Effects of different extraction techniques on physicochemical properties and biological activities of polysaccharides from Fritillaria pallidiflora Schrenk

Abuduwaili, Aytursun; Rozi, Parhat; Mutailifu, Paiheerding; Gao, Yanhua; Nuerxiati, Rehebati; Aisa, Haji Akber; Yili, Abulimiti

doi:10.1016/j.procbio.2019.05.020

Cited by 57 publications

(17 citation statements)

References 47 publications

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“…[ 39 ] Other studies had shown that CHP‐2 retains an amorphous structure. [ 40 ] Overall, CHP‐2 was a composite system composed of crystalline, sub‐crystalline, and amorphous structures.…”

Section: Resultsmentioning

confidence: 99%

Structure of Cardamine hupingshanensis No. 2 Polysaccharide (CHP‐2) and Its Effect on Streptozotocin‐induced Diabetic Rats

Huang

Zhang

et al. 2021

Starch Stärke

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Four new types of water‐soluble polysaccharides are isolated from Cardamine hupingshanensis. Herein, this study examines C. hupingshanensis polysaccharide no. 2 (CHP‐2) with a molecular weight of 21.75 kDa. Analysis revealed that CHP‐2 is composed of d‐glucuronic acid, d‐galacturonic acid, glucose, galactose, and L‐arabinose. It is speculated that the main connecting bond is →4)‐α‐D‐Glc(p)‐(1→), →t)‐α‐D‐Glcp‐(1→). Through perfusion experiments using streptozotocin‐induced diabetic rats, oral CHP‐2 solution is found to significantly reduce food intake, water intake, and fasting blood glucose levels, while increasing the weights of rats. In addition, CHP‐2 significantly reduces the serum and liver malondialdehyde contents and increases the activity of the corresponding antioxidant enzymes. Moreover, the effect elicited by the CHP‐2 solution is similar to that of phenformin hydrochloride solution. Hence, the results of this study suggest that CHP‐2 exhibits blood sugar‐lowering and anti‐oxidation effects.

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Section: Resultsmentioning

confidence: 99%

Structure of Cardamine hupingshanensis No. 2 Polysaccharide (CHP‐2) and Its Effect on Streptozotocin‐induced Diabetic Rats

Huang

Zhang

et al. 2021

Starch Stärke

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“…Extraction processes influencing the bioactivity of extracts have been reported widely [ 37 , 38 ]. For fungal polysaccharides, a large number of factors, including their chemical components, molecular mass, structure, and conformation, can remarkably affect their antioxidant activities [ 8 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Optimization of ultrasonic-assisted extraction of polysaccharides and triterpenoids from the medicinal mushroom Ganoderma lucidum and evaluation of their in vitro antioxidant capacities

2020

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Ganoderma lucidum (Fr.) Krast, commonly known as “Lingzhi” in Chinese, is a medicinal mushroom that is rich in biologically active substances. Polysaccharides and triterpenoids are the two major components responsible for the bioactivity of this fungus. In the present study, the ultrasonic-assisted co-extraction (UACE) of polysaccharides and triterpenoids from G. lucidum was optimized using response surface methodology with a desirability function, with the equal importance for the two components. Following single factor experiments, the optimal conditions were determine as ultrasonic power of 210 W, extraction temperature of 80C, ratio of liquid to solid of 50 mL/g, and 100 min extraction time, using aqueous ethanol (50%, v/v) as the extracting solvent. Under the optimal conditions, the extraction yields of polysaccharides and triterpenoids reached 0.63% and 0.38%, respectively. On the basis of the scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl and evaluation of reducing power, the antioxidant capacities of the polysaccharides obtained by optimal UACE process were higher than those of polysaccharides extracted using traditional hot water extraction, whereas the triterpenoid-rich extracts showed antioxidant activities similar to those obtained using the ethanol maceration method. The present study is the first report on the simultaneous extraction of polysaccharides and triterpenoids from G. lucidum. The developed UACE process could be useful in preparation of a polysaccharide- and triterpenoid-rich ingredient that holds great promise for application in the Ganoderma industry.

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“…The samples in this stage all showed obvious weightlessness steps, which indicated that the samples were greatly decomposed by heat in this stage, which may be related to the thermal decomposition of polysaccharides (Wang, Zhang, et al, 2018). The third stage was 400-600℃; at this stage, the mass of the samples gradually decreases due to the thermal decomposition of carbon (Abuduwaili et al, 2019). Besides, RPs-3 maintained a high mass residue throughout the test.…”

Section: Thermogravimetric (Tg) Analysismentioning

confidence: 94%

Antioxidant activity and rheological properties of the polysaccharides isolated from Ribes stenocarpum maxim with different extraction methods

Qiao

Cai

et al. 2021

J. Food Process. Preserv.

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The purpose of the present study is to illustrate the effects of extraction methods on the antioxidant activity and rheological properties of polysaccharides isolated from Ribes stenocarpum Maxim (RPs). RPs were extracted by ultrasonic-assisted extraction (RPs-1), alkaline extraction (RPs-2), acid extraction (RPs-3), microwaveassisted extraction (RPs-4), and three-phase separation (RPs-5). The results showed that RPs-1, RPs-2, RPs-3, RPs-4, and RPs-5 are acidic heteropolysaccharides with glucose, arabinose, and galacturonic acid as the main chain units and absence of triple-helix structure. Of them, RPs-3 possess the highest uronic acid content, best thermal stability, and strongest DPPH, ABTS, and hydroxyl radical scavenging abilities. Rheological properties showed that the above-mentioned polysaccharides are non-Newtonian fluids and own the similar shear thinning properties. RPs-3 represent the highest apparent viscosity and better stability in the over-acid or overalkali environment. The above results indicated that the acid extraction could serve as the most favorable extraction method for RPs. Novelty impact statement: A novel polysaccharide was isolated from Ribes stenocarpumMaxim by acid extraction, showing a stronger antioxidant activity and excellent rheological properties, which has a good application prospect in food processing.

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Effects of different extraction techniques on physicochemical properties and biological activities of polysaccharides from Fritillaria pallidiflora Schrenk

Cited by 57 publications

References 47 publications

Structure of Cardamine hupingshanensis No. 2 Polysaccharide (CHP‐2) and Its Effect on Streptozotocin‐induced Diabetic Rats

Structure of Cardamine hupingshanensis No. 2 Polysaccharide (CHP‐2) and Its Effect on Streptozotocin‐induced Diabetic Rats

Optimization of ultrasonic-assisted extraction of polysaccharides and triterpenoids from the medicinal mushroom Ganoderma lucidum and evaluation of their in vitro antioxidant capacities

Antioxidant activity and rheological properties of the polysaccharides isolated from Ribes stenocarpum maxim with different extraction methods

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