Effects of environmental factors on low-molecular-weight peptides of fishes: a review

Смирнов, Л. П.; Sukhovskaya, Irina; Немова, Н. Н.

doi:10.1007/s11184-005-0007-0

Cited by 13 publications

(9 citation statements)

References 58 publications

(63 reference statements)

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“…MT fraction from control fish brain also scavenged superoxide radicals to some extent. Although we could not fully characterize the molecular structure of isolated MT, the electrophoretic analysis suggests it to be a low molecular weight protein like other fish MTs [27]. Hao et al [2] also reported the induction of a low molecular weight (6.7 kDa) MT in the brain of rainbow trout.…”

Section: Discussionmentioning

confidence: 68%

Channa punctata brain metallothionein is a potent scavenger of superoxide radicals and prevents hydroxyl radical‐induced in vitro DNA damage

Atif

Kaur

Ansari

et al. 2008

J Biochem & Molecular Tox

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Mammalian brain metallothioneins (MTs) have been shown to scavenge free radicals. However, a similar role for fish brain MT has not been established yet. Previously, we have reported that MT from the liver of a freshwater fish, Channa punctata Bloch, had free-radical-scavenging activity in vitro. In this study, we report on the induction of MT in brain and other tissues of C. punctata treated with a low concentration of zinc chloride. We partially purified MT (Zn-MT)-rich fraction from the brain and studied its free-radical-scavenging and DNA damage attenuating effects. Zinc exposure showed significant MT induction in brain, gill, kidney, and liver. C. punctata brain MT efficiently scavenged superoxide radicals and also attenuated hydroxyl radical-mediated DNA damage. These findings suggest that fish brain MT has a free-radical-scavenging activity, and its expression may be regulated in response to stress and chemical exposure. C. punctata has been identified as a potent biomarker fish species. It is suggested that this fish species may be a good model for the study of MTs with regard to their regulatory and biomarker functions.

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Section: Discussionmentioning

confidence: 68%

Channa punctata brain metallothionein is a potent scavenger of superoxide radicals and prevents hydroxyl radical‐induced in vitro DNA damage

Atif

Kaur

Ansari

et al. 2008

J Biochem & Molecular Tox

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“…changed along the metal contamination gradient (Figure 2; Supplemental Data, Figure S3) but with opposite trends; for Co the relative contribution of the medium-molecular weight pool increased slightly in importance as the total cytosolic concentration increased, whereas for Ni the contribution of the medium-molecular weight pool decreased markedly as the cytosolic Ni concentration increased (Supplemental Data, Figure S3). This observation is of interest because metallothioneins and metallothionein-like peptides and proteins are found in the medium-molecular weight fraction with a molecular weight ranging from 9 to 20 kDa [22,23] and therefore do not seem, in the present study, to be involved in Ni detoxification. This aspect will be discussed in the next section Metal binding to metallothionein-like peptides and other thermostable ligands.…”

Section: Metal Binding To Cytosolic Ligands (Whole Cytosol)mentioning

confidence: 88%

Binding of trace elements (Ag, Cd, Co, Cu, Ni, and Tl) to cytosolic biomolecules in livers of juvenile yellow perch (Perca flavescens) collected from lakes representing metal contamination gradients

