Effects of cysteamine supplementation on the intestinal expression of amino acid and peptide transporters and intestinal health in finishing pigs

Zhou, Ping; Luo, Yiqiu; Zhang, Lin; Li, Jiaolong; Zhang, Bolin; Shen, Xing; Zhu, Yanhua; Gao, Feng; Zhou, Guanghong

doi:10.1111/asj.12626

Cited by 19 publications

(25 citation statements)

References 43 publications

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“…In the present study, C. perfringens challenge downregulated CLDN1 and ZO-2, indicating the disruption of tight junction barrier, and this was attenuated by the supplementation of CSH. Similar effects were found in studies that cysteamine supplementation increased the mRNA abundance of tight junction, including occludin, claudin-1, and zonula occludens protein-1, in the jejunum mucosa of finishing pigs (Zhou et al, 2017) and N-acetyl-L-cysteine protected occludin, zona occludens-1, claudin-3, E-cadherin, and beta-catenin, as well as the actin cytoskeleton in mouse colon (Shukla et al, 2016).…”

Section: Discussionsupporting

confidence: 84%

“…Indeed, in the present study, the serum TNF-α, IL-1β, and IL-6 were increased by C. perfringens challenge, and were decreased by CSH. In farm animals, cysteamine supplementation increased the concentrations of secretory IgA, IgM, and IgG in the jejunal mucosa of pigs (Zhou et al, 2017), and induced proliferation and differentiation of IgA-positive cells and intraepithelial lymphocytes in the intestinal mucosa of chickens by reducing the number of somatostatin-positive cells (Yang et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Wilmer et al (2011) argued that cysteamine did not affect protein turnover, total glutathione levels, protein oxidation, and lipid peroxidation, but normalized glutathione redox status in cystinotic cells. Zhou et al (2017) reported that cysteamine increased glutathione and its peroxidase, and decreased MDA in pigs. Also, the CSH improved antioxidant status and delayed pig meat discoloration by improving glutathione levels and anti-oxidase activity (Bai et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…As a feed additive, cysteamine hydrochloride (CSH) is the simplest and the most stable among aminothiol compounds (Reid, 1958). It is well documented that thiol-containing compounds have a biological role in anti-oxidation and anti-inflammation (Morrison et al, 2005;Zhou et al, 2017;Bai et al, 2018). Recent studies have demonstrated their antimicrobial and antibiofilm activity in cystic fibrosis models (Charrier et al, 2014;Fraser-Pitt et al, 2016.…”

Section: Introductionmentioning

confidence: 99%

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Dietary cysteamine hydrochloride protects against oxidation, inflammation, and mucosal barrier disruption of broiler chickens challenged with Clostridium perfringens

Liu

Lin

Wang

et al. 2018

Journal of Animal Science

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This study aimed to investigate the effect of dietary cysteamine hydrochloride (CSH) on the growth performance, oxidation, inflammation, and gene expression of cytoskeleton and tight junction in the intestinal mucosa of broiler chickens challenged with Clostridium perfringens (C. perfringens). A total of 360 one-day-old broiler chicks were randomly distributed into 5 groups for a negative control (NC, without C. perfringens challenge), a positive control (PC, with C. perfringens challenge), and PC plus CSH at 100, 150, or 200 mg/kg of diet. The results showed that average daily gain, gain:feed, cecal population and enterotoxin of C. perfringens were negatively affected (P < 0.05) by the C. perfringens challenge, but were conversely affected (P < 0.05) by the CSH supplementation, and G:F reached to the level of NC group. The PC group increased (P < 0.05) serum diamine oxidase, malondialdehyde, protein carbonyl, interleukin-6, interleukin-1β, and tumor necrosis factor-α, whereas the supplementation of CSH decreased (P < 0.05) these parameters. Moreover, the C. perfringens challenge worsened the disruption of intestinal mucosal cytoskeleton and tight junction by downregulating (P < 0.05) the mRNA levels of actin protein of muscle Z-line alpha, syncoilin, synemin, tubulin, claudin-1, and zona occludens protein-2, while these parameters were partially compensated (P < 0.05) by CSH supplementation. For the dose trends of CSH, there were linear and quadratic (P < 0.05) effects on gain:feed, enterotoxins, tumor necrosis factor-α, tubulin alpha 1c, syncoilin, and synemin. In conclusion, the CSH can be an alternative against C. perfringens infection by beneficially regulating gut pathogenic bacteria and enterotoxins, oxidation, inflammation, cytoskeleton, and tight junction in broiler chickens.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dietary cysteamine hydrochloride protects against oxidation, inflammation, and mucosal barrier disruption of broiler chickens challenged with Clostridium perfringens

Liu

Lin

Wang

et al. 2018

Journal of Animal Science

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“…Besides, studies in broilers indicated that l ‐theanine can affect intestinal morphology, microflora and physical barrier‐related gene expression, but less information is available concerning the role of l ‐theanine as a feed additive in broiler production. The antioxidant system plays an important role in maintaining intestinal barrier integrity . Our previous study found that the beneficial effect of l ‐theanine on intestinal morphology of duck can be partly attributed to the increased antioxidant capacity of intestinal mucosa .…”

