Effects of Enzymatic Degradation after Loading in Temporomandibular Joint

Asakawa-Tanne, Yuki; Su, Shao-Ching; Kunimatsu, Ryo; Hirose, Naoto; Mitsuyoshi, Tomomi; Okamoto, Yuki; Tanaka, Eiji; Tanne, Kazuo; Tanimoto, Kotaro

doi:10.1177/0022034514560588

Cited by 45 publications

(32 citation statements)

References 31 publications

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“…We previously investigated the mechanism involved in OA and reported that the protein lubricant superficial zone protein (SZP) localizes on the temporomandibular joint surface [12]. We also demonstrated that excessive mechanical loading lowers SZP production [13]; increases the friction coefficient; increases MMP-1, 3, and 9 production; and destroys substrates such as type II collagens, aggrecan, and hyaluronic acid [14,15].…”

Section: Introductionmentioning

confidence: 99%

High-Frequency Near-Infrared Diode Laser Irradiation Attenuates IL-1β-Induced Expression of Inflammatory Cytokines and Matrix Metalloproteinases in Human Primary Chondrocytes

Sakata

Kunimatsu

Tsuka

et al. 2020

JCM

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High-frequency near-infrared diode laser provides a high-peak output, low-heat accumulation, and efficient biostimulation. Although these characteristics are considered suitable for osteoarthritis (OA) treatment, the effect of high-frequency near-infrared diode laser irradiation in in vitro or in vivo OA models has not yet been reported. Therefore, we aimed to assess the biological effects of high-frequency near-infrared diode laser irradiation on IL-1β-induced chondrocyte inflammation in an in vitro OA model. Normal Human Articular Chondrocyte-Knee (NHAC-Kn) cells were stimulated with human recombinant IL-1β and irradiated with a high-frequency near-infrared diode laser (910 nm, 4 or 8 J/cm2). The mRNA and protein expression of relevant inflammation- and cartilage destruction-related proteins was analyzed. Interleukin (IL) -1β treatment significantly increased the mRNA levels of IL-1β, IL-6, tumor necrosis factor (TNF) -α, matrix metalloproteinases (MMP) -1, MMP-3, and MMP-13. High-frequency near-infrared diode laser irradiation significantly reduced the IL-1β-induced expression of IL-1β, IL-6, TNF-α, MMP-1, and MMP-3. Similarly, high-frequency near-infrared diode laser irradiation decreased the IL-1β-induced increase in protein expression and secreted levels of MMP-1 and MMP-3. These results highlight the therapeutic potential of high-frequency near-infrared diode laser irradiation in OA.

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Section: Introductionmentioning

confidence: 99%

High-Frequency Near-Infrared Diode Laser Irradiation Attenuates IL-1β-Induced Expression of Inflammatory Cytokines and Matrix Metalloproteinases in Human Primary Chondrocytes

Sakata

Kunimatsu

Tsuka

et al. 2020

JCM

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“…Further evidence is provided from another study which demonstrated that under mechanical loading conditions, there is a release of pro-inflammatory and matrix degradation mediators which are associated with altered frictional properties and surface wear of condylar cartilage (5). …”

Section: Discussionmentioning

confidence: 99%

“…Indeed, compromised lubrication in the TMJ is associated with altered frictional properties and surface wear of the condylar cartilage, which is accompanied by the release of pro-inflammatory and matrix degradation mediators under mechanical loading conditions (5). Several molecules that are present in the SF contribute to joint lubrication, particularly boundary lubrication; one such molecule is lubricin (4,6).…”

Section: Introductionmentioning

confidence: 99%

Lubricin in synovial fluid of mild and severe temporomandibular joint internal derangements

