Effects of BMP-2, BMP-4, and BMP-6 on Osteoblastic Differentiation of Bone Marrow-Derived Stromal Cell Lines, ST2 and MC3T3-G2/PA6

Yamaguchi, Akira; Ishizuya, Toshinori; Kintou, Naoki; Wada, Yasuhiro; Katagiri, Takenobu; Wozney, John M.; Rosen, Vicki; Yoshiki, Shusaku

doi:10.1006/bbrc.1996.0411

Cited by 318 publications

(193 citation statements)

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“…The expression of BMP-2 was also evaluated as an indicator of osteoblast activity because it induces osteoblast differentiation [42]. The results exhibit remarkably higher osteoblast activation in the presence of TESPSA, since the gene expression level of BMP-2 increased more than a hundredfold at all measured times (Figure 3c).…”

Section: Discussionmentioning

confidence: 96%

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Godoy-Gallardo

Guillem-Marti

Sevilla

et al. 2016

Materials Science and Engineering: C

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Bacterial infection in dental implants along with osseointegration failure usually leads to loss of the device. Bioactive molecules with antibacterial properties can be attached to titanium surfaces with anchoring molecules such as silanes, preventing biofilm formation and improving osseointegration. Properties of silanes as molecular binders have been thoroughly studied, but research on the biological effects of these coatings is scarce. The aim of the present study was to determine the in vitro cell response and antibacterial effects of triethoxysilypropyl succinic anhydride (TESPSA) silane anchored on titanium surfaces. X-ray photoelectron spectroscopy confirmed a successful silanization. The silanized surfaces showed no cytotoxic effects. Gene expression analyses of Sarcoma Osteogenic (SaOS-2) osteoblast-like cells cultured on TESPSA silanized surfaces reported a remarkable increase of biochemical markers related to induction of osteoblastic cell differentiation. A manifest decrease of bacterial adhesion and biofilm formation at early stages was observed on treated substrates, while favoring cell adhesion and spreading in bacteria-cell co-cultures.Surfaces treated with TESPSA could enhance a biological sealing on implant surfaces against bacteria colonization of underlying tissues. Furthermore, it can be an effective anchoring platform of biomolecules on titanium surfaces with improved osteoblastic differentiation and antibacterial properties.

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Section: Discussionmentioning

confidence: 96%

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Godoy-Gallardo

Guillem-Marti

Sevilla

et al. 2016

Materials Science and Engineering: C

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“…In the present study, we used the stromal cell line ST2, derived from mouse bone marrow, to examine CXCL12 expression. In normal culture, ST2 cells have the characteristics of preadipocytes and none of the features typical of the osteoblastic phenotype (Yamaguchi et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Regulation of CXCL12 expression by canonical Wnt signaling in bone marrow stromal cells

Tamura

Sato

Nashimoto

2011

The International Journal of Biochemistry & Cell Biology

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“…This indicates that the different BMPs have different signal transduction pathways. 27,45,58,60,61 The different BMP receptors and signaling pathways may be the critical factors that account for the different osteogenic activity of BMP viral vectors in Sprague-Dawley rats. Some BMP (eg BMP-2 and BMP-4) signaling pathways may be completely blocked by the immune response stimulated by the adenovirus.…”

Section: Discussionmentioning

confidence: 99%

“…24 The effects of BMP-2, BMP-4, and BMP-6 were also compared in fetal rat secondary calvarial cell culture system, rat osteoprogenitor cells, and bone marrow stromal cells. [25][26][27] The relative activity of different BMPs varied among the cell types exposed to these proteins.…”

Section: Introductionmentioning

confidence: 99%

Osteogenic potential of five different recombinant human bone morphogenetic protein adenoviral vectors in the rat

et al. 2003

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one morphogenetic protein (BMP) adenoviral vectors for the induction of osteogenesis are being developed for the treatment of bone pathology. However, it is still unknown which BMP adenoviral vector has the highest potential to stimulate bone formation in vivo. In this study, the osteogenic activities of recombinant human BMP-2, BMP-4, BMP-6, BMP-7, and BMP-9 adenoviruses were compared in vitro, in athymic nude rats, and in Sprague-Dawley rats. In vitro osteogenic activity was assessed by measuring the alkaline phosphatase activity in C2C12 cells transduced by the various BMP vectors. The alkaline phosphatase activity induced by 2 Â 10 5 PFU/well of BMP viral vector was 4890 Â 10 À12 U/well for ADCMVBMP-9, 302 Â 10 À12 U/well for ADCMVBMP-4, 220 Â 10 À12 U/well for ADCMVBMP-6, 45 Â 10 À12 U/well for ADCMVBMP-2, and 0.43 Â 10 À12 U/ well for ADCMVBMP-7. The average volume of new bone induced by 10 7 PFU of BMP vector in athymic nude rats was 0.3770.03 cm 3 for ADCMVBMP-2, 0.8970.07 cm 3 for ADCMVBMP-4, 1.0270.07 cm 3 for ADCMVBMP-6, 0.2470.05 cm 3 for ADCMVBMP-7, and 0.6370.07 cm 3 for ADCMVBMP-9. In immunocompetent Sprague-Dawley rats, no bone formation was demonstrated in the ADCMVBMP-2, ADCMVBMP-4, and ADCMVBMP-7 groups. ADCMVBMP-6 at a viral dose of 10 8 PFU induced 0.1070.03 cm 3 of new bone, whereas ADCMVBMP-9 at a lower viral dose of 10 7 PFU induced more bone, with an average volume of 0.2970.01 cm 3 .

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Effects of BMP-2, BMP-4, and BMP-6 on Osteoblastic Differentiation of Bone Marrow-Derived Stromal Cell Lines, ST2 and MC3T3-G2/PA6

Cited by 318 publications

References 0 publications

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Regulation of CXCL12 expression by canonical Wnt signaling in bone marrow stromal cells

Osteogenic potential of five different recombinant human bone morphogenetic protein adenoviral vectors in the rat

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