Effects of Calendula officinalis on human gingival fibroblasts

Saini, Pragtipal; Al‐Shibani, Nouf; Sun, Junliang; Zhang, Weiping; Song, Feifei; Gregson, Karen S.; Windsor, L. Jack

doi:10.1016/j.homp.2012.02.003

Cited by 103 publications

(17 citation statements)

References 26 publications

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“…Despite the lack of studies evaluating the effect of the crude extract of CLO on bone tissue, it has been reported that queretin, the main flavonoid found on CLO extract, is the one responsible, in great part, of the pharmacological effects of this plant (Saini et al, 2012). In vitro , quercetin significantly increased osteoblast differentiation (Zhou et al, 2015) and induced mRNA expression of sialoprotein and osteocalcin in osteoblast culture (Satué et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…CLO preserved collagen confirming others studies which already showed that CLO extract reduced collagen breakdown (Millán et al, 2016), and increased collagen concentration (Aro et al, 2015). In gingival fibroblasts, CLO inhibited matrix metalloproteinases (MMP)-2 (Saini et al, 2012). Collagen is the main constituent of periodontal ligament and plays a key role in the architecture of the periodontium (Kaku and Yamauchi, 2014).…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Calendula officinalis on Oxidative Stress and Bone Loss in Experimental Periodontitis

et al. 2017

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Periodontitis is associated with reduced antioxidant capacity and increased oxidative damage. Oxidative stress induces inflammation and bone loss contributing to the pathological progression of periodontal disease. Calendula officinalis (CLO) has demonstrated anti-inflammatory and anti-oxidant activities. Therefore, the aim of this study was to evaluate the effect of CLO on oxidative stress and bone loss in rats subjected to experimental periodontitis (EP). For this, 72 male Wistar rats were divided into groups: Naïve, Saline (SAL) and CLO. Rats received SAL or CLO (90 mg/kg) 30 min before ligature and daily until the 11th day. Naïve group experienced no manipulation. After 11 days, the animals were euthanized and left maxillae collected for macroscopic analysis of alveolar bone loss (ABL). Periodontium was analyzed by macroscopy, scanning electron microscopy; confocal and light polarized microscopy. Immunohistochemical examination of DKK1, WNT 10b and β-catenin was performed. The gingival tissue was collected to reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) analyses. The 11 days of ligature induced bone loss, breakdown of collagen fibers, increased the immunostaining DKK-1 while reduced WNT 10b and β-catenin expressions. Periodontitis reduced GSH, SOD, CAT and increase MDA. All findings were reversed by 90 mg/kg of CLO. In summary our findings demonstrated that CLO reduced oxidative stress and bone loss and preserved collagen fibers in rats with EP, with participation of WNT signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Effect of Calendula officinalis on Oxidative Stress and Bone Loss in Experimental Periodontitis

et al. 2017

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“…The flowers of C. officinalis are well known for their anti-inflammatory, antipyretic, antitumor, and healing effects (Saini et al 2012;Bortoldo et al 2009) as well as antioxidant activity (Shivasharan et al 2013). These activities are related to the chemical composition of the plant, especially the presence of flavonoids and polyphenols (Butnariu & Coradini 2012;Borella et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Polysaccharides isolated from Calendula officinalis ethanolic fraction have shown antitumor activity (Parente et al 2004). The presences of lutein, lycopene, and xanthophylls, which contribute to anti-oxidant and anti-inflammatory effects, have also been described (Saini et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical evaluation and in vitro antioxidant and photo-protective capacity of Calendula officinalis L. leaves

Deuschle

Piana

et al. 2015

Rev. bras. plantas med.

