Effects of estrogenic xenobiotics on human and mouse spermatozoa

Fraser, Lynn R.; Beyret, Ergin; Milligan, S. R.; Adeoya-Osiguwa, Susan

doi:10.1093/humrep/dei486

Cited by 89 publications

(47 citation statements)

References 53 publications

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“…Although 17a-ethynylestradiol only seems to act at higher concentrations (R2 ng/ml), the combination of all substances with potential or confirmed estrogenic activity can further misbalance the physiology of epididymal spermatozoa. Furthermore some artificial compounds with estrogenic activity have a significant effect on sperm physiology at lower doses compared with the strongest natural estrogen such as E 2 (Fraser et al 2006, Sebkova et al 2012.…”

Section: Discussionmentioning

confidence: 99%

In vivo exposure to 17β-estradiol triggers premature sperm capacitation in cauda epididymis

Děd¹,

Šebková²,

Černá³

et al. 2013

Reproduction

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Estrogens play a crucial role in spermatogenesis and estrogen receptor a knock-out male mice are infertile. It has been demonstrated that estrogens significantly increase the speed of capacitation in vitro; however this may lead to the reduction of reproductive potential due to the decreased ability of these sperm to undergo the acrosome reaction. To date the in vivo effect of estrogens on the ability of sperm to capacitate has not been investigated. Therefore, in this study, we exposed mice (nZ24) to 17b-estradiol (E 2 ) at the concentration of 20 ng/ml either during puberty from the fourth to seventh week of age (nZ8), or continuously from birth for a period of 12 weeks (nZ8) at which age the animals from both groups were killed. The capacitation status of epididymal and testicular sperm was analysed by tyrosine phosphorylation (TyrP) antibody (immunofluorescence and western blot) and chlortetracycline (CTC) assay. According to our results, in vivo exposure to increased E 2 concentrations caused premature sperm capacitation in the epididymis. The effect of E 2 , however, seems reversible because after the termination of the exposure premature epididymal sperm capacitation is decreased in animals treated during puberty. Furthermore the changes in epididymal sperm capacitation status detected by TyrP and CTC positively correlate with plasma levels of E 2 and the expression of the estrogen-dependent trefoil factor 1 (Tff1) gene in testicular tissue. Therefore, our data implicate that in vivo exposure to E 2 under specific conditions leads to the premature capacitation of mouse sperm in epididymis with a potential negative impact on the sperm reproductive fitness in the female reproductive tract.

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Section: Discussionmentioning

confidence: 99%

In vivo exposure to 17β-estradiol triggers premature sperm capacitation in cauda epididymis

Děd¹,

Šebková²,

Černá³

et al. 2013

Reproduction

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“…In our experimental conditions, we detected a slight reduction in the percentage of viable sperm with high lipid membrane disorder. The role of genistein in capacitation is not well defined; Fraser et al [49] reported that genistein at low concentrations (1, 10 and 100 nmol L -1 ) accelerated the capacitation and acrosome loss in human spermatozoa measured by CTC staining. On the other hand, several studies have shown the capacity of genistein taken at a higher concentration to inhibit the progesterone-induced acrosome reaction [50][51][52].…”

Section: Resultsmentioning

confidence: 99%

Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

Martínez-Soto¹,

DiosHourcade²,

Gutiérrez‐Adán³

et al. 2010

Asian J Androl

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In this study, we evaluated the effects of genistein supplementation of the thawing extender on frozen-thawed human semen parameters. We analyzed the effect of supplementation on sperm motility, capacitation (membrane lipid disorder), reactive oxygen species (ROS) generation, chromatin condensation and DNA damage. Using this preliminary information, it maybe possible to improve the cryopreservation process and reduce the cellular damage. We have confirmed that the isoflavone genistein (10 mmol L -1 ) has antioxidant properties on the frozen-thawed spermatozoa. This results in a decreased ROS production that shows a slight improvement in the sperm motility, and decreases the membrane lipid disorder and DNA damage caused by cryopreservation. These results suggest an effect of genistein on sperm functionality that could be of interest for assisted reproduction treatments using frozenthawed human spermatozoa, but further studies will be necessary to confirm our findings and to evaluate the possible clinical applications.

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“…genistein, daidzein, glycitein), for 2 months (Mitchell et al, 2001). To add more confusion, an in vitro study showed that low concentrations of genistein (1, 10 and 100 nM) caused an accelerated capacitation and acrosome loss in human spermatozoa (Fraser et al, 2006).…”

Section: Soy Phytoestrogens and Male Reproductive Functionmentioning

confidence: 99%

“…Phytoestrogens appear to act in a similar manner. In vitro experiments have shown that low concentrations of genistein, nonylphenol and 8-pre-nylnaringenin were more effective when used in combinations, rather than alone, in causing adverse effects on the key processes of capacitation and acrosome reaction both in human and mouse spermatozoa (Fraser et al, 2006). Recently, it has been reported in rat studies that alterations in male reproductive functions were more pronounced when co-exposed to a low dose of genistein (1 mg/kg/day) and the fungicide vinclozolin (1 mg/kg/day) from conception to adulthood (Eustache et al, 2009).…”

Section: Soy Phytoestrogens and Male Reproductive Functionmentioning

confidence: 99%

Soy, phytoestrogens and their impact on reproductive health

Cederroth

Zimmermann

Nef

2012

Molecular and Cellular Endocrinology

189

117

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a b s t r a c tThere is growing interest in the potential health threats posed by endocrine-disrupting chemicals (EDCs) to the reproductive system. Soybean is the most important dietary source of isoflavones, an important class of phytoestrogen. While consumption of soy food or phytoestrogen supplements has been frequently associated with beneficial health effects, the potentially adverse effects on development, fertility, and the reproductive and endocrine systems are likely underappreciated. Here we review the available epidemiological, clinical and animal data on the effects of soy and phytoestrogens on the development and function of the male and female reproductive system, and weigh the evidence as to their detrimental impact.

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Effects of estrogenic xenobiotics on human and mouse spermatozoa

Cited by 89 publications

References 53 publications

In vivo exposure to 17β-estradiol triggers premature sperm capacitation in cauda epididymis

In vivo exposure to 17β-estradiol triggers premature sperm capacitation in cauda epididymis

Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

Soy, phytoestrogens and their impact on reproductive health

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