Effects of Endurance and Resistance Training on Calcitonin Gene-Related Peptide and Acetylcholine Receptor at Slow and Fast Twitch Skeletal Muscles and Sciatic Nerve in Male Wistar Rats

Abstract

The aim of this study was to investigate effects of endurance and resistance training (ET and RT) on CGRP and AChRs at slow and fast twitch muscles and sciatic nerve in rats. Twenty-five male rats were randomly assigned into three groups including sedentary (SED), endurance training (ET), and resistance training (RT). Animals of ET exercised for 12 weeks, five times/week, and 60 min/day at 30 m/min. Animals of RT were housed in metal cage with 2 m high wire-mesh tower, with water bottles set at the top. 48 h after the last session of training protocol, animals were anaesthetized. The right sciatic nerves were removed; then, Soleus (SOL) and Tibialis anterior (TA) muscles were excised and immediately snap frozen in liquid nitrogen. All frozen tissues were stored at −80°C. Results showed that, after both ET and RT, CGRP content as well as AChR content of SOL and TA muscles significantly increased. But there was no significant difference among groups at sciatic nerve' CGRP content. In conclusion, data demonstrate that ET and RT lead to changes of CGRP and AChR content of ST and FT muscles. The changes indicate to the importance of neuromuscular activity.

Effects of exercise on neuromuscular junction components across age: systematic review of animal experimental studies

“…In the peripheral nervous system, CGRP increases the expression of synaptic AChR by increasing the opening time of it channels [ 33 , 34 ]. Parnow et al [ 35 ] studied the effects of endurance training (60 min/day at 30 m/min) and resistance (2 m wire mesh tower) on CGRP and AChR slow-twitch (soleus) and fast-twitch (Tibialis anterior) muscle fibers and Sciatic nerve. The results showed not to differ between muscles of different features and that both types of training increased significantly CGRP and AChR.…”

The Neuropeptide α-Calcitonin Gene-Related Peptide as the Mediator of Beneficial Effects of Exercise in the Cardiovascular System

“…The major fraction of circulating immunoreactive αCGRP comprises the intact neuropeptide, suggesting exercise-induced αCGRP production and excluding the possibility that the increase in concentrations of immunoreactive αCGRP detected during exercise is caused by molecule degradation products ( Schifter, 1991 ; Schifter et al, 1995 ; Hasbak et al, 2002 ). Both endurance and resistance training are capable of increasing αCGRP content in skeletal muscle tissues ( Parnow et al, 2012 ) and increased αCGRP content is detectable in motor neurons and skeletal muscle tissues even 2 days after exercise ( Homonko and Theriault, 1997 ).…”

Φυσικοθεραπευτική Αξιολόγηση Και Αποκατάσταση Σε Κακώσεις Του Βραχιονίου Πλέγματος

“…(74,75) Ειδικότερα, η επίδραση της άσκησης αναφέρεται στην ανάδρομη ερεθιστική δράση στον κινητικού νευρώνα, στην αύξηση του αριθμού των νευραξόνων που αναγεννιούνται και στον ρυθμό της επιμήκυνσης των νευραξόνων και των διακλαδώσεων τους. (71,74,74,125,126) Επίσης, αναφέρεται πως η ενεργητική άσκηση δρα στο επίπεδο βλάβης βελτιώνοντας το περιβάλλον της νευρικής αναγέννησης, επιδρώντας στα κύτταρα Schwann. (78,127) Στην μελέτη μας βρήκαμε επίσης, πως σε ασθενείς που χρησιμοποιήθηκε το S.E.M.G Biofeedback είχαν μεγαλύτερη μυϊκή ισχύ όλων των μυϊκών ομάδων που εμπλέκονται στη βλάβη σε σχέση με τους υπόλοιπους.…”