Caron

Rosabal

Drevet

et al. 2017

Enviro Toxic and Chemistry

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Biomolecules involved in handling cytosolic metals in the liver of the yellow perch (Perca flavescens) were characterized in juvenile fish collected from 4 lakes constituting metal contamination gradients. Using size-exclusion liquid chromatography coupled to an inductively coupled mass spectrometer, we determined metal distributions among ligands of different molecular weights in the cytosol, before and after a heat denaturation step designed to isolate metallothionein-like peptides and proteins. Silver, Cd, and Cu found in the heat-stable protein supernatants were indeed largely present as metallothionein-like peptide complexes; but Co, Ni, and Tl, also present in the heat-stable protein supernatants, did not coelute with metallothionein-like peptides and proteins. This difference in metal partitioning is consistent with the known preference of "soft" metals such as Ag, Cd, and Cu(I) for thiolated ligands and the contrasting tendency of Co and Ni to bind to ligands with oxygen and nitrogen as donor atoms. Metal handling in the whole cytosol also reflected these differences in metal-binding behavior. For Cd and Cu, the importance of the molecular weight pool that includes metallothionein-like peptides and proteins increased relative to the other pools as the total cytosolic metal concentration ([M] ) increased, consistent with a concentration-dependent detoxification response. In contrast, for Ni and Tl the increase in [M] was accompanied by a marked increase in the high-molecular weight (670-33 kDa) pool, suggesting that hepatic Ni and Tl are not effectively detoxified. Overall, the results suggest that metal detoxification is less effective for Ni, Tl, and Co than for Ag, Cd, and Cu. Environ Toxicol Chem 2018;37:576-586. © 2017 SETAC.

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“…This latter protein is very important in the detoxification of toxic metals through the dismutation of zinc-binding by, for example, Cd and Cu (although at lower levels, Cu also has a significant importance in MT metal-biding). In contrast to Zn, Cu and Cd, there is no evidence that Pb produces the metal-binding protein metallothionein (SMIRNOV et al, 2005).…”

Section: Introductionmentioning

confidence: 94%

Accumulation and distribution of metals in the tissues of two catfish species from Cananéia and Santos-São Vicente estuaries

2012

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A B S T R A C TPb, Cd, Zn, and Cu concentrations and their distribution in liver, gill, and muscle tissues of the catfish Cathorops spixii and Genidens genidens were investigated in order to provide information concerning the bioaccumulation processes in these species. Fish were collected in one estuary subject to slight human influence (Cananéia) and in two regions within the Santos-São Vicente estuary, São Paulo, Brazil, subject to distinct anthropogenic influences: 1) industrial and 2) domestic. In general, the highest concentrations of Cu, Zn, Cd, and Pb were found in the liver tissue. Muscle showed the lowest values of metal accumulation for all the elements investigated. Regarding the distribution and the metal pollution index (MPI), the levels of the metals analyzed decreased in the following order: liver>gill>muscle. MPI results suggest that gill and liver are important organs to exemplify the bioaccumulation process and are also better able to reflect both recent and chronic exposure to metals. R E S U M O Concentrações de Pb, Cd, Zn e Cu e sua distribuição no fígado, brânquias e músculo dos bagresCathorops spixii e Genidens genidens foram determinadas visando fornecer informações sobre os processos de bioacumulação desses metais nas espécies. Os peixes foram coletados no estuário de Cananéia e em duas regiões do estuário de Santos-São Vicente, ambos em São Paulo, Brasil. Esses estuários apresentam distintas influências antropogênicas, sendo o primeiro deles submetido a impacto industrial e o segundo a impacto doméstico. Em geral, as maiores concentrações de Cu, Zn, Cd e Pb foram obtidas no tecido hepático. No músculo foram encontrados os menores valores de acumulação para todos os elementos investigados. Quanto à distribuição dos metais e ao seu índice de poluição (Índice de Poluição por Metais-IPM), os níveis diminuíram na seguinte ordem: fígado>brânquias>músculo. Resultados do IPM sugerem que as brânquias e o fígado são importantes órgãos para indicar processos de bioacumulação, sendo também os melhores para refletir exposições crônica e recente a metais.

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Effects of environmental factors on low-molecular-weight peptides of fishes: a review

Cited by 13 publications

References 58 publications

Channa punctata brain metallothionein is a potent scavenger of superoxide radicals and prevents hydroxyl radical‐induced in vitro DNA damage

Channa punctata brain metallothionein is a potent scavenger of superoxide radicals and prevents hydroxyl radical‐induced in vitro DNA damage

Binding of trace elements (Ag, Cd, Co, Cu, Ni, and Tl) to cytosolic biomolecules in livers of juvenile yellow perch (Perca flavescens) collected from lakes representing metal contamination gradients

Accumulation and distribution of metals in the tissues of two catfish species from Cananéia and Santos-São Vicente estuaries

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