Effects of Aqueous Extract of <em>Cyphostemma glaucophilla</em> Leaves on Some Specific and Non-specific Immune Responses in Albino Rats

Abstract

Cyphostemma glaucophilla is used in the management of kwashiorkor, because impaired immune responses is associated with malnourishment the modulatory activity of aqueous leaves extract of Cyphostemma glaucophilla on primary and secondary humuoral responses, in vivo leucocyte mobilization, Delayed Type Hypersensitive Reaction (DTHR), haemoglobin, packed cell volume and white blood cell count were evaluated. The extract at 250 and 500 mg/kg stimulated significant (p<0.05) dose dependent increase in primary and secondary sheep red blood cell specific antibody titre comparable to Levamisol. Extract at doses of 100 and 250 mg/kg induced leucocyte mobilization while 500 mg/kg inhibited leucocyte mobilization. The extract at 250 and 500 mg/kg produced significant (p<0.05) inhibition of DTHR in rats by 20.24 and 67.77%, respectively. The extract also induced significant (p<0.05) increases in packed cell volume by 3.0% at the lowest dose of 0.5 mg/kg and 11.2% at the highest dose of 2.0 mg/kg. However, the extract was insensitive to haemoglobin and total white blood cell count. Result has established cellular and humoral immunomodulatory activities of Cyphostemma glaucophilla aqueous leaves extract.

In-vivo evaluation of immunomodulatory activity of crude extract and solvent fractions of Cyphostemma adenocaule (Steud. ex A.Rich)

“…This finding is consistent with the finding of Feyisayo and Oluokun which showed the antibody precipitating capacity of a polysaccharide fraction of C. adenocaule in the agar well diffusion method [ 37 ]. It is also in line with the study of Ojogbane and his coworkers that reported stimulation of HAT by aqueous extract of C. glaucophilla leaves [ 38 ].…”