Effects of commercial aldehydes from green leaf volatiles (green odour) on rumen microbial population and fermentation profile in an artificial rumen (Rusitec)

Demirtaş, Ahu; Öztürk, Hakan; Sudağıdan, Mert; Keyvan, Erhan; Yavuz, Orhan; Gülay, Özlem Yıldız; Musa, Saad Ahmed Adam

doi:10.1016/j.anaerobe.2018.11.001

Cited by 12 publications

(8 citation statements)

References 65 publications

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“…The ruminal samples were allowed to thaw completely at 4°C before the analysis. The VFA and lactate concentrations were quantified by HPLC as described previously (14). The NH3-N concentration was determined with indophenol blue method using the spectrophotometer (UV-150-02; Shimadzu) at 546 nm (9).…”

Section: Methodsmentioning

confidence: 99%

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Effects of Urtica dioica, Matricaria chamomilla, and Vitex agnus-castus extracts on in vitro rumen fermentation in normal and following acidosis conditions

Demirtaş

Pişkin

2019

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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The aim of this study was to investigate the effects of dry extracts of Urtica dioica, Matricaria chamomilla, and Vitex agnus-castus with high phenolic contents on rumen microbial fermentation as compared with those of monensin, a common ionophore antibiotic, using Rumen Simulation Technique (RUSITEC) under normal and acidosis conditions. The treatments were as follows: negative control (no additive), positive control (5 mg/d monensin), and extracts of U. dioica (500 mg/d), M. chamomilla (500 mg/d), and V. agnus-castus (500 mg/d). Neither the plant extracts nor monensin altered the ruminal pH under normal or acidosis conditions. All the treatments affected total volatile fatty acid (VFA) production, propionate production, and dry matter digestibility (DMD), regardless of the fermentation conditions. All three extracts increased (P<0.05) total VFA production similar to that observed with monensin (P<0.05). M. chamomilla and V. agnus-castus increased propionate production and DMD similar to that obtained with monensin (P<0.05). In contrast to the monensin treatment, all three extracts increased acetate production under normal conditions (P<0.05). Under acidosis conditions, acetate production remained unchanged in the U. dioica and V. agnus-castus treatments, as well as in the monensin treatment. Under both conditions, the acetate-to-propionate (A:P) ratio decreased only in the monensin treatment (P<0.05). U. dioica and M. chamomilla had antiprotozoal effects (P<0.05) similar to those of monensin, regardless of the condition. The NH3-N concentration declined only in the V. agnus-castus treatment under acidosis conditions (P<0.05). Similar to the monensin treatment, lactate concentrations remained unchanged in the V. agnus-castus treatment under both conditions. In conclusion, plant extracts stimulated fermentative activity of rumen microorganisms under normal and acidosis conditions. Although they did not improve ruminal pH, U. dioica and V. agnus-castus extracts had more favorable effects on some fermentation parameters under acidosis conditions.

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Section: Methodsmentioning

confidence: 99%

“…For protozoa counting, rumen fluid samples were removed from the fermenters daily immediately before substrate exchange. The total number of protozoa was counted as described by Demirtas et al (14).…”

Section: Methodsmentioning

confidence: 99%

Effects of Urtica dioica, Matricaria chamomilla, and Vitex agnus-castus extracts on in vitro rumen fermentation in normal and following acidosis conditions

Demirtaş

Pişkin

2019

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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“…Green leaf volatile aldehydes moderately promote the growth of some beneficial gut bacteria like B. bifidum and L. acidophilus at lower concentrations. In a previous study, low concentrations of trans-2-hexenal and trans-2-decenal also stimulated the growth of pure cultures of Fibrobacter succinogenes, which contribute to pre-gastric cellulose fermentation in ruminants (13). Saponins, one of the phytochemicals, promoted in vitro bacterial growth and feed utilization in the rumen at low doses while they exhibited inhibition at high doses (30).…”

Section: Resultsmentioning

confidence: 85%

“…However, bacteriostatic activity of aldehyde compounds was greater than that of the alcohol ones (27). In a previous study, we observed that cis-3-hexenal increased the abundance of some Grampositive anaerobic fermenters, such as Butyrivibrio fibrisolvens and Streptecoccus bovis, in a simulated rumen (13). Although there are a few reports about the effects of some green-leaf-derived compounds on some agents of gastrointestinal tract infections (4,21,27), the effects of green leaf volatiles on beneficial gut bacteria have not been evaluated previously.…”

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confidence: 70%

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Preliminary study on stimulatory and inhibitory effects of aldehydes from the green leaf volatiles family on beneficial and pathogenic bacteria from the intestine

Goker¹,

Demirtaş²

2020

Medycyna Weterynaryjna

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The balance between beneficial and pathogenic bacteria in the intestine has a great importance in terms of gut physiology and immunity. The aim of the study was to investigate the stimulatory and inhibitory effects of aldehydes from the green leaf volatiles family (trans-2-hexenal, cis-3-hexenal, trans-2-nonenal, and trans-2- decenal) on beneficial (Bifidobacterium bifidum, Bifidobacterium longum subsp. infantis, Lactobacillus acidophilus, and Lactobacillus casei) and pathogenic bacteria (Fusobacterium nucleatum subsp. nucleatum, Clostridium perfringens, Staphylococcus aureus subsp. aureus, Escherichia coli, and Salmonella enterica subsp. enterica serovar Typhimurium) from the intestine. The growth of B. bifidum was stimulated by trans-2-hexenal and trans-2-decenal at 3.9-250 μg/mL, by cis-3-hexenal at 15.6 and 31.3 μg/mL, and by trans-2-nonenal at a dose of 3.9-500 μg/mL (p < 0.05). Trans-2-decenal also moderately stimulated L. acidophilus at concentrations of 31.3 and 62.5 (p < 0.05). Trans-2-hexenal, cis-3-hexenal, and trans-2-nonenal did not inhibit beneficial intestinal bacteria, with the exception of B. infantis. Trans-2-decenal was the most effective aldehyde on pathogens, with growth-inhibitory effect on C. perfringens, F. nucleatum, and S. aureus at the concentration of 500 μg/mL. Trans-2-decenal also protected beneficial bacteria at the dose at which it inhibited pathogenic ones. All the used aldehydes at a concentration of 500 μg/mL inhibited the growth of F. nucleatum as one of the agents of colorectal cancer. Among the pathogens, E. coli and S. Typhimurium were resistant to all aldehydes while S. aureus was inhibited only by trans-2-decenal. In conclusion, the use of aldehydes from the green leaf volatiles family might have beneficial effects on gut health by regulating beneficial bacteria and inhibiting pathogenic bacteria.

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Potentials of patchouli (Pogostemon cablin) essential oil on ruminal methanogenesis, feed degradability, and enzyme activities in vitro

El-Zaiat

Abdalla

2019

Environ Sci Pollut Res

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Effects of commercial aldehydes from green leaf volatiles (green odour) on rumen microbial population and fermentation profile in an artificial rumen (Rusitec)

Cited by 12 publications

References 65 publications

Effects of Urtica dioica, Matricaria chamomilla, and Vitex agnus-castus extracts on in vitro rumen fermentation in normal and following acidosis conditions

Effects of Urtica dioica, Matricaria chamomilla, and Vitex agnus-castus extracts on in vitro rumen fermentation in normal and following acidosis conditions

Preliminary study on stimulatory and inhibitory effects of aldehydes from the green leaf volatiles family on beneficial and pathogenic bacteria from the intestine

Potentials of patchouli (Pogostemon cablin) essential oil on ruminal methanogenesis, feed degradability, and enzyme activities in vitro

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