Integrated Analysis of Transcriptome and Metabolome Reveals the Regulation of Chitooligosaccharide on Drought Tolerance in Sugarcane (Saccharum spp. Hybrid) under Drought Stress

ShaoHua, Yang; Chu, Na; Zhou, Hongkai; Li, Jiashuo; Feng, Naijie; Su, Junbo; Deng, Zuhu; Shen, Xuefeng; Dian-feng, Zheng

doi:10.3390/ijms23179737

Cited by 12 publications

(9 citation statements)

References 58 publications

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“…2-oxocarboxylic acids connect various amino acid metabolism/ synthesis pathways, including the tricarboxylic acid cycle, and play an important role in plant drought resistance. Yang et al (2022), who examined sugarcane response to drought, reported that 2oxocarboxylic acid metabolism was the most important enrichment pathway. In this study, 2-oxocarboxylic acid metabolism was found to play an essential role in both soybean varieties.…”

Section: Discussionmentioning

confidence: 99%

Regulation of soybean drought response by mepiquat chloride pretreatment

Wang

Zhou

et al. 2023

Front. Plant Sci.

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IntroductionSoybean is the world’s most important cultivated crop, and drought can affect their growth and, eventually, yields. Foliar application of mepiquat chloride (MC) can potentially alleviate the damage caused by drought stress in plants; however, the mechanism of MC regulation of soybean drought response has not been studied.MethodsThis study investigated the mechanism of soybean drought response regulation by mepiquat chloride in two varieties of soybean, sensitive Heinong 65 (HN65) and drought-tolerant Heinong44 (HN44), under three treatment scenarios, normal, drought stress, and drought stress + MC conditions.Results and discussionMC promoted dry matter accumulation under drought stress, reduced plant height, decreased antioxidant enzyme activity, and significantly decreased malondialdehyde content. The light capture processes, photosystems I and II, were inhibited; however, accumulation and upregulation of several amino acids and flavonoids by MC was observed. Multi-omics joint analysis indicated 2-oxocarboxylic acid metabolism and isoflavone biosynthetic pathways to be the core pathways by which MC regulated soybean drought response. Candidate genes such as LOC100816177, SOMT-2, LOC100784120, LOC100797504, LOC100794610, and LOC100819853 were identified to be crucial for the drought resistance of soybeans. Finally, a model was constructed to systematically describe the regulatory mechanism of MC application in soybean under drought stress. This study fills the research gap of MC in the field of soybean resistance.ConclusionNETs can promote gastric cancer metastasis by initiating COX-2 through TLR2, and COX-2 may become a target for gastric cancer immunotherapy.

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Section: Discussionmentioning

confidence: 99%

Regulation of soybean drought response by mepiquat chloride pretreatment

Wang

Zhou

et al. 2023

Front. Plant Sci.

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“…In this study, based on the DEGs between F172 and GT31 under drought stress, the results of the KEGG pathway analysis showed that starch and sucrose metabolism, and glycolysis/gluconeogenesis were extensively enriched for all comparisons ( Figures 2B, C ). Changes in these two metabolic pathways may be some of the most common responses to drought because these pathways are also significantly enriched for the IACSP97-7065, IACSP94-2094, and ROC22 sugarcane varieties under drought stress ( Contiliani et al., 2022 ; Yang et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…(2018) reported that drought inhibits photosynthesis; further, MAPK cascades modulate plant tolerance to drought ( Bashir et al., 2019 ). Drought can also affect the levels of secondary plant metabolites such as calcium, anthocyanins, flavonoids, phenolic acids, chlorophyll and saponins ( Yu et al., 2020 ; Contiliani et al., 2022 ; Yang et al., 2022 ). Wan et al.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptomic analyses of two sugarcane Saccharum L. cultivars differing in drought tolerance

Li,

Gui,

Zhu

et al. 2023

Front. Plant Sci.

