Effects of bone morphogenetic protein‐4 (<scp>BMP</scp>‐4) on adipocyte differentiation from mouse adipose‐derived stem cells

Wei, Xiaoyuan; Li, G.; Yang, Xueting; Ba, Kai; Fu, Yang; Fu, Na; Cai, Xiaoxiao; Chen, Q.; Wang, M.; Lin, Yunfeng

doi:10.1111/cpr.12044

Cited by 14 publications

(12 citation statements)

References 42 publications

(55 reference statements)

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“…It is well known that BMP-7 is an important inducer of adipocyte differentiation [35]. In the present study, both PDL cells and DF progenitor cells showed no signs of adipocyte markers, such as LPL and PPARG.…”

Section: Gene Expressions During Differentiation Inductionsupporting

confidence: 41%

Isolation, characterization and investigation of differentiation potential of human periodontal ligament cells and dental follicle progenitor cells and their response to BMP-7 in vitro

Açil

Yang

Gülses³

et al. 2015

Odontology

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The aim of this study was to assess the factors, mechanisms and the differences between periodontal ligament (PDL) cells and denta l follicle (DF) progenitor cells towards the osteoblastic/cementoblastic differentiation and to investigate the effects of BMP-7 on developmental (DF) and mature tissue-derived (PDL) cells, respectively. Primary cell culture of PDL cells and DF progenitor cells was performed. Osteogenic differentiation was evaluated using von Kossa, Alizarin Red S and immuno-histo-chemistry staining of osteocalcin. Gene expression pattern was evaluated via real-time PCR. A series of CD surface marks were tested using flow cytometry and fluorescence-activated cell-sorting analysis was performed. Real-time RT-PCR demonstrated similar gene expression pattern of PDL cells and DF progenitor cells: the expression of OPN and OCN significantly was elevated when incubated with osteogenic components, Runx2 was unaffected, and Osteorix was hardly expressed whether in basic medium or induction medium. In addition, BMP-7 induced osteoblast/cementoblast differentiation of PDLSCs and DF progenitor cells in a dose- and time-dependent manner, as reflected by enhanced Runx2 and (OCN) mRNA transcript expression. BMP-7 triggers PDL cells and DF progenitor cells to differentiate towards an osteoblast/cementoblast phenotype.

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Section: Gene Expressions During Differentiation Inductionsupporting

confidence: 41%

Isolation, characterization and investigation of differentiation potential of human periodontal ligament cells and dental follicle progenitor cells and their response to BMP-7 in vitro

Açil

Yang

Gülses³

et al. 2015

Odontology

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“…BMPs can trigger MSCs to activate transcription factors that are specific to their tissue origin in vivo, such as Pparg and Runx2, in adipogenic and osteogenic specification, respectively (Beederman et al, 2013;Wei et al, 2013). Similarly, BMP signaling activates dental pulp MSCs to express odontoblast markers (Iohara et al, 2004;Casagrande et al, 2010).…”

Section: A Bmp-regulated Transcription Factor Network Controls Odontomentioning

confidence: 99%

“…In adult organs, BMP signaling helps maintain the stem cell population size by inhibiting stem cell proliferation and niche expansion (He et al, 2004). BMP signaling activity is also required for activating MSCs to undergo differentiation in cell culture (Beederman et al, 2013;Wei et al, 2013). However, it is not clear how BMP signaling controls MSC cell fate determination during tooth root development.…”

Section: Introductionmentioning

confidence: 99%

BMP signaling orchestrates a transcriptional network to control the fate of mesenchymal stem cells in mice

Feng

Jing

et al. 2017

Development

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Signaling pathways are used reiteratively in different developmental processes yet produce distinct cell fates through specific downstream transcription factors. In this study, we used tooth root development as a model with which to investigate how the BMP signaling pathway regulates transcriptional complexes to direct the fate determination of multipotent mesenchymal stem cells (MSCs). We first identified the MSC population supporting mouse molar root growth as Gli1 + cells. Using a Gli1-driven Cre-mediated recombination system, our results provide the first in vivo evidence that BMP signaling activity is required for the odontogenic differentiation of MSCs. Specifically, we identified the transcription factors Pax9, Klf4, Satb2 and Lhx8 as being downstream of BMP signaling and expressed in a spatially restricted pattern that is potentially involved in determining distinct cellular identities within the dental mesenchyme. Finally, we found that overactivation of one key transcription factor, Klf4, which is associated with the odontogenic region, promotes odontogenic differentiation of MSCs. Collectively, our results demonstrate the functional significance of BMP signaling in regulating MSC fate during root development and shed light on how BMP signaling can achieve functional specificity in regulating diverse organ development.

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“…13 Also, Lilja et al found that bone marrow-derived precursor cells infiltrate into adipose tissue under inflammation and become mature adipocytes; this further suggests that most of the regeneration cells that take part in adipogenesis come from recipient circulation. 20 Adipose-derived stromal cells have adipogenic ability, [21][22][23][24] and CD34 + adipose precursor cells may play an important role during adipose tissue regeneration. 25 In the threezone theory reported by Yoshimura et al, they point out that the survival of fat graft was largely determined by adipose tissue regeneration after transplantation and the CD34 + cells were very likely to be the seed cells of the adipogenic progress.…”

Section: Figmentioning

confidence: 99%

The Angiogenic and Adipogenic Modes of Adipose Tissue after Free Fat Grafting

Dong

Peng

Chang

et al. 2015

Plastic and Reconstructive Surgery

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Effects of bone morphogenetic protein‐4 (BMP‐4) on adipocyte differentiation from mouse adipose‐derived stem cells

Abstract: Our present study suggests that BMP-4, as an adipo-inducing factor, promoted adipogenesis of ASCs at higher concentrations (50 ng/ml) and can perhaps be considered as a candidate for use in adipose tissue engineering.

Cited by 14 publications

References 42 publications

Isolation, characterization and investigation of differentiation potential of human periodontal ligament cells and dental follicle progenitor cells and their response to BMP-7 in vitro

Isolation, characterization and investigation of differentiation potential of human periodontal ligament cells and dental follicle progenitor cells and their response to BMP-7 in vitro

BMP signaling orchestrates a transcriptional network to control the fate of mesenchymal stem cells in mice

The Angiogenic and Adipogenic Modes of Adipose Tissue after Free Fat Grafting

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