The Effect of Calendula officinalis on Oxidative Stress and Bone Loss in Experimental Periodontitis

Lima, Mariana dos Reis; Lopes, Amanda P.; Martins, Carla Adriano; Brito, Gerly Anne Castro; Carneiro, Virgínia C.; Góes, Paula

doi:10.3389/fphys.2017.00440

Cited by 33 publications

(23 citation statements)

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“…Besides bone homeostasis, Dkk-1 may play an important role in pathological bone loss (8) since inflammatory mediators induce Dkk-1 production (9, 10). It has been previously reported by our group and others, that during periodontitis, there is an increase of Dkk-1 in the periodontal tissue (11)(12)(13). However, to date, there is no substantial evidence regarding the actual origin and role of Dkk-1 in the bone loss related to periodontitis.…”

Section: Introductionmentioning

confidence: 78%

Loss of Dkk-1 in Osteocytes Mitigates Alveolar Bone Loss in Mice With Periodontitis

et al. 2019

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Background: Periodontitis is a highly prevalent infection-triggered inflammatory disease that results in bone loss. Inflammation causes bone resorption by osteoclasts, and also by suppression of bone formation via increase of Dickkopf-1 (Dkk-1), an inhibitor of Wnt signaling. Here, we tested the hypothesis that osteocytic Dkk-1 is a key factor in the pathogenesis of periodontitis-induced alveolar bone loss (ABL). Methods: Twelve-week-old female mice with a constitutive deletion of Dkk-1 specifically in osteocytes (Dkk-1 fl/fl ;Dmp1:Cre) were subjected to experimental periodontitis (EP). Cre-negative littermates served as controls. EP was induced by placing a ligature around the upper 2nd left molar, the contralateral side was used as control. Mice were killed after 11 days and maxillae removed for micro-CT and histological analyses. The mRNA expression of Dkk-1, Runx2, Osteocalcin, OPG, RANKL, RANKL/OPG ratio, LEF-1, and TCF-7 were assessed in maxillae, while mRNA expressions of TNF and IL-1 were evaluated on gingiva using real-time PCR. Blood samples were collected for Dkk-1, CTX, and P1NP measurement by ELISA. Results: The deletion of Dkk-1 in osteocytes prevented ABL in mice with EP, compared to Cre-negative control mice with EP. Micro-CT analysis showed a significant reduction of bone loss (−28.5%) in EP Dkk-1 fl/fl ;Dmp1:Cre-positive mice compared to their littermate controls. These mice showed a greater alveolar bone volume, bone mineral density, trabecular number, and trabecular thickness after EP when compared to the Cre-negative controls. The local expression in maxillae as well as the serum levels of Dkk-1 were reduced in Dkk-1 fl/fl ;Dmp1:Cre-positive mice with EP. The transgenic mice submitted to EP showed increase of P1NP and reduction of CTX-I serum levels, and increase of TCF-7 expression. Histological analysis displayed less inflammatory infiltrates, a reduction of TNF and IL-1 expressions in the gingiva and fewer osteoclasts in Cre-positive animals with EP. Moreover, in mice with EP, the osteocytic deletion of Dkk-1 enhanced bone formation due to increased expressions of Runx2 and Osteocalcin and decreased expression of RANKL in maxillae. Conclusion: In summary, Dkk-1 derived from osteocytes plays a crucial role in ABL in periodontitis.

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Section: Introductionmentioning

confidence: 78%

Loss of Dkk-1 in Osteocytes Mitigates Alveolar Bone Loss in Mice With Periodontitis

et al. 2019

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“…Further, rat models have fewer limitations than in vitro models that cannot reproduce the complexity of interactions among the oral microbiome, environmental factors, and the immune/inflammatory host response (Struillou et al, 2010 ; Oz and Puleo, 2011 ; Hajishengallis et al, 2015 ). In this model, ligature causes mechanical trauma, and affects tissue integrity to induce an inflammatory response, increase oxidative stress, and destroy the periodontal ligament leading to significant bone loss (Bezerra et al, 2000 ; Guimarães et al, 2016 ; Lima et al, 2017 ; Oliveira et al, 2017 ). Alternative and preventive treatment options are essential to overcome the adverse effects of both antimicrobial and anti-inflammatory agents as an adjunct to conventional mechanical and surgical treatments (Chandra Shekar et al, 2015 ; Ramesh et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…Phytochemicals isolated from plants are considered good alternatives to synthetic chemicals. In recent years, the use of plant extracts has gained popularity (Alviano and Alviano, 2009 ; Chandra Shekar et al, 2015 ; Kala et al, 2015 ; Ramesh et al, 2016 ) and some of these extracts have been used to treat periodontitis or repair bone defects (Guimarães et al, 2016 ; Lima et al, 2017 ; Oliveira et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

“…Despite these data, there is no study in the literature focusing on inflammatory bone resorption using S. maritima L. extract or its bioactive phytochemicals. Thus, considering the involvement of both polymicrobial biofilm (Genco and Borgnakke, 2013 ; Hajishengallis, 2014 ) and oxidative stress (Tóthová et al, 2015 ; Lima et al, 2017 ) in periodontitis, and the potential therapeutic effect of S. maritima L., the goal of this study was to investigate the efficacy of S. maritima L. extract in a rat periodontitis model.…”

