Effects of Drought Stress at Early Growth Stage on Response of Sugarcane to Different Nitrogen Application

Dinh, Hoang Thai; Watanable, Kenta; Takaragawa, Hiroo; Kawamitsu, Yoshinobu

doi:10.1007/s12355-017-0566-y

Cited by 14 publications

(10 citation statements)

References 29 publications

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“…In this study, we examined molecular regulation mechanisms of sugarcane in response to DS during the tillering stage. Responses to DS by sugarcane include leaf yellowing, leaf rolling, inhibition of stalk and leaf growth, and reduced biomass [ 18 ]. We observed similar phenotypes for Badila exposed to DS.…”

Section: Discussionmentioning

confidence: 99%

Global Responses of Autopolyploid Sugarcane Badila (Saccharum officinarum L.) to Drought Stress Based on Comparative Transcriptome and Metabolome Profiling

ShaoHua

Chu

Feng

et al. 2023

IJMS

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Sugarcane (Saccharum spp. hybrid) is frequently affected by seasonal drought, which causes substantial declines in quality and yield. To understand the drought resistance mechanisms of S. officinarum, the main species of modern sugarcane, at a molecular level, we carried out a comparative analysis of transcriptome and metabolome profiling of the sugarcane variety Badila under drought stress (DS). Compared with control group (CG) plants, plants exposed to DS had 13,744 (6663 up-regulated and 7081 down-regulated) differentially expressed genes (DEGs). GO and KEGG analysis showed that the DEGs were enriched in photosynthesis-related pathways and most DEGs had down-regulated expression. Moreover, the chlorophyll content, photosynthesis (Photo), stomatal conductance (Cond), intercellular carbon dioxide concentration (Ci) and transpiration rate (Trmmol) were sharply decreased under DS. These results indicate that DS has a significant negative influence on photosynthesis in sugarcane. Metabolome analysis identified 166 (37 down-regulated and 129 up-regulated) significantly regulated metabolites (SRMs). Over 50% of SRMs were alkaloids, amino acids and their derivatives, and lipids. The five most significantly enriched KEGG pathways among SRMs were Aminoacyl-tRNA biosynthesis, 2-Oxocarboxylic acid metabolism, Biosynthesis of amino acids, Phenylalanine metabolism, and Arginine and proline metabolism (p < 0.05). Comparing CG with DS for transcriptome and metabolome profiling (T_CG/DS and M_CG/DS, respectively), we found three of the same KEGG-enriched pathways, namely Biosynthesis of amino acids, Phenylalanine metabolism and Arginine and proline metabolism. The potential importance of Phenylalanine metabolism and Arginine and proline metabolism was further analyzed for response to DS in sugarcane. Seven SRMs (five up-regulated and two down-regulated) and 60 DEGs (17 up-regulated and 43 down-regulated) were enriched in Phenylalanine metabolism under DS, of which novel.31261, Sspon.04G0008060-1A, Sspon.04G0008060-2B and Sspon.04G0008060-3C were significantly correlated with 7 SRMs. In Arginine and proline metabolism, eight SRMs (seven up-regulated and one down-regulated) and 63 DEGs (32 up-regulated and 31 down-regulated) were enriched, of which Sspon.01G0026110-1A (OAT) and Sspon.03G0002750-3D (P5CS) were strongly associated with proline (r > 0.99). These findings present the dynamic changes and possible molecular mechanisms of Phenylalanine metabolism as well as Arginine and proline metabolism under DS and provide a foundation for future research and sugarcane improvement.

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Section: Discussionmentioning

confidence: 99%

Global Responses of Autopolyploid Sugarcane Badila (Saccharum officinarum L.) to Drought Stress Based on Comparative Transcriptome and Metabolome Profiling

ShaoHua

Chu

Feng

et al. 2023

IJMS

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“…Responses to DS by crops involve multiple processes, including perception, signal transduction, osmoprotection, transcription, translation, protein modification, and metabolic regulation [ 20 ]. In sugarcane, plant height, plant diameter, total leaf area, and dry matter accumulation were significantly reduced by DS [ 21 ]. In this study, we saw similar responses to DS, particularly reduced plant height and withered leaves, which are adaptive traits to protect against water loss.…”

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of Transcriptome and Metabolome Reveals the Regulation of Chitooligosaccharide on Drought Tolerance in Sugarcane (Saccharum spp. Hybrid) under Drought Stress

