Effects of amniotic membrane extract on primary human corneal epithelial and limbal cells

Dudok, David; Nagdee, Imraan; Cheung, Kevin; Liu, Hong; Vedovelli, Luca; Ghinelli, Emiliano; Kenyon, Kenneth R.; Parapuram, Sunil K.; Hutnik, Cindy

doi:10.1111/ceo.12480

Cited by 35 publications

(33 citation statements)

References 18 publications

(35 reference statements)

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“…Liang et al demonstrated the benefits of AME for ocular chemical burns with a preliminary study demonstrating its ability to reduce corneal inflammation and promote re-epithelization (65) . Dudok et al used AME to treat corneal and limbal epithelial cells under mechanical stress, which healed faster (66) . Collectively, these results suggests the potential benefit of AME for the treatment of acute corneal injuries.…”

Section: Therapeutic Use Of Gf/cytokines In Ocular Surface Diseasesmentioning

confidence: 99%

Biological modulation of corneal epithelial wound healing

Loureiro

Gomes

2019

Arquivos Brasileiros De Oftalmologia

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The transparency and maintenance of corneal epithelial integrity are essential for its optical properties and, to preserve these characteristics, the epithelium undergoes continuous renewal. This renewal depends on the control of cell proliferation and differentiation mediated by mitogenic factors responsible for increasing mitoses and stimulating cellular migration. Cell-cell communication plays a pivotal role in epithelial healing process, and several cytokines and growth factors are involved in this process. Understanding the cross-talk and paracrine effects of these cytokines and growth factors released can help in the search for new therapeutic strategies to treat ocular surface diseases.

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Section: Therapeutic Use Of Gf/cytokines In Ocular Surface Diseasesmentioning

confidence: 99%

Biological modulation of corneal epithelial wound healing

Loureiro

Gomes

2019

Arquivos Brasileiros De Oftalmologia

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“…Most of the beneficial effects of AM are attributed to biomolecules, such as fibronectin, hepatocyte growth factor (HGF), epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), transforming growth factor (TGF), and collagen types I, III, IV, and V, which are potent sources for corneal regeneration. 18 They combine to facilitate migration, adhesion, and differentiation of the corneal epithelium, while preventing apoptosis. AM downregulates the expression of such cell surface markers as CD80, CD86, and major histocompatibility complex class 2 antigen, which are modulators of the immune response.…”

Section: Introductionmentioning

confidence: 99%

“…AME contains many of the similar properties of cryopreserved AM. 18 Similar to AM, it has high levels of a myriad of growth factors, including EGF, HGF, bFGF, protease inhibitors, and a novel matrix component known as heavy chain-hyaluronan pentraxin 3 (HC-HA/PTX3), which acts to prevent inflammation, angiogenesis, and scarring. 17 , 22 AME retains anti-inflammatory activities and often acts through downregulating and inducing apoptosis in macrophages.…”

Section: Introductionmentioning

confidence: 99%

Amniotic membrane extract and eye drops: a review of literature and clinical application

Murri¹,

Moshirfar²,

Birdsong³

et al. 2018

OPTH

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The amniotic membrane (AM) has a long history of use in the treatment of various diseases of the ocular surface. It contains pluripotent cells, highly organized collagen, anti-fibrotic and anti-inflammatory cytokines, immune-modulators, growth factors, and matrix proteins. It is used to promote corneal healing in severely damaged eyes. Recently, AM extract and AM extract eye drops have been successfully used in clinical applications, including dry eye and chemical burns. We provide an overview on the recent progress in the preparation, mechanisms of action, and use of AM extract/AM extract eye drops for corneal and external eye diseases.

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“…The article presented in this issue by Dudok et al . provides insights into the mode of action of AMX.…”

mentioning

confidence: 99%

“…This humoral approach is exemplified by the use of amniotic membrane extract (AMX), as pioneered by Tseng's group, 18,19 and demonstrated to be effective both in the laboratory 20,21 and in clinical trials. 22,23 The article presented in this issue by Dudok et al 2 provides insights into the mode of action of AMX. The authors used in vitro wounding models of corneal epithelial cell lines in the laboratory.…”

mentioning

confidence: 99%