Effects of dietary amino acids in ameliorating intestinal function during enteric challenges in broiler chickens

Bortoluzzi, Cristiano; Fernandes, Jovanir Inês Müller; Doranalli, Kiran; Applegate, T. J.

doi:10.1016/j.anifeedsci.2019.114383

Cited by 18 publications

(8 citation statements)

References 112 publications

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“…The lack of effect of supplemental Gln on the studied amino acid transporters, further highlights that under conditions of the present study, Gln had not been limiting even though dietary protein had been reduced in the basal diets to 194 g/kg. It should be noted that the homeostasis of Gln is maintained through different and complex mode of transport mediated by membrane transporters (Bortoluzzi, Fernandes, Doranalli, & Applegate, 2020).…”

Section: Discussionmentioning

confidence: 99%

Expression of selected genes encoding mechanistic pathways, nutrient and amino acid transporters in jejunum and ileum of broiler chickens fed a reduced protein diet supplemented with arginine, glutamine and glycine under stress stimulated by dexamethasone

Barekatain

Chrystal

Gilani

et al. 2020

Animal Physiology Nutrition

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Reducing crude protein and supplementation with synthetic amino acids in poultry nutrition is a recent trend to avoid wastage of protein and ammonia in production systems. Stress has been shown to impair intestinal barrier and increase inflammatory response. This study was performed on intestinal tissues of broiler chickens to understand the mechanism of stress induced by a synthetic glucocorticoid, dexamethasone (DEX) and the effect of supplementation of arginine, glutamine and glycine in reduced protein diets. Intestinal tissue samples from a previous study were utilized. Male Ross 308 chickens received a basal diet for the first seven days and then fed with crude protein that was reduced to 194 g/kg in grower experimental diets supplemented with glutamine, glycine and additional arginine at 10, 10 and 5 g/kg respectively. Half of the 96 individual birds were injected with DEX (0.5 mg/kg body weight) or saline on days 14, 16, 18 and 20 of age. mRNA expression for jejunum and ileum for amino acid transporters (y+LAT‐1, Bo,+AT, EAAT‐3 and CAT‐1), mechanistic genes (SGLT‐1, mTOR, IAP and FABP‐2) and pro‐inflammatory genes (MUC‐2, NF‐κB, iNOS, IL‐8 and IL‐1β) were analysed using real‐time PCR. The results showed that DEX decreased y+ LAT1 in jejunum, Bo,+AT and EAAT‐3 in ileum. Arginine increased CAT‐1 in the jejunum and ileum under DEX treatment. Through an interaction, DEX reduced IAP in jejunum of glycine and arginine supplemented group and reduced mTOR in jejunum independently. DEX reduced MUC‐2 and iNOS in jejunum and increased iNOS and IL8 in the ileum. Amino acid supplementation did not appear to ameliorate these effects; however, there were some positive effects of glycine on NF‐κB and arginine through increased CAT‐1. Mechanistic understanding of amino acid supplementation in broiler diets warrants further research particularly when dietary protein is reduced below the level tested in the present study.

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Section: Discussionmentioning

confidence: 99%

Expression of selected genes encoding mechanistic pathways, nutrient and amino acid transporters in jejunum and ileum of broiler chickens fed a reduced protein diet supplemented with arginine, glutamine and glycine under stress stimulated by dexamethasone

Barekatain

Chrystal

Gilani

et al. 2020

Animal Physiology Nutrition

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show abstract

“…Es fundamental enfatizar la importancia de los estudios de prevalencia de especies para el desarrollo de vacunas regionales, como en este estudio. Se ha analizado el uso de antimicrobianos con miras a reemplazarlos para evitar el problema de resistencias y se han propuesto varias alternativas (Kim et al, 2017;Bortoluzzi et al, 2019;Lu et al, 2019;Park et al, 2020). La baja cantidad de ooquistes presentes en las muestras de este estudio (puntuación 1) y las pocas observaciones de la puntuación cero (sin ooquistes/muestra) pueden indicar la eficacia del uso estratégico de anticoccidiales químicos a través de piensos.…”

Section: Discussionunclassified

Coccidiosis en pollos de engorda comerciales en Brazil entre 2012 y 2019: especies principales y grados de daño

Gazoni¹,

Matte²,

Chiarelli-Adorno³

et al. 2021

Abanico Vet.

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El propósito del presente estudio fue determinar la frecuencia de la coccidiosis en pollos de engorde, detectar las especies más comunes de Eimeria spp y cargas parasitarias en aves comerciales en Brasil de 2012 a 2019. Estudiamos 13,648 aves entre 9 y 49 días de edad en 82 empresas en 13 estados brasileños. Las aves se seleccionaron al azar (3 a 6 aves / aviario) y se sacrificaron por eutanasia. Se analizaron las lesiones macroscópicas y se raspó la mucosa intestinal para el conteo de ooquistes de E. maxima. La clasificación de especies se basó en el tamaño de los ooquistes. También cuantificamos la carga infecciosa mediante un sistema de puntuación. Se detectó coccidiosis subclínica (E. maxima micro) en un promedio de 34.8%. E. acervulina (16.1%), E. maxima (7.9%) y E. tenella (4.1%) también se identificaron con fluctuaciones anuales. El conteo de ooquistes estuvo dentro de la puntuación 1 (1 a 10 ooquistes / ave) en el 47.5 al 84.4% de los casos; seguido de una puntuación 2 (11 a 20), 3 (21 a 40) y 4 (más de 41 ooquistes / ave). La coccidiosis subclínica es motivo de preocupación, ya que 3.5 de cada diez aves estaban infectadas. Este es un factor responsable del menor rendimiento productivo de los pollos de engorde.

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“…The requirements for BCAA may change during such states, not only for the repair of the intestinal integrity but also for the proliferation of immune cells and antimicrobial secretions. Because enteric infections increase the endogenous loss of AA, and supplemental AA are found to be essential to improve intestinal integrity and gut permeability ( Bortoluzzi et al., 2020 ; Castro et al., 2020 ), further research with BCAA is warranted to understand how these AA requirements change especially in laying and meat-type chickens and turkeys. Regarding the utilization of BCAA during poultry disease state, these are among the AA with the greatest reduction in the AID during Eimeria infection ( Parker et al., 2007 ).…”

Section: Future Bcaa Poultry Researchmentioning

confidence: 99%

Functional role of branched chain amino acids in poultry: a review

et al. 2022

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Effects of dietary amino acids in ameliorating intestinal function during enteric challenges in broiler chickens

Cited by 18 publications

References 112 publications

Expression of selected genes encoding mechanistic pathways, nutrient and amino acid transporters in jejunum and ileum of broiler chickens fed a reduced protein diet supplemented with arginine, glutamine and glycine under stress stimulated by dexamethasone

Expression of selected genes encoding mechanistic pathways, nutrient and amino acid transporters in jejunum and ileum of broiler chickens fed a reduced protein diet supplemented with arginine, glutamine and glycine under stress stimulated by dexamethasone

Coccidiosis en pollos de engorda comerciales en Brazil entre 2012 y 2019: especies principales y grados de daño

Functional role of branched chain amino acids in poultry: a review

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