Effects of Astragalus Polysaccharides Nanoparticles on Cerebral Thrombosis in SD Rats

Abstract

ObjectiveTo investigate the efficacy and improvement of Astragalus polysaccharides (APS) and APS-nano on cerebral thrombosis in rats.MethodsA total of 72 SD rats were randomly divided into NC group, Model group, APS-Nano group, and APS group. The cerebral thrombosis Model of SD rats was established by injecting compound thrombus inducer into the internal carotid artery. After 14 days of different intervention treatments, the TTC staining of brain tissue were performed, and A/left brain wet weight ratio, left brain/right brain wet weight ratio, blood rheology indexes, and coagulation function indexes of cerebral thrombosis were measured. ELISA was used to measure the contents of thromboxane 2 (TXB2), 6-keto-prostaglandin F1α (6-Keto-PGF1α), tissue factor (TF), neuron-specific enolase (NSE), S-100β, catenin (CAT), superoxide dismutase (SOD), as well as malondialdehyde (MDA). The binding specificity between miR-885-3p and TF was verified by the double-luciferin reporting experiment, and western blot was used to measure the expression level of TF protein.ResultsCompared with the Model group, after treatment with APS-nano or APS, the ratio of left brain/right brain wet weight decreased significantly. Whole blood low shear viscosity (WBLSV), whole blood high shear viscosity (WBHSV), plasma viscosity (PV), and erythrocyte aggregation index (Arbc) was all reduced. In addition, prothrombin time (PT) and activated partial thromboplastin time (APTT) were increased, and fibrinogen (FIB) content was decreased. The expression of TXB2, 6-Keto-PGF1α, and TF showed a downward trend. Similarly, the expression of TF protein was decreased. Furthermore, the contents of NSE and S-100β proteins were all decreased, whereas the contents of CAT and SOD were increased, and the contents of MDA was decreased. At the same dose, compared with APS treatment, APS-nano treatment had a significant inhibitory effect on cerebral thrombosis in rats. Finally, we found that TF is a target gene of miR-885-3p and specifically binds to miR-885-3p.ConclusionAPS has a significant inhibitory effect on the formation of cerebral thrombosis induced by compound thrombus inducers. Moreover, APS-nano has a more significant inhibitory effect on cerebral thrombosis. Meanwhile, the regulation of miR-885-3p regulating TF expression may be related to the occurrence of cerebral thrombosis.

Astragalus membranaceus Additive Improves Serum Biochemical Parameters and Reproductive Performance in Postpartum Dairy Cows

“…In other words, the AM used in this study did not induce thrombus while promoting blood coagulation in cows. The result of a previous study involving rat-fed AM-containing feed is consistent with the conclusions of this study ( 31 ). This is also in line with the theory of qi and blood in traditional Chinese medicine ( 32 ).…”

A Review of Traditional Chinese Medicine, Buyang Huanwu Decoction for the Treatment of Cerebral Small Vessel Disease

“…Modern pharmacological studies suggest that the active ingredients of Astragalus include astragalus polysaccharide, astragalus saponins, flavonoids calycosin, 3-hydroxy-9,10-dimethoxy pterostane, and astragaloside I, III, V. Astragalus polysaccharide is the main pharmacologically active ingredient in Astragalus . Studies have reported that it can suppress mitochondrial damage-mediated apoptosis ( Gao et al, 2021b ), modulate gut microbiota ( Zhuang et al, 2021 ), enhance immune response ( Zhou et al, 2021 ), alleviate cognitive impairment and β-amyloid accumulation via Nrf2 pathway ( Qin et al, 2020 ), enhance remyelination ( Ye et al, 2021 ), inhibit the formation of cerebral thrombosis ( Sun et al, 2020 ), protect neurons, and stabilize mitochondrial in a Parkinson disease (PD) mouse model ( Liu et al, 2018 ), and thus exhibit mitochondrial and anti-aging activity ( Li et al, 2012 ).…”

Herb Polysaccharide-Based Drug Delivery System: Fabrication, Properties, and Applications for Immunotherapy

“…The combination of potential pharmacological activity and useful (bio)physical properties increases the value of HPS as building blocks for DDS, which are active treatment agents. Previous studies have reported the synthesis of HPS-DDS, such as nanoparticles (NPs) [25,26], micelles [27], liposomes [28], microemulsions [29], hydrogels [30], and microneedles [31], for the treatment of various diseases. These studies show that HPS can serve as drug carriers [32], stabilizers [33], solubilizers [34], and emulsifiers [35].…”