Section: Introductionmentioning

confidence: 96%

Effect of l‐theanine on growth performance, intestinal development and health, and peptide and amino acid transporters expression of broilers

et al. 2020

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BACKGROUND: L-Theanine has multiple beneficial biological activities. However, there is little information about the use of L-theanine in broiler production. Therefore, this study investigated the effect of L-theanine on growth performance, intestinal development and health, and the mRNA levels of intestinal peptide and amino acid (AA) transporters of broilers. RESULTS: Body weight and average daily gain were increased by L-theanine, whereas feed to gain ratio was decreased (quadratic, P < 0.05). Notably, the relative weight of duodenum, jejunum and ileum, villus height, villus height to crypt depth ratio, the jejunal activities of glutathione peroxidase, total antioxidant capacity, catalase and total superoxide dismutase were increased linearly and/or quadratically by L-theanine (P < 0.05), whereas crypt depth, serum D-lactic acid, and jejunal protein carbonyls and malondialdehyde content were decreased linearly and/or quadratically (P < 0.05). Moreover, L-theanine enhanced the jejunal mRNA levels of occludin, claudin-1, E-cadherin, zona occludens-1, di-and tripeptide transporter, excitatory AA transporter 3, Na + -independent cationic AA transporter 1, Na + -independent cationic and zwitterionic AA transporter, Na + -and Cl − -dependent neutral and cationic AA transporter, Na + -independent cationic and Na + -dependent neutral AA transporter (y+LAT) 1, y+LAT2, Na + -independent branched-chain and aromatic AA transporter, and heavy chain corresponding to the b ∘,+ transport system (linear and/or quadratic, P < 0.05). CONCLUSIONS: L-Theanine beneficially affected the growth performance of broilers by improving intestinal development andhealth, and the intestinal mRNA levels of AA and peptide transporters. Therefore, L-theanine has the potential to be a promising feed additive for broilers. Jejunum morphology analysisJejunum morphology was measured as previously described. 21,22 Briefly, the formalin-fixed jejunal samples were embedded in paraffin wax, cut into about 5 μm transverse sections, mounted on glass slides, and then stained with hematoxylin and eosin. Crypt depth and villus height were determined with an image processing and analysis system (Leica Imaging Systems Ltd, Cambridge, UK). Fifteen well-orientated villi and their adjacent crypts from each intestinal sample were measured. Serum D-lactic acid content and jejunum antioxidant status analysisThe mucosa of jejunum was homogenized in ice-cold physiological saline solution (1:9, w/v). The homogenate was centrifuged at 2500 × g for 8 min at 4 ∘ C and then the supernatant was collected for antioxidant enzyme activity and metabolite content analysis at appropriate dilution. The protein concentration of the supernatant was assayed by the Bradford method with bovine serum albumin as the standard. The content of serum D-lactic acid and RESULTS Growth performanceGrowth performance data are shown in Table 3. L-Theanine elevated BW at days 21 and 42 (quadratic, P < 0.05). From days 1 to 21, L-theanine increased ADG (quadratic, P < 0.05) and decreased

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Cysteamine administration in lambs grazing on mountain pastures: Effects on the body weight, antioxidant capacity, thyroid hormones and growth hormone secretion

Shokrollahi

Fazli

Morammazi

et al. 2021

Veterinary Medicine & Sci

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This study aimed to evaluate the effects of intravenous injection of cysteamine (CS) on body weight (BW), growth hormone (GH), thyroid hormones (TH) secretion, and antioxidant status of growing lambs grazing on mountain pastures. Fifteen lambs (3-4 months of age) were randomly allocated into three experimental groups which received different dosages of CS: 0, 20, and 50 mg/kg BW −1 . The CS was injected on the 1st, 10th , and 20th days of the experiment to the lambs through the jugular vein.Assessment of plasma concentration of GH and TH hormones was carried out at days 0 (a day before the start of CS injections), 15, and 30 of the experiment. The antioxidant enzymes were measured at the end of the experiment. Lambs were weighed at days 0, 10, 20, and 30 of the experiment. The results showed that treatment and time affected the BW, GH, triiodothyronine (T 3 ), and tetraiodothyronine (T 4 ) secretion. The intravenous injection of CS increased the BW of growing lambs (p < 0.01) and increased the plasma concentration of GH, T3, and T4 (p < 0.01). The treatment also enhanced glutathione peroxidase (GSH-Px; p < 0.05) and reduced malondialdehyde concentrations (MDA; p < 0.01). Total antioxidant capacity (T-AOC) level reduced in CS-1 treatment compared to GC and CS-2 treatments (p < 0.01). The levels of superoxide dismutase (SOD) and catalase (CAT) were not affected by CS. In conclusion, intravenous injection of CS improved BW, GH, and TH concentrations and antioxidant capacity in growing lambs grazing on mountain pastures.

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Effects of cysteamine supplementation on the intestinal expression of amino acid and peptide transporters and intestinal health in finishing pigs

Cited by 19 publications

References 43 publications

Dietary cysteamine hydrochloride protects against oxidation, inflammation, and mucosal barrier disruption of broiler chickens challenged with Clostridium perfringens

Dietary cysteamine hydrochloride protects against oxidation, inflammation, and mucosal barrier disruption of broiler chickens challenged with Clostridium perfringens

Effect of l‐theanine on growth performance, intestinal development and health, and peptide and amino acid transporters expression of broilers

Cysteamine administration in lambs grazing on mountain pastures: Effects on the body weight, antioxidant capacity, thyroid hormones and growth hormone secretion

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