Effects of dietary beta-carotene on the immune response of rainbow trout Oncorhynchus mykiss

Amar, Edgar C.; Kiron, Viswanath; Satoh, Shuichi; Okamoto, Nobuaki; Watanabe, Takeshi

doi:10.1046/j.1444-2906.2000.00170.x

Cited by 147 publications

(112 citation statements)

References 29 publications

(66 reference statements)

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“…The protein in the blood plasma was measured using the Total Protein kit according to the manufacturer's instructions (Lab Test ® , Lagoa Santa, MG, Brazil). The total immunoglobulin concentration was measured after infection (AMAR et al, 2000).…”

Section: Methodsmentioning

confidence: 99%

Probiotic supplementation in diet and vaccination of hybrid surubim (Pseudoplatystoma reticulatum♀ x P. corruscans♂)

et al. 2016

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Section: Methodsmentioning

confidence: 99%

Probiotic supplementation in diet and vaccination of hybrid surubim (Pseudoplatystoma reticulatum♀ x P. corruscans♂)

et al. 2016

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“…Alternative complement activity. Alternative complement haemolytic activity (ACH 50 ) was assayed following the procedure of Yano (1992), based on the haemolysis of rabbit red blood cells (RaRBC) as described in Amar et al (2000). The volume yielding 50% haemolysis was used for determining the complement activity of the plasma sample as follows:…”

mentioning

confidence: 99%

Effect of chemotherapy, vaccines and immunostimulants on innate immunity of goldfish infected with Aeromonas hydrophila

Harikrishnan¹,

Balasundaram²,

Heo³

2009

Dis. Aquat. Org.

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We report the effect of various chemicals, vaccines and immunostimulants on innate immune mechanisms such as phagocytosis activity, superoxide anion production of blood leukocytes, complement activity and plasma lysozyme activity and disease resistance in goldfish Carassius auratus (23 ± 2 g) against Aeromonas hydrophila on Days 15 and 30 post-infection. In infected fish, the administration of diets supplemented with either probiotics, tri-herbal extract or azadirachtin for 30 d significantly enhanced phagocytic activity; administration for 15 d had no effect. In fish treated with heat-killed and formalin-killed vaccines, and probiotics-, tri-herbal-and azadirachtin-supplemented diets, superoxide anion production was enhanced on Day 30. However, there was no superoxide anion production in fish treated with tetracycline, furanace, formalin or hydrogen peroxide. Complement activity was also significantly enhanced on Days 15 and 30 in fish groups treated with vaccine and probiotics-, tri-herbal-and azadirachtin-supplemented diets. Other groups had no complement activity. Lysozyme activity was significantly enhanced after 30 d in fish treated with heat-killed vaccine and probiotics-, tri-herbal-and azadirachtin-supplemented diets. The probiotics-and tri-herbalcontaining diet administration groups had no mortality (0%) preceding the challenge with live A. hydrophila. Groups treated with heat-or formalin-killed vaccines or azadirachtin-supplemented diet suffered mortalities of 30, 35 and 10%, respectively, which represents an improvement of survival rate compared to untreated infected control groups. We conclude that probiotics-, tri-herbaland azadirachtin-supplemented diets act as immunostimulants enhancing the goldfish innate immune response and disease resistance against A. hydrophila. KEY WORDS: Nonspecific immune parameter · Carassius auratus · Aeromonas hydrophila · Tri-herbal · Probiotic · Azadirachtin Resale or republication not permitted without written consent of the publisherDis Aquat Org 88: [45][46][47][48][49][50][51][52][53][54] 2009 (Areerat 1987) and other regions of the world (Amin et al. 1985). A. hydrophila routinely isolated from healthy and moribund fish produce cytotoxins, proteases, surface (S)-layers and aerolysin toxins (Cahill 1990, Paniagua et al. 1990, Rahman et al. 1997.Aeromonas hydrophila infection can be controlled with antibiotics and chemicals. Traditional use of oral antibiotics such as oxytetracycline, chloramphenicol and nifurpirinol remains the most convenient form of therapy for motile aeromonad septicaemia (Noga 1996). However, antibiotic treatment is costprohibitive to farmers in many undeveloped and developing countries. Extensive use of antibiotics may result in residues (Shao 2001) and an increase in multidrug-resistant bacteria in human and veterinary medicine (Bryan 1984). Therefore, vaccination is a better choice to control A. hydrophila infections. At present, no vaccines for the protection of farmed fish against A. hydrophila infections are commercially av...

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“…Several authors (Amar, Kiron, Satoh, & Okamoto, 2000;Amar, Kiron, Satoh & Watanabe, 2001;Amar, Kiron, Satoh & Watanabe, 2004) revealed effects of feeding carotenoids from both natural and synthetic sources on the non-specific immune responses of rainbow trout and found augmented non-specific responses along with protection from pathogenic challenge in vivo. The resistance of rainbow trout to infectious hematopoietic necrosis virus (IHNV) showed significant improvement after oral ingestion of natural and synthetic carotenoids, particularly astaxanthin (Amar, Kiron, Akutsu, Satoh & Watanabe, 2012).…”

Section: Turkish Journal Of Fisheries and Aquatic Sciencesmentioning

confidence: 99%

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Gopan¹,

Ande²,

Varghese³

et al. 2018

Turk. J. Fish. Aquat. Sci.

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A feeding trial of 45 days was conducted for delineating the effect of carotenoids on the fillet appearance, antioxidant status and nonspecific immune responses of Pangasianodon hypophthalmus fingerlings. Two hundred and fifty two fingerlings were randomly distributed into seven different experimental groups in triplicates. Seven isonitrogenous and isolipidic diets were prepared with two levels (150 and 300and a control diet (T1) without any carotenoids. The experimental fish were fed daily at the rate of 3% body weight in two instalments. At the end of the experiment, the fish were sampled to evaluate the colour and texture parameters, of fillets along with antioxidant and nonspecific immune status of fish. The fillet colour exhibited significantly higher (p<0.05) intensity of redness in the groups fed astaxanthin and a higher yellowish colouration in the beta-carotene fed groups. Significant (p<0.05) reduction in the activities of antioxidant enzymes and a significantly (p<0.05) higher PUFA/SFA ratio was also observed in astaxanthin fed groups. The total erythrocyte count, haemoglobin, respiratory burst, lysozyme activities were also enhanced in both astaxanthin fed groups (T2 & T3). The effect of carotenoids in modulating the texture of pangasius fillets was diminutive and further, there was little influence of carotenoids on growth. Thus, from this study, it can be concluded that astaxanthin supplementation can improve fillet appearance, antioxidant status and enhance the nonspecific immune response.

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Effects of dietary beta-carotene on the immune response of rainbow trout Oncorhynchus mykiss

Cited by 147 publications

References 29 publications

Probiotic supplementation in diet and vaccination of hybrid surubim (Pseudoplatystoma reticulatum♀ x P. corruscans♂)

Probiotic supplementation in diet and vaccination of hybrid surubim (Pseudoplatystoma reticulatum♀ x P. corruscans♂)

Effect of chemotherapy, vaccines and immunostimulants on innate immunity of goldfish infected with Aeromonas hydrophila

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