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ABSTRACT:The plant Calendula officinalis L. is widely applied due to its medicinal properties, which are mainly dermatological and ornamental. The goal of this study is to assess the phytochemical components in a hydroethanolic extract (HECO) from the leaves of Calendula officinalis L. using UV-VIS spectrophotometry and thin layer chromatography (TLC), as well as to identify and quantify the components related to its antioxidant capacity employing high performance liquid chromatographic (HPLC). The antioxidant capacity evaluation was performed using the DPPH method for superoxide and hydroxyl radicals. The photo-protective capacity was evaluated by UVspectrophotometry in order to determine the in vitro Sun Protection Factor(SPF). The results show the plant's strong antioxidant activity (DPPH and hydroxyl methods), which we believe to be related to the presence of flavonoids (24.67 mg/g), polyphenols (33.90 mg/g), condensed tannins (27.30 mg/g), and the amount of rutin (37.25 mg/g), and quercetin (6.09 mg/g) found during the study. The HECO presented a good antioxidant capacity, most likely due to the polyphenols, flavonoids, and tannins in its contents. However, the obtained SPF of 1.89 ± 0.05 does not allow the plant to be classified as a stand-alone sunscreen, and more studies are needed in order to test its ability to enhance sunscreens in existing cosmetic formulations.Keywords: Calendula officinalis. Marigold. Antioxidant. Phenolic Compounds. Photo-protection. RESUMO:Avaliação fitoquímica e capacidade antioxidante e fotoprotetora in vitro das folhas de Calendula officinalis L. A Calendula officinalis L. é uma planta amplamente utilizada por suas propriedades medicinais, principalmente dermatológica e ornamental. O objetivo deste estudo é avaliar constituintes fitoquímicos do extrato hidroetanólico das folhas de Calêndula officinalis L. (HECO) por espectrofotometria UV-visível e cromatografia em camada delgada (CCD), bem como detectar e quantificar os componentes relacionados com a capacidade antioxidante por Cromatografia Líquida de Alta Eficiência (CLAE). Posteriormente, foi avaliada a capacidade antioxidante pelo método do DPPH, radicais superóxido e hidroxila. Além disso, a capacidade fotoprotetora foi avaliada através de espectrofotometria UV para determinação do Fator de Proteção Solar in vitro (FPS). Os resultados evidenciaram que a planta apresentou uma excelente atividade antioxidante para o método do DPPH e do radical hidroxila, o que pode estar relacionada com a presença de flavonoides (24,67 mg/g), polifenóis (33,90 mg/g) e taninos condensados (27,30 mg/g) e da quantidade de rutina (37,25 mg/g) e quercetina (6,09 mg/g) encontrados neste estudo. O HECO apresentou uma notável capacidade antioxidante, provavelmente devido à presença de polifenóis, flavonoides e taninos. O valor do FPS encontrado (1,89 ± 0,05), não classifica a planta como um protetor solar isolado. No entanto, mais estudos são necessários para testar a capacidade de potencializar filtros solares em formulações cosméticas.

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“…Junto a estos fibroblastos y otras células de defensa, se producen citocinas pro-inflamatorias (Ara et al 2013, Baek et al 2013) como la Interleucina 1ß (IL 1ß; Bhawal et al 2012, Sawada et al 2013, Factor de Necrosis Tumoral alfa (TNFα; Guimarães et al 2011, Arancibia et al 2013) y citocinas anti-inflamatorias como lainterleucina10 (IL-10); que controla una respuesta inflamatoria exacerbada (Morandini et al 2011, Gómez-Florit et al 2013. De esta forma, la regulación de las funciones de remodelación del tejido conectivo y respuesta inflamatoria de los fibroblastos gingivales con medicamentos antihomotóxicos, podría tener un gran potencial terapéutico en periodoncia (Saini et al 2012). El objetivo de esta investigación, fue establecer el efecto de Mercurius-Heel®S sobre fibroblastos gingivales cultivados in vitro en diferentes tiempos y concentraciones, con el fin de dar una explicación científica al uso del Mercurius-Heel®S en enfermedades orales como la periodontitis.…”

Section: Medición De La Citotoxicidad Y Producción De Citocinas En Fiunclassified

Medición de la citotoxicidad y producción de citocinas en fibroblastos gingivales humanos estimulados con Mercurius−Heel®S: Un estudio piloto

Robayo

Patiño²,

Rueda³

2014

Univ. Sci.

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Mercurius-Heel®S is a homeopathic remedy used to treat infectious diseases such as gingivitis and periodontitis, mainly in the oral cavity. The main component of this drug is the Mercurius solubilis. Its cytotoxic effect was established based on cell viability and its anti-inflammatory effect by measuring the cytokines Mercurius-Heel®S on human gingival fibroblasts (FGH). We treated cells with Mercurius-Heel®S at concentrations of 300 mg/ml to 0.0005 mg/ml and left untreated cells as a control. Cell viability was measured using the MTS® colorimetric assay and the expression of cytokines (IL1b, TNFa e IL10) present in cell supernatants was performed with Quantiquine® ELISA. From 3.7 mg/ml to 0.0005 mg/ml, we observed a significantly higher increase in cell viability in comparison to the control (lower concentrations of the drug). Cytokine production was not significantly different at different concentrations of Mercurius-Heel®S. There was no significant increase in IL1b, IL10 and TNFa and Mercurius-Heel®S showed no cytotoxic effect on FGH.

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Effects of Calendula officinalis on human gingival fibroblasts

Abstract: Calendula inhibits HGF-mediated collagen degradation and MMP-2 activity more than the corresponding concentration of quercetin. This may be attributed to additional components in Calendula other than quercetin.

Cited by 103 publications

References 26 publications

The Effect of Calendula officinalis on Oxidative Stress and Bone Loss in Experimental Periodontitis

The Effect of Calendula officinalis on Oxidative Stress and Bone Loss in Experimental Periodontitis

Phytochemical evaluation and in vitro antioxidant and photo-protective capacity of Calendula officinalis L. leaves

Medición de la citotoxicidad y producción de citocinas en fibroblastos gingivales humanos estimulados con Mercurius−Heel®S: Un estudio piloto

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