Effects of eCG and FSH on ovarian response, recovery rate and number and quality of oocytes obtained by ovum pick-up in Holstein cows

Şendağ, Sait; Çetin, Yunus; Alan, Muhammet; Hadeler, Klaus‐Gerd; Niemann, Heiner

doi:10.1016/j.anireprosci.2008.01.007

Cited by 41 publications

(20 citation statements)

References 26 publications

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“…Superstimulation with FSH previous to OPU has been used successfully for IVP programs in cattle, resulting in increased total embryo yields per OPU session (Goodhand et al, 1999;Sendag et al, 2008;Vieira et al, 2014), possibly because of the greater follicular diameters of the aspirated follicles. The FSH treatment for superstimulation can promote the growth of a homogeneous follicle population and to recover competent oocytes suitable for IVEP procedures.…”

Section: Superstimulation With Fsh Prior To Ovum Pick-upmentioning

confidence: 99%

Assisted reproductive technologies (ART) in water buffaloes

Baruselli¹,

Soares²,

Bayeux³

et al. 2018

Anim Reprod

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Our expanding knowledge of ovarian function during the buffalo estrous cycle has given new approaches for the precise synchronization of follicular development and ovulation to apply consistently assisted reproductive technologies (ART). Recent synchronization protocols are designed to control both luteal and follicular function and permit fixed-time AI with high pregnancy rates during the breeding (autumn-winter) and nonbreeding (springsummer) seasons. Additionally, allow the initiation of superstimulatory treatments at a self-appointed time and provide opportunities to do fixed-time AI in donors and fixed-time embryo transfer in recipients. However, due the scarce results of in vivo embryo recovery in superovulated buffaloes, the association of ovum pick-up (OPU) with in vitro embryo production (IVEP) represents an alternative method of exploiting the genetics of high yeld buffaloes. Nevertheless, several factors appear to be critical to OPU/IVEP efficiency, including antral follicle population, follicular diameter, environment, farm and category of donor. This review discusses a number of key points related to the manipulation of ovarian follicular growth to improve assisted reproductive technologies in buffalo.

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Section: Superstimulation With Fsh Prior To Ovum Pick-upmentioning

confidence: 99%

Assisted reproductive technologies (ART) in water buffaloes

Baruselli¹,

Soares²,

Bayeux³

et al. 2018

Anim Reprod

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“…Among the strategies used, ovarian antral follicle counts in the donors, evaluated directly by ultrasonography [10][11][12][13][14] or indirectly by the concentrations of anti-Mullerian hormone [7,[15][16][17][18] are beginning to be applied in the field. Another alternative is the use of follicular wave synchronization protocols associated with superstimulation treatments before the OPU [19][20][21]. Earlier studies reported associations between IVP outcomes and the stage of follicular growth at which OPU is performed [22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Efficacy of a single intramuscular injection of porcine FSH in hyaluronan prior to ovum pick-up in Holstein cattle

Vieira¹,

Rodrigues

Netto

et al. 2016

Theriogenology

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Plasma FSH profiles, in vitro embryo production (IVP) after ovum pickup (OPU), and establishment of pregnancy with IVP embryos were compared in untreated Holstein oocyte donors and those superstimulated with multiple injections or a single intramuscular (IM) injection of porcine FSH (pFSH) in hyaluronan (HA). Plasma FSH profiles were determined in 23 heifers randomly allocated to one of four groups. Controls received no treatment, whereas the F200 group received 200 mg of pFSH in four doses, 12 hours apart. The F200HA and F300HA groups received 200- or 300-mg pFSH in 5 mL or 7.5 mL, respectively of a 0.5% HA solution by a single IM injection. Plasma FSH levels were determined before the first pFSH treatment and every 6 hours over 96 hours. All data were analyzed by orthogonal contrasts. Circulating FSH area under curve (AUC) in pFSH-treated animals was greater than that in the control group (P = 0.02). Although the AUC did not differ among FSH-treated groups (P = 0.56), the total period with elevated plasma FSH was greater in the F200 group than in the HA groups (P < 0.0001). However, the F300HA group had a greater AUC than the F200HA group (P = 0.006), with a similar total period with elevated plasma FSH (P = 0.17). The IVP was performed in 90 nonlactating Holstein cows randomly allocated to one of the four treatment groups as in the first experiment. A greater proportion of medium-sized (6-10 mm) follicles was observed in cows receiving pFSH, regardless of the treatment group (P < 0.0001). Also, numbers of follicles (P = 0.01), cumulus-oocyte complexes (COCs) retrieved (P = 0.01) and matured (P = 0.02), cleavage rates (P = 0.002), and blastocysts produced per OPU session (P = 0.06) were greater in cows receiving pFSH, regardless of the treatment group. Cows in the F200HA group had a greater recovery rate (P = 0.009), number of COCs cultured (P = 0.04), and blastocysts produced per OPU session (P = 0.06) than cows in the F300HA group. Similar pregnancy rates were observed 50 to 60 days after transferring IVP embryos from donors in the different treatment groups (P > 0.05). In conclusion, a single IM injection of pFSH combined in 0.5% HA resulted in similar plasma FSH profiles as twice-daily pFSH treatments. Treatment of nonlactating donors with pFSH, with or without HA, resulted in increased IVP over untreated controls. A single dose of 200 mg of pFSH in 0.5% HA resulted in greater IVP than 300-mg pFSH in HA. Finally, pregnancy rates with IVP embryos were similar, regardless donor treatment.

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“…As diferenças nos resultados encontrados neste trabalho não foram significativas (p>0,05). Sendag et al (2008) encontraram melhores resultados utilizando 500 UI de FSH comparando com a aplicação de 3000UI de eCG, em vacas Holandesas. No trabalho os autores sugerem que a resposta ovariana, o número de folículo nos ovários e número oócitos, além de sua qualidade são afetados pelo tipo de gonadotrofina aplicada, sendo o FSH a melhor alternativa para OPU em relação ao eCG.…”

Section: Resultsunclassified