Diacetyl is a flavoring that imparts a buttery flavor to foods, but the use or exposure to diacetyl has been related to some diseases. We investigated the effect of oral intake of diacetyl in male and female C57/Bl mice. We performed a target metabolomics assay using ultraperformance liquid chromatography paired with triple quadrupole mass spectrometry (UPLC-MS/MS) for the determination and quantification of plasmatic metabolites. We observed alterations in metabolites present in the urea and tricarboxylic acid (TCA) cycles. Peroxynitrite plasmatic levels were evaluated by a colorimetric method, final activity of superoxide dismutase (SOD) was evaluated by an enzymatic method, and mouse behavior was evaluated. Majority of the assay showed differences between control and treatment groups, as well as between genders. This may indicate the involvement of sex hormones in the regulation of a normal metabolic profile, and the implication of sex differences in metabolite disease response.

“Introdução à Genética Forense” é uma bibliografia organizada através do empenho de 5 profissionais da perícia científica.  A obra dispõe da colaboração de 27 autores gabaritos nas mais distintas áreas entre elas: ciências biológicas, ciências biomédicas, odontologia e ciências farmacêuticas. O linear de intersecção entre eles é a ampla experiência nas distintas esferas da genética forense.