Effects of compressed carbon dioxide treatment on the specificity of oxidase enzymatic complexes from mate tea leaves

Primo, M.S.; Ceni, Giovana Cristina; Marcon, Naiane Sabedot; Antunes, O. A. C.; Oliveira, Débora de; Oliveira, J. Vladimir; Dariva, Cláudio

doi:10.1016/j.supflu.2007.07.004

Cited by 25 publications

(14 citation statements)

References 43 publications

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“…Compared with the untreated sample, POD in carrot slices after treatment by HPCD was activated at these three pressure levels by 9.3-59.1%. Similar results were reported by Primo et al (2007), who found an enhancement of around 25% in POD activity from mate tea leaves extracts treated at 7.05 MPa and 30°C for 1 h. The initial increase in POD activity might be the result of a defense mechanism, since POD activity in plants increases in response to various biotic and abiotic stresses (Kwak, Kim, Park, & Liu, 1996). The only marked reduction of POD activity in carrot slices was at 5 MPa for 15 min by 24.2% ( Fig.…”

Section: Effect On Enzymessupporting

confidence: 87%

High pressure carbon dioxide treatment for fresh-cut carrot slices

Zhang

et al. 2011

Innovative Food Science & Emerging Technologies

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Section: Effect On Enzymessupporting

confidence: 87%

High pressure carbon dioxide treatment for fresh-cut carrot slices

Zhang

et al. 2011

Innovative Food Science & Emerging Technologies

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“…According to many authors, supercritical carbon dioxide could remove the essential water responsible for maintaining the enzymatic activity (Fricks et al, 2006;Oliveira, Feihrmann, Rubira, et al, 2006;Primo et al, 2007). Conversely, in this work, we verified an increase in the enzyme activity after treatment with Table 3 Matrix of the CCD (real and coded values) with responses in terms of the b-galactosidase activity submitted to compressed nbutane.…”

Section: Effect Of Compressed N-butane On the B-galactosidase Activitsupporting

confidence: 67%

Effect of compressed fluids treatment on the activity, stability and enzymatic reaction performance of β-galactosidase

et al. 2011

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a b s t r a c tThe present work investigates, as a first step, the influences of pressure, exposure time and depressurisation rate on the b-galactosidase activity of permeabilized cells of Kluyveromyces marxianus CCT 7082 submitted to treatment with compressed carbon dioxide, propane and n-butane. In general, results showed that the enzyme activity changes significantly, depending on the experimental conditions investigated, allowing the selection of proper operating conditions for advantageous application of this biocatalyst in hydrolysis reactions. Further, the stability of the enzyme, after high-pressure pre-treatment, was also experimentally monitored, and the results demonstrated that the activities of this biocatalyst were always higher than those of the non-treated one. Finally, evaluation of synthesis of galactooligosaccharides (GOS), using b-galactosidase without treatment and after treatment in compressed fluids, was carried out. Results indicated promising possibilities since the use of n-butane-pre-treated enzyme led to very satisfactory reaction conversions and selectivity.

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“…According to many authors, supercritical carbon dioxide could remove the essential water responsible for keeping the enzymatic activity (Primo et al 2007;Oliveira et al 2006a;Oliveira et al 2006b;Fricks et al 2006).…”

Section: Lyophilized Lipase Amano Psmentioning

confidence: 99%

Effect of Treatment with Compressed Propane on Lipases Hydrolytic Activity

Franken

Marcon

Treichel

et al. 2008

Food Bioprocess Technol

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The objective of this work was to assess the influence of compressed propane treatment on the hydrolytic activity of three lipases (Amano PS, Amano AY30, and a non-commercial lipase from Yarrowia lipolytica) free, resuspended, and in immobilized form. To evaluate the effect of process variables on the lipase activity, a semifactorial experimental design with two levels and four variables was employed for free and immobilized lipases and a full 2 2 experimental design was carried out for lipases in solution. The residual activity was defined as the ratio of lipase activity before and after treatment with pressurized propane. For free and immobilized lipases, an enhancement in residual lipase activity in most of the experimental conditions investigated was observed. In the case of resuspended lipases, it is shown that enzyme kinetics is sensitive to treatment with compressed propane resulting in remarkable gains and losses of enzyme activity. In a general way, the results showed that the enzyme activity changes significantly depending on the enzyme, the presentation form, and the experimental conditions investigated, allowing the selection of operational conditions in terms of temperature, pressure, exposure time, and depressurization rate for advantageous application of these biocatalysts in hydrolysis reactions.

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Effects of compressed carbon dioxide treatment on the specificity of oxidase enzymatic complexes from mate tea leaves

Cited by 25 publications

References 43 publications

High pressure carbon dioxide treatment for fresh-cut carrot slices

High pressure carbon dioxide treatment for fresh-cut carrot slices

Effect of compressed fluids treatment on the activity, stability and enzymatic reaction performance of β-galactosidase

Effect of Treatment with Compressed Propane on Lipases Hydrolytic Activity

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