Effects of dietary cysteamine supplementation on growth performance and whole-body protein turnover in finishing pigs

Liu, Guangmang; Wang, Zhisheng; Wu, De; An-guo, Zhou; Liu, Guilian

doi:10.1016/j.livsci.2008.07.027

Cited by 16 publications

(18 citation statements)

References 19 publications

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“…Our results were partially consistent with the findings of Yang et al (), who reported that the addition of cysteamine hydrochloride (CSH) at 60 and 90 mg /kg increased the activities of protease, lipase and amylase in the pancreas and small intestinal contents of broilers. However, Liu et al () reported that CS supplementation did not affect nitrogen digestibility, indicating that CS could increase growth performance possibly through modifying protein synthesis and protein degradation but not protein digestion in pigs. The different results may be due to the type, purity, concentration and particles of CS used in the study.…”

Section: Discussionmentioning

confidence: 99%

Effects of cysteamine supplementation on the intestinal expression of amino acid and peptide transporters and intestinal health in finishing pigs

Zhou

Luo

Zhang

et al. 2016

Animal Science Journal

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This study aimed to evaluate the effects of cysteamine supplementation on the expression of jejunal amino acid and peptide transporters and intestinal health in finishing pigs. Sixty barrows were allocated into two experimental diets consisting of a basal control diet supplemented with 0 or 142 mg/kg cysteamine. After 41 days, 10 pigs per treatment were slaughtered. The results showed that cysteamine supplementation increased the apparent digestibility of crude protein (CP) (P < 0.05) and the trypsin activity in jejunal digesta (P < 0.01). Cysteamine supplementation also increased the messenger RNA abundance of SLC7A7, SLC7A9 and SLC15A1, occludin, claudin-1 and zonula occludens protein-1 (P < 0.001) in the jejunum mucosa. Increased glutathione content (P < 0.01) and glutathione peroxidase activity (P < 0.05) and decreased malondialdehyde content (P < 0.01) were observed in pigs receiving cysteamine. Additionally, cysteamine supplementation increased the concentrations of secretory immunoglobulin A (IgA) (P < 0.05), IgM (P < 0.001) and IgG (P < 0.001) in the jejunal mucosa. It is concluded that cysteamine supplementation could influence protein digestion and absorption via increasing trypsin activity, enhancing the digestibility of CP, and promoting the expression of jejunal amino acid and peptide transporters. Moreover, cysteamine improved intestinal integrity, antioxidant capacity and immune function in the jejunum, which were beneficial for intestinal health.

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Section: Discussionmentioning

confidence: 99%

Effects of cysteamine supplementation on the intestinal expression of amino acid and peptide transporters and intestinal health in finishing pigs

Zhou

Luo

Zhang

et al. 2016

Animal Science Journal

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“…Cysteamine (CS; mercaptoethylamine, HS-CH 2 -CH 2 -NH 2 ) is used as a feed additive in animal nutrition to improve the growth performance and feed efficiency of broilers, carp and pigs [ 1 – 3 ]. CS also reduces back fat thickness and improves the lean percentage of pigs [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…CS also reduces back fat thickness and improves the lean percentage of pigs [ 4 ]. Previous studies have demonstrated that CS decreases somatostatin (SS) levels in tissue and the hypothalamus, which leads to an increase in growth hormone (GH) secretion [ 3 ]. GH improves growth performance by increasing the production of insulin-like growth factor 1 (IGF-1), consequently stimulating protein synthesis and reducing the rate of protein degradation in muscle [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…in vivo . Additionally, the results of a protein turnover trial on finishing pigs showed that CS supplementation increased protein deposition due to a decrease in protein degradation, but it did not change the rate of protein synthesis [ 3 ]. However, the mechanisms responsible for the changes in anabolic and catabolic metabolism have not been delineated.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Dietary Crude Protein Levels and Cysteamine Supplementation on Protein Synthetic and Degradative Signaling in Skeletal Muscle of Finishing Pigs

