Effects of Dietary Soybean Peptides on Hepatic Production of Ketone Bodies and Secretion of Triglyceride by Perfused Rat Liver

Tamaru, Shizuka; Kurayama, Takayuki; Sakono, Masafumi; Fukuda, Nobuhiro; Nakamori, Toshihiro; Furuta, Hitoshi; Tanaka, Kazunari; Sugano, Michihiro

doi:10.1271/bbb.70221

Cited by 19 publications

(12 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some of these functions have been reported to be due to soybean proteins and their digests. Tamaru et al [4] found that a low molecular weight peptide fraction from soybean protein isolate digested with Protin SD-AY10 displayed triglyceride-lowering activity in rats fed a cholesterol-enriched diet. Kim et al [5] reported that the peptide corresponding to residues 83 to 89 of soybean glycinin showed cytotoxic activity toward P388D1, a mouse monocyte macrophage cell line.…”

Section: Introductionmentioning

confidence: 99%

Soybean protein fraction digested with neutral protease preparation, “Peptidase R”, produced by Rhizopus oryzae, stimulates innate cellular immune system in mouse

Egusa

Otani

2009

International Immunopharmacology

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Soybean protein fraction digested with neutral protease preparation, “Peptidase R”, produced by Rhizopus oryzae, stimulates innate cellular immune system in mouse

Egusa

Otani

2009

International Immunopharmacology

View full text Add to dashboard Cite

show abstract

“…Our results showed that the low-molecular-weight (digested) peptide fraction of dietary soybean (LD) caused a reduction in triglyceride secretion from perfused rat liver (17). Post-perfused liver triglyceride levels were also lowered by LD feeding (17). These reductions were inversely related to the stimulation of fatty acid b-oxidation in the perfused rat liver (17).…”

mentioning

confidence: 76%

“…Post-perfused liver triglyceride levels were also lowered by LD feeding (17). These reductions were inversely related to the stimulation of fatty acid b-oxidation in the perfused rat liver (17). Furthermore, the responses induced by LD appeared to be greater than those induced by intact SPI (17).…”

mentioning

confidence: 88%

See 1 more Smart Citation

Dietary Soybean Peptides Containing a Low-Molecular Fraction Can Lower Serum and Liver Triglyceride Levels in Rats

Tamaru

Kurayama

Sakono

et al. 2014

J Nutr Sci Vitaminol

Self Cite

View full text Add to dashboard Cite

The cholesterol-lowering effect of dietary soybean protein has been experimentally established in both animal and human subjects (1-3). The various mechanisms underlying the hypocholesterolemic action of soybean protein include the suppression of intestinal cholesterol absorption, promotion of fecal steroid excretion, and increases in the rate of low-density lipoprotein (LDL) receptor-mediated cholesterol removal (3-11). Soybean peptides containing high-molecular-weight (undigested) peptide fractions, obtained through the digestion of soybean protein isolate (SPI), are believed to play an important role in the hypocholesterolemic effect of soybean proteins (6-9). High-molecular-weight soybean peptides are responsible for bile acid-binding, which results in the stimulation of fecal steroid excretion (6-9).Soybean protein also has a hypotriglyceridemic effect that is mediated by the stimulation of fatty acid b-oxidation, suppression of fatty acid and triglyceride synthesis, and suppression of hepatic triglyceride secretion (3,(12)(13)(14)(15)(16). We have previously investigated the effect of certain soybean peptides, obtained through the digestion of SPI, on triglyceride metabolism in rats (17). Our results showed that the low-molecular-weight (digested) peptide fraction of dietary soybean (LD) caused a reduction in triglyceride secretion from perfused rat liver (17). Post-perfused liver triglyceride levels were also lowered by LD feeding (17). These reductions were inversely related to the stimulation of fatty acid b-oxidation in the perfused rat liver (17). Furthermore, the responses induced by LD appeared to be greater than those induced by intact SPI (17). Therefore, LD may modulate triglyceride metabolism and contains the component(s) responsible for the hypotriglyceridemic activity of soybean protein. Soybean pentapeptides (18) and oligopeptides (19) have been recently implicated as the components responsible for the triglyceride-lowering effect of soybeans. However, the particular peptides in soybean protein responsible for the triglyceride-lowering effect have not been identified. In this study, we investigated the effect of dietary LD on lipid parameters, particularly serum and liver triglyceride levels, in rats. MATERIALS AND METHODSPreparation of soybean peptides. Soybean protein isolate (SPI, Fujipro R, Fuji, Osaka, Japan) was added to 10 volumes of water, followed by artificial digestion with a commercial protease (protein AY10; Amano Enzyme, Summary We investigated the effects of dietary soybean peptides, particularly lowmolecular-weight peptides, on serum and hepatic concentrations of lipids in rats. Soybean protein isolate (SPI) was digested with protease to produce low-molecular-weight peptides (LD) or a mixture of high-and low-molecular-weight peptides (HLD). Rats were fed diets containing 20% casein, SPI, LD or HLD as a nitrogen source, with or without 0.5% cholesterol, for 2 wk. Next, rats were fed cholesterol-free diets containing 0%, 5%, 10%, or 20% LD at the expense of casein for 2 wk. Seru...

show abstract

“…The average molecular weight of SP is about 3-10 kDa, and it is characterized to have a high glutamic acid content. Reported actions of SP include a cholesterol-inhibitory effect, 13) a weight loss effect, 14) an anti-fatigue effect, 15) and an antihypertensive effect. 16) Low molecular weight SP (LSP), which is a SP that has been further broken down, has an average molecular weight of about 500-1,000 Da; like SP, it has a high glutamic acid content.…”

mentioning

confidence: 99%

Effects of low molecular weight soybean peptide on mRNA and protein expression levels of differentiation markers in normal human epidermal keratinocytes

Tokudome

Nakamura

Hashimoto

2014

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Low molecular weight soybean peptide (LSP) was applied to normal human epidermal keratinocytes, and the results showed a significant increase in the gene expression levels of involucrin, transglutaminase, and profilaggrin. Filaggrin protein levels were also significantly higher. It is possible that LSP has an epidermal cell differentiation-promoting effect and may be able to regulate metabolism of the epidermis.

show abstract

Effects of Dietary Soybean Peptides on Hepatic Production of Ketone Bodies and Secretion of Triglyceride by Perfused Rat Liver

Cited by 19 publications

References 31 publications

Soybean protein fraction digested with neutral protease preparation, “Peptidase R”, produced by Rhizopus oryzae, stimulates innate cellular immune system in mouse

Soybean protein fraction digested with neutral protease preparation, “Peptidase R”, produced by Rhizopus oryzae, stimulates innate cellular immune system in mouse

Dietary Soybean Peptides Containing a Low-Molecular Fraction Can Lower Serum and Liver Triglyceride Levels in Rats

Effects of low molecular weight soybean peptide on mRNA and protein expression levels of differentiation markers in normal human epidermal keratinocytes

Contact Info

Product

Resources

About