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According to their special physio-chemical properties, Nanoparticles have gained worldwide attention as a new bio-alternative for chemical control agents. This investigation aims to the eco-friendly synthesis of nanosilver particles from tomato peel extract (TPE-AgNPs) and evaluates their characteristics and inhibitory activities against pathogenic bacteria and fungi as well as their role in metallic surface disinfecting. To initiate biosynthesis, tomato peel extract was mixed with silver nitrate (AgNO3) solution until the color changes to reddish brown. Ultraviolet (UV-Visible) spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) analysis, and transmission electron microscopy (TEM) techniques were used to characterize biosynthesized TPE-AgNPs. Results recorded that obtained TPE-AgNPs had a strong score (238nm) of Plasmon resonance (SPR) by SPR of 4.5. Functional groups of carboxyl, hydroxyl, and phenolic groups existed and were detected by the FTIR spectrum. The synthesized TPE-AgNPs had an amorphous nature which was confirmed by XRD analysis. TEM analysis showed spherical TPE-AgNPs sized from 4.44-27.59nm. The biosynthesized TPE-AgNPs had a negative zeta potential of -68.44 mV. The inhibitory activities of synthesized TPE-AgNPs were evaluated against eleven microbial pathogenic using well diffusion method, inhibition zone diameter (IZD) was measured in centimeters. Results showed that B. subtilis and E.coli was the most sensitive pathogens with IZD of 4.0 and 0.92cm, respectively However, L. monocytogenes and S. sonnei were the most resistant pathogens with IZD of 0.92 and 0.90 cm, respectively. Synthesized TPE-AgNPs from tomato peels had good inhibitory potentials against pathogenic fungi with IZD of 3.0 and 0.92cm against A. solani and C. albicans, respectively. Applying the use of TPE-AgNPs as bio disinfectant significantly decreased the microbial load of metallic blades and proves its efficiency as a disinfectant agent after 120min. of contacting. So, more applications on disinfecting metallic surfaces such as dentistry are indeed needed.

Artemisia (Asteraceae family) has been reported to possess different bioactive phytochemicals including phenols, sesquiterpene lactones and flavonoids. The aim of this study was to estimate the anti-proliferative activity of the methanolic extract of Artemisia herba alba using in vitro assays on two of human cancer cell lines compared with the normal cell lines. The obtained results indicated that A.herba alba extract showed highly cytotoxic and anti-proliferative effects on tested human cancer cells as revealed by measuring viable mitochondrial oxidation of [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay] (MTT assay). The observed (-IC50-) or the half-maximal inhibitory concentration of A. herba alba extract in the human hepatocellular carcinoma (HepG2) was 50.34 μg/ml, while in the lung cancer cell line (A549) it was 29.23 μg/ml. In contrast, the normal liver cells (THLE2) as well as lung normal cells showed very low sensitivity toward tested extract where the IC50 were 1250μg/ml and 1915 μg/ml, respectively. However, flow cytometric analysis for cell cycle using propidium iodide staining revealed increment of G2/M phase cell cycle arrest after treatment of HepG2 cells with A. herba alba extract. At the molecular level, the quantitative real time-PCR technique was used to investigate the alteration of gene expression after exposure of HepG2 cell line to A. herba alba extract by measuring levels of mRNA for p53, Bax, and Bcl-2 genes expression. The apoptotic mechanism was activated by the crude extract of A. herba alba extract included up-regulation of p53 and Bax and downregulation of Bcl-2 expression levels. However, no cytotoxic effect was recorded after exposure of normal cell lines to the plant extract. These data indicated that A. herba alba extract possess antiproliferative effect by a cell cycle arrest at the G2/M phase and apoptosis mediated cytotoxicity in carcinoma cells. These results suggested that A.herba alba could contain one or more effective anti-cancer compound(s) and can be used as a natural source of anticancer agents.

Since the mid-1950s, the volatile structure of butter oil and butter were researched, and an exhaustive list of elements has been collected. Diacetyl is an aromatic popular synthetic fragrance that gives food a buttery taste used in ice cream, snacks and potting with butter, strawberry, caramel, or cheese flavor. The chromosomal aberrations and micronuclei are commonly used biomarkers of chromosomal damage, genome stability, and cancer risk assessment. In vivo trials are still important to assess the genetic toxicology of chemical products such as industrial chemicals, pharmaceuticals, and food additives. This study aimed at assessing the potential genotoxic effect of diacetyl and butter flavors on swiss albino mice using alterations in liver function enzymes, micronucleus (MN), and chromosomal aberrations (CA) assays. The results showed that exposure of swiss albino mice males to diacetyl and butter flavors induced (CA) and (MN) in a statistically highly significant manner compared to the control. Meanwhile, the biochemical analysis revealed that these substances caused an exceptional rise in liver function enzymes (AST, ALT, and ALP) activity in serum of treated experimental animals. In conclusion, both tested compounds have increased the chromosomal aberration, micronucleus test, and serum levels of liver function enzymes indicating their high potential of being cytotoxic and genotoxic materials.

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