Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage

Effects of shoot tip colchicine applications on some grape cultivars

“…Sekiguchi et al (1971) indicated that shoots did not grow as a result of the damage to the shoot tip area due to applications with mutagenic effects in some species. Also, it was reported that the shoot tips dyed at varying rates in antimitotic applications made to different types of tissues in rootstocks and grape cultivars due to the toxic effect of the chemical used (He et al, 2016;Kara et al, 2018). The findings from the present study are similar to these results.…”

“…Two weeks after the applications, the number of surviving shoot tips was determined by proportioning the number of alive shoot tips to all shoots of treated plants (%) (Kara et al, 2018).…”

“…In order to meet the pollen needs of 'Ekşi Kara' (Kara et al, 2017b), and to increase its marketability without loss of adaptation ability to the area where traditional cultivar is grown, its fruit quality characteristics must be improved (Kara et al, 2017b). Previous studies conducted on chromosome doubling differ in terms of application doses, durations, tissue types, and application methods for polyploidy stimulation (Değirmenci-Karataş et al, 2010;Dhooghe et al, 2011;Kuliev, 2011;Ma et al, 2014;Kara et al, 2018). Studies are needed to determine the proper colchicine application methods with different cultivars, and to obtain cultivars with increased ploidy levels.…”

Polyploidy in tissues of plants in vitro of grape somaclones

“…Recently, an indirect method for assessing plant ploidy has been widely used, based on the revealed relationships of genome ploidy with morphological features of the leaf epidermis [8,9,10]. In this work, the significant direct correlation was found between the frequency of polyploidy in meristem tissues of in vitro plants and the number of chloroplasts in the stomata of grape somaclones.…”

“…It is necessary to take into account when choosing the method of polyploidization that the effectiveness of the action of an antimitotic agent depends on its concentration, duration of exposure, genotype of the original form, germination and survival of seeds [7]. During the study of the possibility of polyploidy induction in grapes by treating the apical meristem with colchicine, it turned out that the tested genotypes practically did not respond to the effect of colchicine in the direction of genome doubling [8]. The results of the experiment with hybrid progeny of Muscadinia rotundifolia, Vitis riparia and Vitis rupestris, obtained using various combinations of explants, antimitotic agents, their exposure time and concentration, allowed to create an effective protocol for the induction of in vitro tetraploids for rootstock breeding programs [9].…”