ShaoHua

Chu

Zhou

et al. 2022

IJMS

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Sugarcane (Saccharum spp. hybrid) is an important crop for sugar and biofuels, and often suffers from water shortages during growth. Currently, there is limited knowledge concerning the molecular mechanism involved in sugarcane response to drought stress (DS) and whether chitooligosaccharide could alleviate DS. Here, we carried out a combined transcriptome and metabolome of sugarcane in three different treatment groups: control group (CG), DS group, and DS + chitooligosaccharide group (COS). A total of 12,275 (6404 up-regulated and 5871 down-regulated) differentially expressed genes (DEGs) were identified when comparing the CG and DS transcriptomes (T_CG/DS), and 2525 (1261 up-regulated and 1264 down-regulated) DEGs were identified in comparing the DS and COS transcriptomes (T_DS/COS). GO and KEGG analysis showed that DEGs associated with photosynthesis were significantly enriched and had down-regulated expression. For T_DS/COS, photosynthesis DEGs were also significantly enriched but had up-regulated expression. Together, these results indicate that DS of sugarcane has a significantly negative influence on photosynthesis, and that COS can alleviate these negative effects. In metabolome analysis, lipids, others, amino acids and derivatives and alkaloids were the main significantly different metabolites (SDMs) observed in sugarcane response to DS, and COS treatment reduced the content of these metabolites. KEGG analysis of the metabolome showed that 2-oxocarboxylic acid metabolism, ABC transporters, biosynthesis of amino acids, glucosinolate biosynthesis and valine, leucine and isoleucine biosynthesis were the top-5 KEGG enriched pathways when comparing the CG and DS metabolome (M_CG/DS). Comparing DS with COS (M_DS/COS) showed that purine metabolism and phenylalanine metabolism were enriched. Combined transcriptome and metabolome analysis revealed that pyruvate and phenylalanine metabolism were KEGG-enriched pathways for CG/DS and DS/COS, respectively. For pyruvate metabolism, 87 DEGs (47 up-regulated and 40 down-regulated) and five SDMs (1 up-regulated and 4 down-regulated) were enriched. Pyruvate was closely related with 14 DEGs (|r| > 0.99) after Pearson’s correlation analysis, and only 1 DEG (Sspon.02G0043670-1B) was positively correlated. For phenylalanine metabolism, 13 DEGs (7 up-regulated and 6 down-regulated) and 6 SDMs (1 up-regulated and 5 down-regulated) were identified. Five PAL genes were closely related with 6 SDMs through Pearson’s correlation analysis, and the novel.31257 gene had significantly up-regulated expression. Collectively, our results showed that DS has significant adverse effects on the physiology, transcriptome, and metabolome of sugarcane, particularly genes involved in photosynthesis. We further show that COS treatment can alleviate these negative effects.

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“…Bastos et al (2015) evaluated the effect of irrigation water depths and nitrogen rates on the sugarcane yield and dry biomass accumulation and found that the use of 100 kg ha -1 of urea increased the sugarcane culm production in 20.85 Mg ha -1 , which is due to increases in dry biomass production. Dinh et al (2018) reported that the dry culm biomass of sugarcane plants grown under increasing nitrogen rates up to 180 kg ha -1 tends to increase, as much as the nitrogen available, and emphasized that water stress affects the use of the nutrient by the plant and, consequently, the crop yield, with higher yields for plants grown under no water deficit.…”

Section: Discussionmentioning

confidence: 99%

Water depths and nitrogen rates on sugarcane growth and dry biomass accumulation

Costa¹,

Costa

Rolim

et al. 2022

Biosci. J.

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Irrigation and soil fertilization management are essential agricultural practices that improve the growth and development of sugarcane plants and, consequently, increase their production capacity, which is important for sugar and alcohol productions. In this context, the objective of this work was to evaluate the effect of water depths and nitrogen rates on the growth and dry biomass accumulation of sugarcane plants. The treatments consisted in four water depths (1,498; 1,614; 1,739; and 1,854 mm), five nitrogen rates (0; 20; 40; 80; and 120 kg ha-1) and five evaluation times. The experiment was conducted in a randomized block design with a split-split-plot arrangement and four replications, including the factors water depths, nitrogen rates, and days after planting. The dry biomasses of the plant pointer, leaves and culms, culm diameter, plant height, and number of plants were analyzed. The application of nitrogen increased the sugarcane biomass, mainly the pointer (with leaves) and dry culm biomass, and the number of plants. The highest dry culm biomass accumulation and dry leaf biomass were found at the end of the crop cycle for the treatment with the application of nitrogen rates of 80 and 120 kg ha-1. The increases in water depths applied increased the number of plants per linear meter, but the culm and dry leaf biomass did not happen.

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Effects of Drought Stress at Early Growth Stage on Response of Sugarcane to Different Nitrogen Application

Cited by 14 publications

References 29 publications

Global Responses of Autopolyploid Sugarcane Badila (Saccharum officinarum L.) to Drought Stress Based on Comparative Transcriptome and Metabolome Profiling

Global Responses of Autopolyploid Sugarcane Badila (Saccharum officinarum L.) to Drought Stress Based on Comparative Transcriptome and Metabolome Profiling

Integrated Analysis of Transcriptome and Metabolome Reveals the Regulation of Chitooligosaccharide on Drought Tolerance in Sugarcane (Saccharum spp. Hybrid) under Drought Stress

Water depths and nitrogen rates on sugarcane growth and dry biomass accumulation

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