High-Frequency Near-Infrared Diode Laser Irradiation Attenuates IL-1β-Induced Expression of Inflammatory Cytokines and Matrix Metalloproteinases in Human Primary Chondrocytes

Sakata, Shuzo; Kunimatsu, Ryo; Tsuka, Yuji; Nakatani, Ayaka; Hiraki, Tomoka; Gunji, Hidemi; Hirose, Naoto; Yanoshita, Makoto; Putranti, Nurul Aisyah Rizky; Tanimoto, Kotaro

doi:10.3390/jcm9030881

Cited by 13 publications

(19 citation statements)

References 83 publications

(95 reference statements)

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“…In a rat model of wound healing, collagen deposition did not increase 3 days after laser treatment, but it increased significantly at day 7 after treatment [ 77 ]. Sakata et al [ 28 ] found that, in chondrocytes stimulated with IL-1β, MMP-1 increased and then decreased after NIR irradiation applied post-stimulation, in complete agreement with what has been observed in the present study on fibroblasts activated by IL-1β and TNF-α.…”

Section: Discussionsupporting

confidence: 92%

“…The cytokines IL-1β and TNF-α have already been used at different concentrations, individually or in association, to stimulate an inflammatory response in various cell types, dermal fibroblasts included [ 28 , 38 , 39 ]. In this study, IL-1β and TNF-α have been used jointly to induce a pro-inflammatory phenotype in dermal fibroblasts, with the aim to investigate if PBMT delivered via a dual-wavelength NIR laser system (MLS-MiS) was effective in counteracting cell inflammatory response and modulating fibroblast functions involved in stromal activation, wound healing, and its alterations, which can lead to chronic ulcers or fibrosis.…”

Section: Discussionmentioning

confidence: 99%

“…Another PBMT effect, which can be relevant in the evolution and outcome of the inflammatory response, is to induce a decrease in matrix metalloproteinases (MMPs) expression, as it has been recently demonstrated in an in vitro model of osteoarthritis [ 28 ]. MMPs are an important family of proteinases, able to degrade extracellular matrix components and covering a broad range of tasks in inflammation, acquired immunity, defense from injury and repair.…”

Section: Introductionmentioning

confidence: 99%

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Effect of NIR Laser Therapy by MLS-MiS Source on Fibroblast Activation by Inflammatory Cytokines in Relation to Wound Healing

et al. 2021

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The fine control of inflammation following injury avoids fibrotic scars or impaired wounds. Due to side effects by anti-inflammatory drugs, the research is continuously active to define alternative therapies. Among them, physical countermeasures such as photobiomodulation therapy (PBMT) are considered effective and safe. To study the cellular and molecular events associated with the anti-inflammatory activity of PBMT by a dual-wavelength NIR laser source, human dermal fibroblasts were exposed to a mix of inflammatory cytokines (IL-1β and TNF-α) followed by laser treatment once a day for three days. Inducible inflammatory key enzymatic pathways, as iNOS and COX-2/mPGES-1/PGE2, were upregulated by the cytokine mix while PBMT reverted their levels and activities. The same behavior was observed with the proangiogenic factor vascular endothelial growth factor (VEGF), involved in neovascularization of granulation tissue. From a molecular point of view, PBMT retained NF-kB cytoplasmatic localization. According to a change in cell morphology, differences in expression and distribution of fundamental cytoskeletal proteins were observed following treatments. Tubulin, F-actin, and α-SMA changed their organization upon cytokine stimulation, while PBMT reestablished the basal localization. Cytoskeletal rearrangements occurring after inflammatory stimuli were correlated with reorganization of membrane α5β1 and fibronectin network as well as with their upregulation, while PBMT induced significant downregulation. Similar changes were observed for collagen I and the gelatinolytic enzyme MMP-1. In conclusion, the present study demonstrates that the proposed NIR laser therapy is effective in controlling fibroblast activation induced by IL-1β and TNF-α, likely responsible for a deleterious effect of persistent inflammation.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of NIR Laser Therapy by MLS-MiS Source on Fibroblast Activation by Inflammatory Cytokines in Relation to Wound Healing

et al. 2021

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“…Excessive mechanical stress may alter the metabolism of mandibular condylar chondrocyte and decrease the amount of extracellular matrix (Fujita et al, 2019; Kuroda et al, 2009; Ogasawara et al, 2020). In articular cartilage affected by OA, reduction of the amounts of extracellular matrix such as type II collagen and aggrecan (ACAN) is caused by the decrease in protein synthesis from chondrocytes, and the activation of extracellular matrix degrading enzymes including matrix metalloproteinases (MMPs) and a disintegrin and MMP with thrombospondin motifs, which are major cartilage aggrecanases in humans (Curtin & Reville, 1995; Sakata et al, 2020). The reduction of ACAN through aggrecanase enzyme activity is a key step in early OA (Huang & Wu, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…The reduction of ACAN through aggrecanase enzyme activity is a key step in early OA (Huang & Wu, 2008). MMPs, and especially MMP‐13, are fatal enzymes associated with the degradation of articular cartilage in OA (Sakata et al, 2020; Sekino et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

The immunoregulatory role of p21 in the development of the temporomandibular joint‐osteoarthritis

Khurel‐Ochir

Izawa

Iwasa

et al. 2021

Clinical & Exp Dental Res

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Objective We aimed to identify the immunoregulatory role of the cyclin‐dependent kinase inhibitor p21 in the homeostasis of mandibular condylar cartilage affected by mechanical stress. Materials and methods Ten C57BL/6 wild‐type (WT) and ten p21−/− mice aged 8 weeks were divided into the untreated and treated groups. In the treated groups, mechanical stress was applied to the temporomandibular joint (TMJ) through forced mouth opening for 3 hr/day for 7 days. The dissected TMJs were assessed using micro‐CT, histology, and immunohistochemistry. Results Treated p21−/− condyles with mechanical stress revealed more severe subchondral bone destruction, with thinner cartilage layers and smaller proteoglycan area relative to treated WT condyles; untreated WT and p21−/− condyles had smoother surfaces. Immunohistochemistry revealed significant increases in the numbers of caspase‐3, interleukin‐1β, matrix metalloprotease (MMP)‐9, and MMP‐13 positive cells, and few aggrecan positive cells, in treated p21−/− than in treated WT samples. Moreover, the number of TUNEL positive cells markedly increased in p21−/− condyles affected by mechanical stress. Conclusions Our findings indicate that p21 in chondrocytes contributes to regulate matrix synthesis via the control ofm aggrecan and MMP‐13 expression under mechanical stress. Thus, p21 might regulate the pathogenesis of osteoarthritis in the TMJ.

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High-frequency near-infrared diode laser irradiation suppresses IL-1β-induced inflammatory cytokine expression and NF-κB signaling pathways in human primary chondrocytes

et al. 2021

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High-Frequency Near-Infrared Diode Laser Irradiation Attenuates IL-1β-Induced Expression of Inflammatory Cytokines and Matrix Metalloproteinases in Human Primary Chondrocytes

Cited by 13 publications

References 83 publications

Effect of NIR Laser Therapy by MLS-MiS Source on Fibroblast Activation by Inflammatory Cytokines in Relation to Wound Healing

Effect of NIR Laser Therapy by MLS-MiS Source on Fibroblast Activation by Inflammatory Cytokines in Relation to Wound Healing

The immunoregulatory role of p21 in the development of the temporomandibular joint‐osteoarthritis

High-frequency near-infrared diode laser irradiation suppresses IL-1β-induced inflammatory cytokine expression and NF-κB signaling pathways in human primary chondrocytes

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