Metabolomic studies on the biochemical profile of urine from rats with acute cysteamine supplementation

Abstract

This study investigates the effect of acute cysteamine (CS) supplementation on rat metabolism. A metabolomic strategy using high-resolution 1 H-NMR spectroscopy in conjunction with principal component analysis was applied to examine rat biological responses to CS administration. Half of female Sprague-Dawley rats (2 groups of 6 rats) were each given doses of 150 mg CS/kg body weight intraperitoneally. Urine samples were collected twice daily (0-8 and 8-24 h) from the rats following CS administration. The identifiable biochemical effects associated with CS supplementation included decreased urinary concentrations of hippurate, succinate, citric acid, and 2-oxoglutarate, as well as increased urinary concentrations of dimethylamine, dimethylglycine, glycine, and taurine. These effects were predominately seen within the first 8 h after CS administration. Clear differences in succinate, citric acid, and 2-oxoglutarate were observed 8-24 h following CS. The results suggest that CS supplementation in the rats resulted in modulation of intestinal microbial metabolism and metabolic perturbation of the tricarboxylic acid cycle.

Comparison of GC-MS and NMR for Metabolite Profiling of Rice Subjected to Submergence Stress

“…Similar to what was previously reported by 1 H NMR, 24 the GC-MS analysis presented in Figure 3 shows that 15 of the 17 detected amino acids also accumulated during submergence and showed a return toward pretreatment values after 24 h of recovery. Significant differences (P value ≤ 0.05) between the genotypes were evident in the levels of 11 of the 17 detected amino acids either at some time point of the submergence treatment or after 24 h of recovery (Table 1).…”

“…There were four biological replicates for each treatment, with each biological replicate consisting of a homogeneous tissue pool from the plants in one pot (n = 25). Aliquots of each tissue pool were also analyzed using 1 H NMR as reported previously. 24…”

“…Metabolomics is a rapidly growing field of research providing information downstream of genetic responses to biotic and abiotic stressors. Many metabolomics studies have been conducted with mammals to better understand human diseases and in plant systems with the intent of improving disease resistance and crop production; however, results have also been reported for fungi, bacteria, fish, and other organisms. − Because of the complexity of the samples interrogated, the analytical methods for metabolomics studies need to have both high resolving power and the ability to provide robustly quantitative results. Several analytical techniques have been used for metabolomics and metabolite profiling including capillary electrophoresis–mass spectrometry (CE-MS), liquid chromatography–mass spectrometry (LC-MS), gas chromatography–mass spectrometry (GC-MS), , and nuclear magnetic resonance (NMR). , …”

Prolonged antibiotic use induces intestinal injury in mice that is repaired after removing antibiotic pressure: implications for empiric antibiotic therapy

“…Hippurate is produced from benzoate (Beliveau and Brusilow 1987); however, altered gut metabolism could lead to reduced benzoate production and ultimately hippurate synthesis (Williams et al 2010; Liu et al 2011). Allantoin decreased in A mice urine due to reduced protein break-down into purine and pyrimidine bases by gut microflora, reduced absorption through the intestinal wall, and reduced allantoin end product excreted in urine (Chen et al1992; Condon and Hatfield 1970).…”

1H-NMR metabonomics study of the therapeutic mechanism of total alkaloids and ajmalicine from Rauvolfia verticillata in spontaneously hypertensive rats

“…Rather than examining just one or two specic metabolites, the study is a multi-targeted analysis of low molecular weight, endogenous metabolites. 18 Although there have been many metabonomic studies on the actions of traditional Chinese medicines, [19][20][21][22][23] no such studies on the total alkaloids and ajmalicine have been reported to date.…”