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Sugarcane (Saccharum spp.) is an important cash crop, and drought is an important factors limiting its yield. To study the drought resistance mechanism of sugarcane, the transcriptomes of two sugarcane varieties with different levels of drought resistance were compared under different water shortage levels. The results showed that the transcriptomes of the two varieties were significantly different. The differentially expressed genes were enriched in starch and sucrose metabolism, linoleic acid metabolism, glycolysis/gluconeogenesis, and glyoxylate and dicarboxylate metabolic pathways. Unique trend genes of the variety with strong drought resistance (F172) were significantly enriched in photosynthesis, mitogen-activated protein kinases signaling pathway, biosynthesis of various plant secondary metabolites, and cyanoamino acid metabolism pathways. Weighted correlation network analysis indicated that the blue4 and plum1 modules correlated with drought conditions, whereas the tan and salmon4 modules correlated with variety. The unique trend genes expressed in F172 and mapped to the blue4 module were enriched in photosynthesis, purine metabolism, starch and sucrose metabolism, beta-alanine metabolism, photosynthesis-antenna proteins, and plant hormone signal transduction pathways. The expression of genes involved in the photosynthesis-antenna protein and photosynthesis pathways decreased in response to water deficit, indicating that reducing photosynthesis might be a means for sugarcane to respond to drought stress. The results of this study provide insights into drought resistance mechanisms in plants, and the related genes and metabolic pathways identified may be helpful for sugarcane breeding in the future.

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“…The functions of the DEMs enriched pathways were studied using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database ( https://www.genome.jp/kegg/pathway.html ). When the metabolic pathway p value was less than 0.05, the metabolic pathway was considered statistically significant enrichment [ 18 , 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…html). When the metabolic pathway p value was less than 0.05, the metabolic pathway was considered statistically significant enrichment [18,21].…”

Section: Metabolite Extraction and Untargeted Metabolomic Analysismentioning

confidence: 99%

Transcriptomics and metabolomics analysis reveal the dietary copper deficiency and supplementation effects of liver gene expression and metabolite change in grazing sheep

Jin,

Meng,

et al. 2024

BMC Genomics

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Background The appropriate mineral nutrients are essential for sheep growth and reproduction. However, traditional grazing sheep often experience mineral nutrient deficiencies, especially copper (Cu), due to inadequate mineral nutrients from natural pastures. Results The results indicated that dietary Cu deficiency and supplementation significantly reduced and elevated liver concentration of Cu, respectively (p < 0.05). FOXO3, PLIN1, ACTN2, and GHRHR were identified as critical genes using the weighted gene co-expression network analysis (WGCNA), quantitative real-time polymerase chain reaction (qRT-PCR), and receiver operating characteristic curve (ROC) validation as potential biomarkers for evaluating Cu status in grazing sheep. Combining these critical genes with gene functional enrichment analysis, it was observed that dietary Cu deficiency may impair liver regeneration and compromise ribosomal function. Conversely, dietary Cu supplementation may enhance ribosomal function, promote lipid accumulation, and stimulate growth and metabolism in grazing sheep. Metabolomics analysis indicated that dietary Cu deficiency significantly decreased the abundance of metabolites such as cholic acid (p < 0.05). On the other hand, dietary Cu supplementation significantly increased the abundance of metabolites such as palmitic acid (p < 0.05). Integrative analysis of the transcriptome and metabolome revealed that dietary Cu deficiency may reduce liver lipid metabolism while Cu supplementation may elevate it in grazing sheep. Conclusions The Cu content in diets may have an impact on hepatic lipid metabolism in grazing sheep. These findings provide new insights into the consequences of dietary Cu deficiency and supplementation on sheep liver and can provide valuable guidance for herders to rationalize the use of mineral supplements.

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Integrated Analysis of Transcriptome and Metabolome Reveals the Regulation of Chitooligosaccharide on Drought Tolerance in Sugarcane (Saccharum spp. Hybrid) under Drought Stress

Cited by 12 publications

References 58 publications

Regulation of soybean drought response by mepiquat chloride pretreatment

Regulation of soybean drought response by mepiquat chloride pretreatment

Comparative transcriptomic analyses of two sugarcane Saccharum L. cultivars differing in drought tolerance

Transcriptomics and metabolomics analysis reveal the dietary copper deficiency and supplementation effects of liver gene expression and metabolite change in grazing sheep

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