In-vivo evaluation of immunomodulatory activity of crude extract and solvent fractions of Cyphostemma adenocaule (Steud. ex A.Rich)

Abebe, Dehnnet; Karim, Aman; Bitew, Helen; Periasamy, Gomathi

doi:10.1016/j.heliyon.2022.e12377

Cited by 2 publications

(2 citation statements)

References 28 publications

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“… 23 The stimulation of the hematological system and lymphocyte differentiation may be associated with the increased carbon clearance rate. 38 Both essential oils showed a dose-dependent effect on mice’s ability to remove carbon particles. The 400 mg/kg had the greatest impact on the outcome.…”

Section: Discussionmentioning

confidence: 98%

In-vivo Immunomodulatory Activities of Essential Oils of Artemisia abyssinica and Lepidium sativum in Mice

Ayenew,

Ayalew,

Wondmkun

et al. 2024

ITT

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Background Ethiopians use Artemisia abyssinica and Lepidium sativum as immunity enhancers. However, there is no scientific validation conducted so far regarding this claim. The aim of this study was to investigate the in-vivo immunomodulatory activities of essential oils of A. abyssinica and L. sativum in mice. Methods The extraction was carried out using the earlier techniques. By hydro distilling fresh seeds and aerial portions of A. abyssinica and L. sativum , respectively, essential oils were obtained. Essential oils of both plants were tested at 100, 200 and 400 mg/kg. The rate of carbon clearance, humoral antibody titer, delayed type hypersensitivity response, spleen and thymus indices were evaluated in mice according to scientific protocols. The carbon clearance assay was determined using carbon ink. Sheep red blood cell was used as an antigen for other tests. Results Essential oils of A. abyssinica and L. sativum at 400 mg/kg significantly increased the rate of carbon clearance from the body of mice ( p <0.05). The maximum carbon clearance rate was achieved for A. byssinica essential oil at 400 mg/kg. Both essential oils raised the level of HAT to SRBC in comparison to the vehicle and cyclophosphamide administered groups. The largest (84.668±1.951) mean secondary HAT to SRBC was generated by L. sativum essential oil at 400 mg/kg ( p <0.001). A. abyssinica essential oil at 200 and 400 mg/kg significantly increased the level of thymus index compared to the model group ( p <0.05 and 0.01 respectively). The levamisole group experienced the highest increase in thymus index ( p <0.001). Essential oil of L. sativum at 400 mg/kg also increased the level of thymus index. The spleen index in mice was improved by the essential oils only at the highest dose levels (400 mg/kg). Conclusion It can be inferred that the essential oils of L. sativum and A. abyssinica have immunostimulant properties.

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Section: Discussionmentioning

confidence: 98%

In-vivo Immunomodulatory Activities of Essential Oils of Artemisia abyssinica and Lepidium sativum in Mice

Ayenew,

Ayalew,

Wondmkun

et al. 2024

ITT

View full text Add to dashboard Cite

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“…Centrifuging the blood at 2500 rpm for 10 minutes allowed the SRBCs to be separated, and the collected blood was then rinsed with pyrogen-free normal saline (0.9% w/v). After that, the concentration was adjusted to 1 × 10 8 SRBC in 1 mL of sheep blood [18].…”

Section: Sheep Red Blood Cell (Srbc) Preparation and Standardizationmentioning

confidence: 99%

In vitro Antioxidant, In vivo Bioavailability, and Immunomodulatory Effects of a Polyherbal Formulation (Storg B) Induced by Cyclophosphamide in an Experimental Animal Model

Firoz H M,

Sadashiva C T,

Sahana C V

2024

J. Pharm. Res. Int.

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Introduction: The immunomodulatory properties of plants are being extensively explored these days as they contain a wide range of bioactive compounds that can treat immunomodulatory disorders. The aim of the present investigation was to determine the efficacy of the standardized polyherbal formulation (Storg-B). The Storg-B combines Psidium guajava, Mangifera indica, and Citrus limon. Methods: The aqueous extract of the Storg B was investigated for DPPH radical scavenging activity to determine antioxidant activity. SD rats were selected for the evaluation of the immunomodulatory activity of Storg-B. The haemagglutination and hematology methods were used to evaluate the immunomodulatory studies. For the pharmacokinetic studies, the LC-MS/MS technique is employed to quantify Storg B. Results: The percentage inhibition of DPPH radial IC50 values of standard BHT and Storg-B were 60.52 and 68.35µg/ml, respectively. In the in vivo animal studies, Storg-B showed significant immunomodulatory activity by increasing the primary and secondary antibody titer values by 6.58 and 8.25 mg/mL, respectively, for the SRBC antigen at a dose of 100 mg/kg. There’s also an increase in the neutrophil adhesion percentage at the testing doses. Results of hematological parameters revealed that Storg-B restored all the levels of blood components at a 100 mg/kg dose. A Storg-B showed greater potency in the bioavailability studies. Conclusion: Thus, the Storg-B used as an efficient and secure formulation in the treatment of immune deficiency by improving the immune response.

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In-vivo evaluation of immunomodulatory activity of crude extract and solvent fractions of Cyphostemma adenocaule (Steud. ex A.Rich)

Cited by 2 publications

References 28 publications

In-vivo Immunomodulatory Activities of Essential Oils of Artemisia abyssinica and Lepidium sativum in Mice

In-vivo Immunomodulatory Activities of Essential Oils of Artemisia abyssinica and Lepidium sativum in Mice

In vitro Antioxidant, In vivo Bioavailability, and Immunomodulatory Effects of a Polyherbal Formulation (Storg B) Induced by Cyclophosphamide in an Experimental Animal Model

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