Leonardi¹,

Perrotta²,

Almeida³

et al. 2016

Med Oral

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BackgroundTo understand the molecular basis of temporomandibular joint (TMJ) pathologies, we aimed to investigate the lubricin levels in the TMJ synovial fluid (SF) of patients with mild to severe internal derangements (IDs).Material and MethodsA total, 34 joints were the study group. Only patients, with a Wilkes stage of III, IV and V were included, in this sample. Control group consisted of SF from eight joints, from patients undergoing to orthognatic surgery. Concentrations of lubricin in the SF from both samples were measured using ELISA system.ResultsThe mean lubricin concentration was 7.029 ± 0.21 µg/mL in stage III patients; 5.64 ± 0.10 µg/mL in stage IV patients, and 4.78 ± 0.11 µg/mL in stage V patients. The lubricin levels from stage IV and stage V patients differed significantly (P ≤ 0.001) from those of control subjects. Lubricin levels were inversely correlated with age and to VAS score.ConclusionsThe results of this cross-sectional study highlight the relationship between disease severity and the levels of lubricin in TMJ SF. Our findings suggest that novel biotherapeutic approaches, including the administration of recombinant lubricin in the joint cavity, for the treatment of TMJ diseases can be developed. Key words:Lubricin, TMJ, derangements, synovial fluid.

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“…Lubricin has been associated with pathological conditions in the joint tissue, highlighted by the fact that the mutations of the Prg4 gene is associated with the camptodactyly-arthropathycoxa vara-pericarditis syndrome (CACP) 4 , a rare arthritis like autosomal recessive disorder causing joint abnormalities. The lubrication properties of lubricin also suggest that the protein is associated with arthritic diseases, since it has been proposed that defect lubrication should aggravate joint degradation 5 . Osteoarthritis (OA) is the dominating arthritic disease with high prevalence in elderly people and involves cartilage degradation in the articular joints, leading to pain and restricted motion 6 .…”

Section: Introductionmentioning

confidence: 99%

Core-2O-glycans are required for galectin-3 interaction with the osteoarthritis related protein lubricin

Ali

et al. 2019

Preprint

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Author contributions: SA planned and executed the glycomic analysis, KT and LA planned and executed the glycoproteomic analysis, SH performed ELISA analysis, SR performed SPR analysis, YM and JH designed the recombinant expression and experiments executed by YM. OR, LB, RK, TS and TE were responsible for control and patient sample collection. TS and GJ contributed with design and provision of rhPRG4 for the analysis, MS and AK participated in planning and supervision of galectin-3 experiments. SF, KT and NK performed the writing with input from all contributors. NK coordinated and directed the research. All authors signed off on the final version.ABSTRACT Synovial fluid lubricin (proteoglycan 4) is a mucin-type O-linked glycosylated (60% of the mass) biological lubricant involved in osteoarthritis (OA) development. Lubricin has been reported to be cross-linked by synovial galectin-3 on the lubricating articular surface. Here, we confirm that binding to galectin-3 depended on core-2 O-linked glycans, where surface plasmon resonance of a recombinant lubricin (rhPRG4) devoid of core-2 structures lacked binding capacity to recombinant galectin-3. Both galectin-3 levels and interactions with synovial lubricin were found to be decreased in late-stage OA patients coinciding with an increase of truncated and less sialylated core 1 O-glycans. These data suggest a defect cross-linking of surface active molecules in OA and provides novel insights into OA molecular pathology.

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Effects of Enzymatic Degradation after Loading in Temporomandibular Joint

Cited by 45 publications

References 31 publications

High-Frequency Near-Infrared Diode Laser Irradiation Attenuates IL-1β-Induced Expression of Inflammatory Cytokines and Matrix Metalloproteinases in Human Primary Chondrocytes

High-Frequency Near-Infrared Diode Laser Irradiation Attenuates IL-1β-Induced Expression of Inflammatory Cytokines and Matrix Metalloproteinases in Human Primary Chondrocytes

Lubricin in synovial fluid of mild and severe temporomandibular joint internal derangements

Core-2O-glycans are required for galectin-3 interaction with the osteoarthritis related protein lubricin

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