regeneration frequency of green plants was recorded to be 1.45%. The highest frequency of green plant regeneration was observed on induction media supplemented with 2,4-D and dicamba reaching 1.79%. Spontaneous doubled haploids tested by laser flow cytometry were detected in 33.95% of plants. The frequency of the formation of embryo-like structure was mainly influenced by the genotype, not by the composition of hormones in induction media.

show abstract

Section: Discussionmentioning

confidence: 58%

Obtaining doubled haploid lines of the Lr19 gene using anther cultures of winter wheat genotypes

Weigt

Kiel

Tomkowiak

et al. 2016

bta

View full text Add to dashboard Cite

show abstract

“…Polyamines retard aging, and thus may contribute to increasing the efficiency of androgenetic embryogenesis. In the latest publication among those discussed here, Redha and Suleman (2011) obtained an increase in the number of what they called embryo-like structures (ELSs) under the influence of spermidine, putrescine, and spermine, which were used to treat anthers for 1, 3, and 6 h. Although the exogenous application of polyamines to anthers improved the production of ELS and green plants, the effects of putrescine, spermidine, and spermine were dependent on the genotype and the duration of the pretreatment of anthers with polyamines.…”

Section: Discussionmentioning

confidence: 97%

Effect of polyamines on in vitro anther cultures of carrot (Daucus carota L.).

Górecka¹,

Kiszczak²,

Krzyżanowska³

et al. 2014

Turk J Biol

View full text Add to dashboard Cite

This study examines the influence of the polyamines putrescine and spermidine on the efficiency of androgenesis in anther cultures of 2 carrot cultivars, Kazan F 1 and Narbonne F 1 , and the effect of putrescine on the process of plant regeneration from androgenetic embryos. In the Kazan F 1 variety, an increase in the number of obtained embryos was achieved using each of the 2 polyamines separately and in combination. In contrast, no beneficial effects of polyamines on the efficiency of androgenesis were observed in the Narbonne F 1 variety. Putrescine added to the regeneration medium increased the number of obtained plants. For embryos obtained on induction medium without putrescine, the best concentration was 160 mg putrescine in 1 L of regeneration medium, and for those obtained on the induction medium with putrescine the best concentration was 0.5 mg putrescine per 1 L. All of the resulting plants, both in the experimental and control combinations, had a doubled set of chromosomes. The vast majority of them were homozygous for both isoenzymes, glucose-6-phosphate isomerase and aspartate aminotransferase, and the distribution of homo-and heterozygous received combinations with the polyamines and in the control was very similar.

show abstract

“…The other two lines ICARDA17 and ICARDA39 were from the International Center for Agricultural Research in the Dry Areas (ICARDA), Aleppo, Syria. The anther culture response for a number of ICARDA genotypes including ICARDA17 and 39 was examined in an earlier study [10]. Seeds were germinated at 25˚C and after 10 days seedlings were planted in peat moss in 10 cm diameter pots, then placed in growth chambers at a temperature cycle of 23/18˚C day/night, 16 h photoperiod and 290 -310 µmol·m Spikes were collected when microspores were at midto late-uninucleate stage [26].…”

Section: Plant Materialsmentioning

confidence: 99%

“…Thus, in wheat, microspore culture manipulation of conventional protocols physically and/or chemically is one approach to maximize the production of green plants [9]. Exogenous application of polyamines (PAs) to wheat anthers improved the green plant regeneration but overall effect was dependent on duration of the pretreatment and on the genotype [10].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Assessment of Polyamines and Trehalose in Wheat Microspores Culture for Embryogenesis and Green Regenerated Plants

Redha¹,

Suleman²

2013

AJPS

Self Cite

View full text Add to dashboard Cite

Most aspects of microspore culture protocol have the capacity to cause stress to microspores, hence, less stressful treatments might be required to avoid deleterious effects. In stressed plants, polyamines and trehalose can act as compatible solutes or osmoprotectants by stabilizing proteins and biological membranes. To improve green plant regeneration in wheat microspore culture, this study assessed the effects of polyamines (putrecine, spermidine, spermine) and trehalose on androgenic response namely embryogenesis, green plant regeneration and ploidy of green plants regenerated in three spring wheat genotypes. Microspores of the genotypes produced significant numbers of embryos and green plants among polyamine treatments but trehalose had no effect (P ≤ 0.05). Polyamine treatments for 30 min generally produced more green plants per 100 microspores than the 60 min treatments in all three genotypes. At least three out of twelve polyamine treatments in each genotype improved the production of double haploid plants and seed setting in regenerants. Wheat genotype, concentration and duration of polyamine treatment had significant impact on embryogenesis and regeneration of green plants in this study. The study also showed that polyamines could be used to accelerate cultivar development in wheat breeding.

show abstract

Effects of exogenous application of polyamines on wheat anther cultures

Cited by 27 publications

References 42 publications

Obtaining doubled haploid lines of the Lr19 gene using anther cultures of winter wheat genotypes

Obtaining doubled haploid lines of the Lr19 gene using anther cultures of winter wheat genotypes

Effect of polyamines on in vitro anther cultures of carrot (Daucus carota L.).

Assessment of Polyamines and Trehalose in Wheat Microspores Culture for Embryogenesis and Green Regenerated Plants

Contact Info

Product

Resources

About