Zhou

Zhang

et al. 2015

PLoS ONE

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Dietary protein levels and cysteamine (CS) supplementation can affect growth performance and protein metabolism of pigs. However, the influence of dietary protein intake on the growth response of CS-treated pigs is unclear, and the mechanisms involved in protein metabolism remain unknown. Hence, we investigated the interactions between dietary protein levels and CS supplementation and the effects of dietary crude protein levels and CS supplementation on protein synthetic and degradative signaling in skeletal muscle of finishing pigs. One hundred twenty barrows (65.84 ± 0.61 kg) were allocated to a 2 × 2 factorial arrangement with five replicates of six pigs each. The primary variations were dietary crude protein (CP) levels (14% or 10%) and CS supplemental levels (0 or 700 mg/kg). The low-protein (LP) diets (10% CP) were supplemented with enough essential amino acids (EAA) to meet the NRC AA requirements of pigs and maintain the balanced supply of eight EAA including lysine, methionine, threonine, tryptophan, valine, phenylalanine, isoleucine, and leucine. After 41 days, 10 pigs per treatment were slaughtered. We found that LP diets supplemented with EAA resulted in decreased concentrations of plasma somatostatin (SS) (P<0.01) and plasma urea nitrogen (PUN) (P<0.001), while dietary protein levels did not affect other traits. However, CS supplementation increased the average daily gain (P<0.001) and lean percentage (P<0.05), and decreased the feed conversion ratio (P<0.05) and back fat (P<0.05). CS supplementation also increased the concentrations of plasma insulin-like growth factor 1 (IGF-1) (P<0.001), and reduced the concentrations of leptin, SS, and PUN (P<0.001). Increased mRNA abundance of Akt1 and IGF-1 signaling (P<0.001) and decreased mRNA abundance of Forkhead Box O (FOXO) 4 (P<0.01) and muscle atrophy F-box (P<0.001) were observed in pigs receiving CS. Additionally, CS supplementation increased the protein levels for the phosphorylated mammalian target of rapamycin (mTOR), eIF-4E binding protein 1, and ribosomal protein S6 kinase 1 (P<0.001). There were no interactions between dietary protein levels and CS supplementation for all traits. In conclusion, dietary protein levels and CS supplementation influenced growth and protein metabolism through independent mechanisms in pigs. In addition, LP diets supplemented with EAA did not affect growth performance and other traits except the concentrations of SS and PUN probably through maintenance of protein synthesis and degradation signaling. Moreover, CS supplementation improved growth performance by increasing plasma IGF-1 concentrations possibly through alterations of mTOR and Akt/FOXO signaling pathways in skeletal muscle of finishing pigs.

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“…Long-term CS treatment can modulate insulinlike growth factor systems in animals (Liu et al 2008). Cysteamine supplementation can increase protein deposition, mainly through a decrease in protein breakdown (Liu et al 2009). Gavage feeding of very high doses of CS to rats reliably generates duodenal ulcers within 24 h (Khomenko et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Metabolomic studies on the biochemical profile of urine from rats with acute cysteamine supplementation

Liu

Wang

et al. 2011

Metabolomics

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This study investigates the effect of acute cysteamine (CS) supplementation on rat metabolism. A metabolomic strategy using high-resolution 1 H-NMR spectroscopy in conjunction with principal component analysis was applied to examine rat biological responses to CS administration. Half of female Sprague-Dawley rats (2 groups of 6 rats) were each given doses of 150 mg CS/kg body weight intraperitoneally. Urine samples were collected twice daily (0-8 and 8-24 h) from the rats following CS administration. The identifiable biochemical effects associated with CS supplementation included decreased urinary concentrations of hippurate, succinate, citric acid, and 2-oxoglutarate, as well as increased urinary concentrations of dimethylamine, dimethylglycine, glycine, and taurine. These effects were predominately seen within the first 8 h after CS administration. Clear differences in succinate, citric acid, and 2-oxoglutarate were observed 8-24 h following CS. The results suggest that CS supplementation in the rats resulted in modulation of intestinal microbial metabolism and metabolic perturbation of the tricarboxylic acid cycle.

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Effects of dietary cysteamine supplementation on growth performance and whole-body protein turnover in finishing pigs

Cited by 16 publications

References 19 publications

Effects of cysteamine supplementation on the intestinal expression of amino acid and peptide transporters and intestinal health in finishing pigs

Effects of cysteamine supplementation on the intestinal expression of amino acid and peptide transporters and intestinal health in finishing pigs

Effects of Dietary Crude Protein Levels and Cysteamine Supplementation on Protein Synthetic and Degradative Signaling in Skeletal Muscle of Finishing Pigs

Metabolomic studies on the biochemical profile of urine from rats with acute cysteamine supplementation

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