Effects of Diacetyl Flavoring Exposure in Mice Metabolism

Jedlicka, Letícia Dias Lima; Silva, Juciara da Costa; Balbino, Aleksandro Martins; Neto, Giuseppe Bruno; Furtado, Danielle Zildeana Sousa; Silva, Heron Dominguez Torres da; Cavalcanti, Fernanda de Barros Correia; Heijden, Karin Marie van der; Penatti, Carlos A. A.; Bechara, Etelvino José Henriques; Assunção, Nilson Antônio

doi:10.1155/2018/9875319

Cited by 9 publications

(4 citation statements)

References 56 publications

(60 reference statements)

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“…Ageand sex-matched wild type C57BL/6 mice were employed as negative controls. The selection of an in vivo dose of DA was based on our initial toxicity studies and literature reports 27 confirming safety of a 100 mg/kg dose in mice. The behavior of these mice was observed after three months of DA treatment at the age of 7-8 months.…”

Section: ■ Resultsmentioning

confidence: 99%

The Amyloid Aggregation Accelerator Diacetyl Prevents Cognitive Decline in Alzheimer’s Mouse Models

Xie

Kim

Vince

et al. 2021

Chem. Res. Toxicol.

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Diacetyl (DA), a food flavorant, is linked with occupational lung disease. Our in vitro experiments described the formation of a covalent adduct by DA with Arg 5 of the Aβ 1−42 peptide, which resulted in only a transient increase in neurotoxicity in SH-SY5Y cells. However, in vivo implications of these effects on Alzheimer's disease (AD) pathogenesis and the underlying mechanisms remain poorly understood. In the APP/PS1 transgenic AD mouse model, DA treatment did not exacerbate learning and memory deficits in the Morris water maze test. Moreover, DA increased the Aβ 1−42 plaque burden and decreased neuronal inflammation in the transgenic AD mice. Additionally, cognitive impairment induced by intracerebroventricular Aβ 1−42 was restored by the DA treatment, as assessed by the T-maze test. A corresponding mitigation of neuronal inflammation was also observed in the hippocampus of these nontransgenic mice due to the acceleration of Aβ 1−42 aggregation by DA into nontoxic plaques. The data from SDS-PAGE, dot-blot, and TEM in vitro experiments corroborated the acceleration of the Aβ 1−42 aggregation observed in vivo in AD animal models and characterized the DA-induced formation of Aβ 1−42 fibrils. Such Aβ 1−42 −DA fibrils were unstable in the presence of detergent and amenable to detection by the thioflavin T reagent, thus underscoring the distinct assembly of these fibrils compared to that of the fibrils of the native Aβ 1−42 . Taken together, the results of this study present for the first time the in vivo implications of the DA-induced acceleration of Aβ 1−42 and may provide a strategy for the rational design of Aβ 1−42 aggregation accelerators as AD therapeutics that promote oligomer-free Aβ 1−42 fibril formation.

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Section: ■ Resultsmentioning

confidence: 99%

The Amyloid Aggregation Accelerator Diacetyl Prevents Cognitive Decline in Alzheimer’s Mouse Models

Xie

Kim

Vince

et al. 2021

Chem. Res. Toxicol.

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“…It is the main flavor of cream, and can also be used in milk, cheese, and other fragrances (Sirois et al, 2018, Fechter-Leggett et al, 2018). It can also be used in cosmetic fresh fruit flavors and as gelatin hardener and photographic binder (Jedlicka et al, 2018).…”

Section: Results and Analysismentioning

confidence: 99%

Pyrolysis molecule of Torreya grandis bark for potential biomedicine

Chen

Yue

Yang

et al. 2019

Saudi Journal of Biological Sciences

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Torreya grandis is a unique tree species in China. Although full use has been made of the timber, the processing and utilization of the bark has not been effective. In order to explore a new way to utilize the bark of Torreya grandis , a powder of T. grandis bark was prepared and analyzed qualitatively and quantitatively. Differential scanning calorimetry (TG) and pyrolysis gas chromatography-mass spectrometry (PY-GC/MS) revealed many bioactive components in the bark of T. grandis, such as acetic acid, 2-methoxy-4-vinyl phenol, D-mannose, and furfural. These substances have potential broad applications in the chemical industry, biomedicine, and food additives. The chemical constituents of the bark of T. grandis suggest a theoretical basis for the future development and utilization of the bark of T. grandis .

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“…Mice were left to acclimate in laboratory environment with free access to food and water for one week before the onset of the experiment. Light-dark photoperiod was maintained at 12:12 h in a controlled environment temperature of 25±2 °C and a relative humidity of 40±5% according to (Jedlicka et al 2018).…”

Section: Animalsmentioning

confidence: 99%

Diacetyl and Butter Flavors Induces Genotoxic Effects and Liver Toxicity in Male Swiss Albino Mice after Acute and Chronic Exposure.

Aboelmaaty

Fahmy

Ebeed

et al. 2021

Arab Universities Journal of Agricultural Sciences

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Since the mid-1950s, the volatile structure of butter oil and butter were researched, and an exhaustive list of elements has been collected. Diacetyl is an aromatic popular synthetic fragrance that gives food a buttery taste used in ice cream, snacks and potting with butter, strawberry, caramel, or cheese flavor. The chromosomal aberrations and micronuclei are commonly used biomarkers of chromosomal damage, genome stability, and cancer risk assessment. In vivo trials are still important to assess the genetic toxicology of chemical products such as industrial chemicals, pharmaceuticals, and food additives. This study aimed at assessing the potential genotoxic effect of diacetyl and butter flavors on swiss albino mice using alterations in liver function enzymes, micronucleus (MN), and chromosomal aberrations (CA) assays. The results showed that exposure of swiss albino mice males to diacetyl and butter flavors induced (CA) and (MN) in a statistically highly significant manner compared to the control. Meanwhile, the biochemical analysis revealed that these substances caused an exceptional rise in liver function enzymes (AST, ALT, and ALP) activity in serum of treated experimental animals. In conclusion, both tested compounds have increased the chromosomal aberration, micronucleus test, and serum levels of liver function enzymes indicating their high potential of being cytotoxic and genotoxic materials.

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Effects of Diacetyl Flavoring Exposure in Mice Metabolism

Cited by 9 publications

References 56 publications

The Amyloid Aggregation Accelerator Diacetyl Prevents Cognitive Decline in Alzheimer’s Mouse Models

The Amyloid Aggregation Accelerator Diacetyl Prevents Cognitive Decline in Alzheimer’s Mouse Models

Pyrolysis molecule of Torreya grandis bark for potential biomedicine

Diacetyl and Butter Flavors Induces Genotoxic Effects and Liver Toxicity in Male Swiss Albino Mice after Acute and Chronic Exposure.

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