Effect of polyamines on in vitro anther cultures of carrot (Daucus carota L.).

Górecka, Krystyna; Kiszczak, Waldemar; Krzyżanowska, D.; Kowalska, Urszula; Kapuścińska, Agata

doi:10.3906/biy-1403-29

Cited by 12 publications

(4 citation statements)

References 29 publications

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“…Dewi et al (2001) reported that putrescine was more efficient than spermidine and spermine in callus induction and plant regeneration in rice anther culture. Similar observations were reported in several plant species such as P. ginseng (Kevers et al, 2000), G. hirsutum (Sakhanokho et al, 2005), M. charantia (Paul et al, 2009), and Daucus carota (Gorecka et al, 2014).…”

Section: Discussionsupporting

confidence: 87%

Effect of polyamines on somatic embryogenesis via mature embryo in wheat

Aydın¹,

Türkoğlu²,

Haliloğlu³

et al. 2016

Turk J Biol

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Plant tissue culture via somatic embryogenesis plays a key role in wheat genetic transformation studies. Therefore, the production of embryogenic calli with a high regeneration capacity is a prerequisite for efficient plant regeneration. Although immature embryos are the best type of explants for plant regeneration via somatic embryogenesis, the use of mature embryos has remarkable advantages compared to immature embryos as explants. However, the regeneration capacity of mature embryos is still lower than that of immature embryos. Plant regeneration from somatic embryos can only occur when they are mature enough. Moreover, the maturation of somatic embryos and their conversion into plantlets is closely associated with the used plant growth regulators. Polyamines are major components in the formation of somatic embryogenesis and plant regeneration. In this study, the effect of three types of polyamines on somatic embryogenesis and plant regeneration in wheat was evaluated. Although the effect of polyamines on embryogenic callus formation was not significant, their effect on responded embryogenic callus rate and plant regeneration efficiency was very significant. The highest responded embryogenic callus rate and highest regeneration efficiency were obtained on MS medium containing putrescine. Unlike other polyamines, an increase in putrescine concentration promoted a higher number of regenerated plants. The highest values for responded embryogenic callus and regeneration efficiency were determined in 1 mM concentration of putrescine. Plantlets derived from somatic embryos were successfully established in soil, producing fertile seeds.

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Section: Discussionsupporting

confidence: 87%

Effect of polyamines on somatic embryogenesis via mature embryo in wheat

Aydın¹,

Türkoğlu²,

Haliloğlu³

et al. 2016

Turk J Biol

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“…Hoverer, exogenous polyamines can regulate growth and development, including embryogenesis and organogenesis in vitro [40]. Górecka and co-workers [30] examined the effect of the exogenous polyamines, such as putrescine and spermidine supplemented into B5 media, on the efficiency of androgenesis in anther cultures of two carrot cultivars. The authors obtained over 250% more embryos on the medium with the addition of putrescine, and over 40% more on the medium supplemented with spermidine, in comparison to the control.…”

Section: Anther Culturementioning

confidence: 99%

History and Current Status of Haploidization in Carrot (Daucus carota L.)

Kiełkowska

Kiszczak

2023

Agronomy

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The induction of haploid cell development into normal plants enables the production of doubled haploid lines, which are homozygous and can be used in breeding programs as an alternative to conventionally derived inbred lines. In this paper, we present the historical background and current status of the attempts of haploid induction in carrot (Daucus carota L.). Economically, carrot is one of the most important vegetables. It is an outcrossing diploid (2n = 2x = 18) species. Nowadays, the seeds of hybrid cultivars constitute the majority of the carrot seeds sold in the world. Hybrid cultivars of carrot are produced using inbred populations. Inbreeding in this species is difficult due to an inbreeding depression and is also time-consuming, as it is a biennial crop. Therefore, the implementation of the haploidization technology into the breeding programs of carrot is of high interest. Androgenesis, gynogenesis and induced parthenogenesis are the methods that have been used for haploid induction, and their potential in haploidization of carrot is discussed. The centromere-specific histone 3 variant (CENH3) and its manipulation in carrot is also acknowledged.

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“…Moreover, the promoter role of polyamines in the conversion of somatic embryos or shoot regeneration has been evidenced in several plant species (Górecka et al, 2014;Aydin et al, 2016). Hegazy and Aboshama (2010)…”

Section: Introductionmentioning

confidence: 99%

“…induced direct somatic embryogenesis from bud tissues of date palm through putrescine. Similarly,Kumar et al (2004) applied putrescine and spermidine as an addition to the induction medium of cucumbers, whereasGórecka et al (2014) reported that the addition of putrescine, spermidine, and a mixture of the both to the induction medium had a beneficial effect on the number of embryos in Daucus carota. The results of present study are in line with the above-mentioned studies where the addition of polyamines enhanced somatic embryos formation.ConclusionIt can be concluded from the present findings that theaddition of putrescine in combination with higher concentration of glutamine (EA) has positive embryogenetic potential in date palm cv.…”

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confidence: 99%

Somatic embryogenesis enhancement of date palm cultivar Sewi using different types of polyamines and glutamine amino acid concentration under in-vitro solid and liquid media conditions

2018

Int. J. Biosci.

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Date palm is traditionally propagated by ground off-shoots, which are in a limited number and varied with the cultivar. The production of date palm through tissue culture is well established due to the advantages of rapid clonal production, genetically uniformity and pathogen-free plants. The source explants are grown in a solid or liquid media. The media formulation and amendments play a vital role to establish a successful tissue culture protocol for explant regeneration and multiplication. Therefore, the rational of present study was to enhance somatic embryos induction of date palm cv. Sewi from embryonic callus during maturation stage by adding polyamines and glutamine amino acid concentrations in solid or liquid media. Two types of polyamines, putrescine and spermidine (each at 100 mg.L -1 concentration) along with basic concentration of glutamine amino acid (B) (200 mg.L -1 ) or with the extra addition of glutamine concentration (EA) (100 mg.L -1 ) were included in the study to develop micropropagation cycle of date palm plantlets. Explants cultured on solid media showed excellent embryonic callus whereas liquid media was best for somatic embryos induction. The quantitative observation indicated that maximum number of somaticembryos (32.33) were counted in liquid mediacontaining glutamine (EA) with putrescine. On the other hand, highest embryonic callus fresh weight was produced by the explants contained spermidinealone (5.49 g) and glutamine (EA) + spermidine (5.30 g) in solid media. It was concluded that putrescine with glutamine (EA) orits combination with spermidinealong with glutamine (B) in the solid or liquid in-vitromedia were the best for embryonic callus maturation to enhance the somatic embryogenesis of date palm cv. Sewi.

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Effect of polyamines on in vitro anther cultures of carrot (Daucus carota L.).

Cited by 12 publications

References 29 publications

Effect of polyamines on somatic embryogenesis via mature embryo in wheat

Effect of polyamines on somatic embryogenesis via mature embryo in wheat

History and Current Status of Haploidization in Carrot (Daucus carota L.)

Somatic embryogenesis enhancement of date palm cultivar Sewi using different types of polyamines and glutamine amino acid concentration under in-vitro solid and liquid media conditions

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