Assessment of Polyamines and Trehalose in Wheat Microspores Culture for Embryogenesis and Green Regenerated Plants

Abstract

Most aspects of microspore culture protocol have the capacity to cause stress to microspores, hence, less stressful treatments might be required to avoid deleterious effects. In stressed plants, polyamines and trehalose can act as compatible solutes or osmoprotectants by stabilizing proteins and biological membranes. To improve green plant regeneration in wheat microspore culture, this study assessed the effects of polyamines (putrecine, spermidine, spermine) and trehalose on androgenic response namely embryogenesis, green plant regeneration and ploidy of green plants regenerated in three spring wheat genotypes. Microspores of the genotypes produced significant numbers of embryos and green plants among polyamine treatments but trehalose had no effect (P ≤ 0.05). Polyamine treatments for 30 min generally produced more green plants per 100 microspores than the 60 min treatments in all three genotypes. At least three out of twelve polyamine treatments in each genotype improved the production of double haploid plants and seed setting in regenerants. Wheat genotype, concentration and duration of polyamine treatment had significant impact on embryogenesis and regeneration of green plants in this study. The study also showed that polyamines could be used to accelerate cultivar development in wheat breeding.

Proline and chitosan enhanced efficiency of microspore embryogenesis induction and plantlet regeneration in Brassica napus L.

“…The accumulation of compatible solutes is often regarded as a basic strategy for the protection and survival of cells under stressful conditions, including the stresses used for embryogenesis induction (Patnaik et al 2005;Chen et al 2007;Redha and Suleman 2013). According to our results, proline was not advantageous to microspore embryogenesis when maintained for 1 day in the induction medium, but a 30-55 % increase in the embryo formation was observed in response to 100 mg l -1 proline in the cultures incubated for 2 and 5 days.…”

Androgenesis in indica rice: A comparative competency in development of doubled haploids

“…Vitamins and amino acids are the preferential organic nitrogen source in the culture media. Under stress condition plants tends to accumulate several metabolites and amino acids specially proline, which is a common strategy for the protection and survival of cells under such conditions including callus induction, green shoot regeneration and embryogenesis [ 53 – 55 ]. This study showed increment of green shoot regeneration in proline fortified media for all tested genotypes except IR20 X Mahulata.…”