Effects of dietary transgenic Bt corn pollen on larvae of<i>Apis mellifera</i>and<i>Galleria mellonella</i>

Hanley, Anne V.; Zhi, Huang; Pett, W.

doi:10.1080/00218839.2003.11101097

Cited by 60 publications

(49 citation statements)

References 19 publications

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“…Similarly, the overall mean weight of prepupae and also mean prepupal weight at the highest applied purified protein doses are in perfect range with the pollen feeding test (Hendriksma et al, 2011b). Our results on single Bt proteins further complement the less standardized colony level studies on single Cry1Ab or Cry1F maize pollen (Hanley et al, 2003) and the purified Cry3B protein (Arpaia, 1996), for which also no deleterious effect by Cry protein were found on honey bee Figure 2. Prepupal weight of protein exposed larvae (n=709).…”

Section: Toxicity Of Cry Proteins and Cp4supporting

confidence: 73%

Effects of multiple Bt proteins and GNA lectin on in vitro-reared honey bee larvae

Hendriksma¹,

Härtel²,

Babendreier³

et al. 2012

Apidologie

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-The honey bee is a key non-target arthropod in environmental risk assessments of genetically modified crops. We analyzed for the first time combined effects of three Bt proteins conferring insect resistances, and a CP4-protein conferring an herbicide resistance as simultaneously expressed in one GM maize. Furthermore, the biosafety of Galanthus nivalis agglutinin (GNA lectin), a candidate protein for pest control was tested. Under worst-case exposure scenario, by using controlled in vitro larvae rearing, the combination of Bt proteins showed no adverse effects on bee larvae. In contrast, the GNA lectin was toxic at a 144 h LD 50 of 16.3 μg/larva. The prepupal weight was found to differ between the larvae collection days and between the colonies used for the experiment, explaining up to five times more data variance than the protein treatments (N=709 prepupae). In conclusion, neither single nor a mix of different Bt proteins were found harmful to honey bee larvae.Apis mellifera / Bacillus thuringiensis / environmental risk assessment / genetically modified crops / Cry protein

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Section: Toxicity Of Cry Proteins and Cp4supporting

confidence: 73%

Effects of multiple Bt proteins and GNA lectin on in vitro-reared honey bee larvae

Hendriksma¹,

Härtel²,

Babendreier³

et al. 2012

Apidologie

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“…However, it must be considered that insecticide field applications differ from laboratory experiments. On the other hand, this data are in agreement with the majority laboratory studies published based on the effect of Btk produced by transgenic farming, which showed that the Btk does not affect honeybee populations (Hanley et al, 2003;Malone et al, 2004;Babendreier et al, 2005;O'Callaghan et al, 2005;Duan et al, 2008;Porcar et al, 2008). Furthermore, the results of the present study are consistent with previous works conducted in Bt corn pollen (Dai et al, 2012), which showed that the percentage of brood cells did not differ between the Bt and non-Bt treatments.…”

supporting

confidence: 83%

“…The effect on bees have been developed in some f ield and laboratory studies, although the majority of the experiments tested the toxicology of Btk used in genetically modif ied crops (O'Callaghan et al, 2005;Duan et al, 2008). These studies showed no adverse effect on A. mellifera (Malone & Pham-Delègue, 2001;Hanley et al, 2003;Malone et al, 2004;Porcar et al, 2008), however, some authors conf irmed a toxicity of Btk under controlled conditions (Ramirez-Romero et al, 2005;Brighenti et al, 2007). It is worth to note that it must be considered that the field results using Btk aerial application differ from the laboratory experiments and some authors require more information about effect under field apicultural conditions (Rose et al, 2007; …”

mentioning

confidence: 99%

Short communication. First field assessment of Bacillus thuringiensis subsp. kurstaki aerial application on the colony performance of Apis mellifera L. (Hymenoptera: Apidae)

Salord

Llado

Petro

et al. 2014

Span. j. agric. res.

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Honeybee populations around the world are experiencing a decrease in colony numbers probably due to a combination of different causes, such as diseases, poor nutrition and frequent applications of insecticides to control pests. Previous studies about the effect of pesticide Bacillus thuringiensis subsp. kurstaki (Btk) on Apis mellifera L. report different results. The aim of this study was to analyze the effect of field aerial applications of Btk on bee colony performance, specifically on the brood cell percentage evolution, which can be used as an indicator of queen health and brood development breeding rates. To achieve it, the brood cell surface was photographed in every sampling, and data were analyzed using a method based on image treatment software. A total of 480 pictures were examined from two groups of four nucleus hives in two areas, one receiving aerial spraying with Btk and the other without treatment. A mixed factorial design was realized to analyse the data showing no differences in colony performance between the two groups of colonies either before the treatment, during and at the end of the assay. Furthermore, the brood surface ratio of Btk-treated/untreated increased along the experiment. Therefore, the results of the present study suggest that Btk aerial applications did not affect the brood development of honeybees under natural conditions. Nevertheless further field studies are required to ascertain a safe use of Btk in forest pest management.

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“…proteinase inhibitors (PIs) (Brødsgaard et al, 2003;Burgess et al, 1996;Sagili et al, 2005), Bacillus thuringiensis (Bt) toxins (Arpaia, 1996;Malone et al, 2004) and biotinbinding proteins (Malone et al, 2002). Some have been conducted using transgenic plant material on adults (Babendreier et al, 2005;Liu et al, 2005), but few have considered the effects of transgenic plants on honey bee larval stages (Hanley et al, 2003). Larvae, undergoing growth, might be more sensitive to changes in food composition than adults.…”

Section: Introductionmentioning

confidence: 99%

Does pea lectin expressed transgenically in oilseed rape (Brassica napus) influence honey bee (Apis mellifera) larvae?

Lehrman

2007

Environ. Biosafety Res.

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The European honey bee (Apis mellifera) is important both for pollination and for honey production. Pollen is the major protein source for bees, which exposes them directly to changes in pollen quality e.g. through genetic engineering. In order to create a worst case scenario regarding pea lectin (PSL) expressed transgenically in oilseed rape anthers and pollen, the maximum amount of dried pollen that could be mixed in an artificial diet without negatively affecting larval performance (1.5% w/w) was fed to bee larvae. Pollen from two transgenic plant lines expressing PSL up to 1.2% of total soluble protein and pollen from one non-transgenic line was added to the same diet and used as a pollen control. When these three pollen diets and the control diet (without added pollen) were compared, no negative effect from the pollen of the transgenic plants could be detected on larval mortality, weight, or development time. An increased weight and a reduced developmental time were recorded for larvae on all diets containing pollen when compared to the diet without pollen.

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Effects of dietary transgenic Bt corn pollen on larvae ofApis melliferaandGalleria mellonella

Cited by 60 publications

References 19 publications

Effects of multiple Bt proteins and GNA lectin on in vitro-reared honey bee larvae

Effects of multiple Bt proteins and GNA lectin on in vitro-reared honey bee larvae

Short communication. First field assessment of Bacillus thuringiensis subsp. kurstaki aerial application on the colony performance of Apis mellifera L. (Hymenoptera: Apidae)

Does pea lectin expressed transgenically in oilseed rape (Brassica napus) influence honey bee (Apis mellifera) larvae?

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