Section: Introductionmentioning

confidence: 99%

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Stemodia maritima L. Extract Decreases Inflammation, Oxidative Stress, and Alveolar Bone Loss in an Experimental Periodontitis Rat Model

et al. 2017

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Periodontitis is very prevalent worldwide and is one of the major causes of tooth loss in adults. About 80% of the worldwide population use medicinal plants for their health care. Stemodia maritima L. (S. maritima) antioxidant and antimicrobial effects in vitro as well as anti-inflammatory properties. Herein, the potential therapeutic effect of S. maritima was assessed in rats subjected to experimental periodontitis (EP). EP was induced in female Wistar rats by nylon thread ligature around 2nd upper left molars for 11 days. Animals received (per os) S. maritima (0.2; 1 or 5 mg/kg) or vehicle (saline + DMSO) 1 h before ligature and then once daily for 11 days. The naive group had no manipulation. After this time-point, the animals were terminally anesthetized, and the maxillae were removed for morphometric and histological analyzes (HE). Gingival tissues were dissected to cytokine levels detection (TNF-α, IL1-β, CINC-1, and IL-10), enzymes superoxide dismutase (SOD), and catalase (CAT) analysis, as well as gene expression (TNF-α, IL-1β, RANK, and iNOS) by qRT-PCR. Systemic parameters (weight variation, plasma levels of hepatic enzymes aspartate aminotransferase (AST) and alanine aminotransferase (ALT), creatinine, total alkaline phosphatase (TALP), and bone alkaline phosphatase (BALP) were performed. Histological analysis of the stomach, liver, kidney, and heart was also performed. S. maritima (5 mg/kg) decreased alveolar bone loss, TNF-α and CINC-1 gingival levels, oxidative stress, and transcription of TNF-α, IL1-β, RANK, and iNOS genes. It elevated both BALP activity and IL-10 gingival levels. The animals showed no any signs of toxicity. In conclusion, S. maritima reduced pro-inflammatory cytokine production, oxidative stress, and alveolar bone loss in a pre-clinical trial of periodontitis. S. maritima is a potential tool for controlling the development of periodontitis.

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“…This process is able to stimulate the inflammatory response that causes the destruction of the periodontal tissues 11,12 . In addition to the antimicrobial effect 13 , another relevant point is the effect of stimulating the immune system through glutamine.…”

Section: Discussionmentioning

confidence: 99%

Effect of glutamine ingestion on the progression of induced periodontitis: experimental study in rats

Silva

Segundo

Semenoff

et al. 2018

Rev. odontol. UNESP

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ResumoIntrodução: Com a suplementação de glutamina há um melhor desempenho no sistema de defesa do corpo. Objetivo: O objetivo deste estudo foi analisar o efeito da suplementação de glutamina na doença periodontal induzida por ligatura em ratos. Material e método: Foram selecionados 48 ratos machos da linhagem Wistar divididos em 4 grupos (N = 12): grupo controle (GC) incluindo animais saudaveis recebendo solução salina diariamente via gavagem; grupo glutamina (GG) incluindo animais saudaveis que receberam suplementação oral de glutamina por gavagem a uma dose de 1,5 g/kg/dia; grupo periodontite (GP) incluindo animais com doença periodontal induzida na maxila em ambos os lados recebendo solução salina diária via gavagem; grupo experimental (GE) incluindo animais com doença periodontal induzida na maxila nos dois lados recebendo glutamina diária via gavagem na dose de 1,5 g/kg/dia. No 30º dia, todos os animais foram eutanasiados por excesso de anestésico. Descritores: Suplementos nutricionais; doenças periodontais; produtos com ação antimicrobiana. AbstractIntroduction: With glutamine supplementation there is better performance in the body's defense system. Objective: The aim of this study was to analyze the effect of glutamine supplementation on ligand-induced periodontal disease in rats. Material and method: 48 selected male Wistar rats were divided into 4 groups (N = 12): control group (CG) including healthy animals receiving daily saline solution via gavage; glutamine group (GG) including healthy animals receiving oral glutamine supplementation by gavage at a dose of 1.5 g / kg / day; periodontitis group (PG) including animals with induced periodontal disease on both upper sides receiving daily saline solution via gavage; experimental group (EG) including animals with induced periodontal disease on both upper sides receiving daily glutamine via gavage at a dose of 1.5 g / kg / day. On the 30th day, all animals were euthanized by excess anesthetic.

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The Effect of Calendula officinalis on Oxidative Stress and Bone Loss in Experimental Periodontitis

Cited by 33 publications

References 54 publications

Loss of Dkk-1 in Osteocytes Mitigates Alveolar Bone Loss in Mice With Periodontitis

Loss of Dkk-1 in Osteocytes Mitigates Alveolar Bone Loss in Mice With Periodontitis

Stemodia maritima L. Extract Decreases Inflammation, Oxidative Stress, and Alveolar Bone Loss in an Experimental Periodontitis Rat Model

Effect of glutamine ingestion on the progression of induced periodontitis: experimental study in rats

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