Effects of Bone Marrow Mesenchymal Stem Cell to Transforming Grow Factor-β3 and Matrix Metalloproteinase-9 Expression in Burns

Revilla, Gusti; Afriani, Nita; Rusnita, Dewi

doi:10.3923/jms.2018.164.171

Cited by 4 publications

(3 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This pressure equals the mass of 51 g of aluminum bar [ 24 ]. For group III (42 rats for burned animals treated with BM-MSCs), the isolated BM-MSCs were suspended in an incomplete DMEM with viability greater than 95% and rapidly injected subcutaneously in the burned animals at a dose of 2 × 10 6 cells/ml [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Therapeutic effect of mesenchymal stem cells on histopathological, immunohistochemical, and molecular analysis in second-grade burn model

et al. 2021

View full text Add to dashboard Cite

Background and aim Deleterious cutaneous tissue damages could result from exposure to thermal trauma, which could be ameliorated structurally and functionally through therapy via the most multipotent progenitor bone marrow mesenchymal stem cells (BM-MSCs). This study aimed to induce burns and examine the effect of BM-MSCs during a short and long period of therapy. Material and methods Ninety albino rats were divided into three groups: group I (control); group II (burn model), the animals were exposed to the preheated aluminum bar at 100°C for 15 s; and group III (the burned animals subcutaneously injected with BM-MSCs (2×106 cells/ ml)); they were clinically observed and sacrificed at different short and long time intervals, and skin samples were collected for histopathological and immunohistochemical examination and analysis of different wound healing mediators via quantitative polymerase chain reaction (qPCR). Results Subcutaneous injection of BM-MSCs resulted in the decrease of the wound contraction rate; the wound having a pinpoint appearance and regular arrangement of the epidermal layer with thin stratum corneum; decrease in the area percentages of ADAMs10 expression; significant downregulation of transforming growth factor-β (TGF-β), interleukin-6 (IL-6), tumor necrotic factor-α (TNF-α), metalloproteinase-9 (MMP-9), and microRNA-21; and marked upregulation of heat shock protein-90α (HSP-90α) especially in late stages. Conclusion BM-MSCs exhibited a powerful healing property through regulating the mediators of wound healing and restoring the normal skin structures, reducing the scar formation and the wound size.

show abstract

Section: Methodsmentioning

confidence: 99%

Therapeutic effect of mesenchymal stem cells on histopathological, immunohistochemical, and molecular analysis in second-grade burn model

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The immune system is stimulated by mesenchymal stem cells MSCs to release cytokines and chemokines. (4) BMSCs enhanced the wound healing and skin regeneration by supplying new cells as fibroblasts and keratinocytes (5) .…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study Between the Therapeutic Role of Bone Marrow-Derived Mesenchymal Stem Cells Versus Platelet Rich Plasma in Skin Burn Healing in Albino Rats

2023

The Egyptian Journal of Hospital Medicine

View full text Add to dashboard Cite

Background and Objective: Mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) accelerate wound healing. This study aimed to comparing the therapeutic role of BMSCs & PRP in skin burn healing. Material and Methods:The study included forty albino rats, five rats were used to supply PRP and five rats were considered as a control group. The other thirty rats were exposed to thermal injury to induce 2 nd degree skin burn then divided into three groups of ten rats each; the skin burned group including rats that received no further treatment, BMSCs-treated group and PRP-treated group. Each group was subdivided into two equal subgroups and examined on days 7 & 21 after the burn. Skin specimens of all groups were handled, processed to be stained with H&E, Masson's trichrome for histological examination and by VEGF, CD105 for immunohistochemical examination. Results: Injection the burned skin with BMSCs or PRP caused re-epithelization of the epidermis, reduction of inflammation, increased collagen fibers deposition and acceleration of angiogenesis. Visible epidermis and dermis regeneration were more evident compared to the burned group. However, the improvement of histological findings was remarkable in the BMSCs-treated group than in PRP treated one. Moreover, the main area percentage of collagen fibers deposition and VEGF immunostaining were significantly increased in the BMSCs treated group in comparison to PRP treated group. Conclusion: BMSCs displayed better healing effect than PRP as evidenced by collagen fibers deposition and angiogenesis and returning the normal histological structure of the skin.

show abstract

“…[7] MSCs improved the process of burn healing, reduced the formation of scars and re-established the normal function of the skin and its appendages [8] . In skin wound healing, angiogenesis is necessary for formation of new granulation tissue in the proliferative phase [9] .…”

Section: Introductionmentioning

confidence: 99%

Histological and Immunohistochemical Studies on the Role of Stem Cells on the Burned Skin of Adult Male Albino Rats

Allah

Moustafa

Moahmmed

et al. 2020

International Journal of Medical Arts

View full text Add to dashboard Cite

Background: Mesenchymal stem cells have delivered new approaches to the management of burn healing in severe skin injuries. Aim of the work: To evaluate the effect of bone marrow-derived mesenchymal stem cells [BMSCs] on healing of induced deep 2nd degree skin burns in albino rats. Methodology: 55 male albino rats weighed 200gm were divided into 5 groups, 5 animals in group I [control] and group II [burn model], 15 animals in group III [untreated], group IV and group V [MSCs treated]. Groups III, IV and V were subdivided into 3 subgroups that were sacrificed after 7, 14 and 21 days respectively. Deep 2nd degree burn wounds were induced on a 4 cm2 area on the back of rats in groups II, III, IV and V by brass probes stabilized at 88-90°C and contacted to the shaved dorsal skin of rats for 20 seconds without exerting any external pressure. Mesenchymal stem cells were isolated from the bone marrow of 5 young rats, weighed 100gm then intra-dermally and intraperitoneally injected. Results: By the end of the third week, the wounds of BMSCs-treated groups showed full regeneration of epidermis, reorganization of collagen and decrease in VEGF immunopositive cells. Delayed wound healing was seen in 20% of systemically treated rats. Significant increase in the mean area percent of collagen fibers was detected in topically treated group. Conclusion: Both methods of BMSCs injection were effective in healing of full thickness skin wound but topical method was more effective.

show abstract

Effects of Bone Marrow Mesenchymal Stem Cell to Transforming Grow Factor-β3 and Matrix Metalloproteinase-9 Expression in Burns

Cited by 4 publications

References 23 publications

Therapeutic effect of mesenchymal stem cells on histopathological, immunohistochemical, and molecular analysis in second-grade burn model

Therapeutic effect of mesenchymal stem cells on histopathological, immunohistochemical, and molecular analysis in second-grade burn model

A Comparative Study Between the Therapeutic Role of Bone Marrow-Derived Mesenchymal Stem Cells Versus Platelet Rich Plasma in Skin Burn Healing in Albino Rats

Histological and Immunohistochemical Studies on the Role of Stem Cells on the Burned Skin of Adult Male Albino Rats

Contact Info

Product

Resources

About