Effects of diagnostic ultrasound on HSP70 expression in chorionic villi in rats during early pregnancy and the role of HSP70 in apoptosis in chorionic villi

Abstract

Abstract. The aim of this study was to investigate the effects of diagnostic ultrasound on chorionic villi during early pregnancy in rats through the examination of heat shock protein 70 (HSP70) expression and apoptosis in chorionic villi. Using TUNEL, RT-PCR, western blot analysis and immunohistochemistry, the extent of apoptosis and the gene/ protein expression of HSP70, Bcl-2 and Bax was determined in the samples of the chorionic villus from the rats during early pregnancy. Compared with the unexposed group, there were clear signs that apoptosis had occurred in the villi exposed to ultrasound for 30 min. The expression of HSP70 and Bax in the villi gradually increased with the extended duration of exposure to ultrasound, whereas the expression of Bcl-2 gradually decreased. Bcl-2 expression did not differ significantly between the group exposed for 10 min and the group exposed for 20 min. Taken together, our data demonstrate that HSP70 may protect chorionic villi exposed to ultrasound by inhibiting apoptosis; however, its protective effects are limited over the extended duration of exposure to ultrasound. Thus, the prudent use of ultrasound during the early stages of pregnancy should be advocated.

Effect of transvaginal ultrasound on human chorionic villus cell apoptosis during pregnancy

“…The results suggest that ultrasound exposure may have an effect on cell apoptosis. This is consistent with previous studies where morphological alternations in chorionic cells were observed after 5-min high frequency transvaginal ultrasound or 20-min abdominal ultrasound exposure (Liu et al, 2013). When cells were exposed to 10-min transvaginal high frequency ultrasound, apoptosis increased as well (Ratto et al, 2011), which is consistent with our study results.…”

Rekombinan Vascular Endothelial Growth Factor-121 Menekan Ekspresi Heat Shock Protein-70 pada Plasenta Mencit Model Preeklampsia (VASCULAR ENDOTHELIAL GROWTH FACTOR-121 RECOMBINANT DEPRESSIS HEAT SHOCK PROTEIN-70 EXPRESSION IN PREECLAMPSIA MICE PLACENTA

“…Senyawa VEGF-121 rekombinan yaitu VEGF eksogen, yang merupakan protein proangiogenik yang berperan terhadap proses vaskulogenensis dan angiogenesis, mempunyai reseptor di dinding endotel yaitu vascular endotelial growth faktor receptore-1 (VEGFR-1), dan VEGFR-1 dapat terikat lebih kuat terhadap sFlt-1. Pemberian VEGF rekombinan 121 menurunkan hipertensi dan memperbaiki lesi di renal pada binatang model preeklampsia yang diberi sFlt-1 berlebihan (Liu et al, 2013). Senyawa VEGF mempunyai efek secara langsung terhadap vasculature sistemik.…”