Effects of Adipose-derived Stromal Cells and of their Extract on Wound Healing in a Mouse Model

Lim, Jin Soo; Yoo, Gyeol

doi:10.3346/jkms.2010.25.5.746

Cited by 51 publications

(38 citation statements)

References 14 publications

(25 reference statements)

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“…Many types of cytokines and growth factors are responsible for inflammation, reepithelialization, the formation of granulation tissue, and neovascularization during the wound healing process [23]. Transforming growth factor beta is an important growth factor that regulates different cellular functions in all phases of wound healing.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Wound Healing Potential ofResina Draconis (Dracaena cochinchinensis)in Animal Models

Liu

Lin

Xiao

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Resina Draconis (RD) is a type of dragon's blood resin obtained from Dracaena cochinchinensis (Lour.) S.C. Chen (Yunnan, China). It has been used as a medicine since ancient times by many cultures. The ethanolic extract of Resina Draconis (RDEE) was evaluated for its wound-healing activity using excision and incision wound models in rats. Group I, the control group, was treated with ointment base. Group II, which served as a reference standard, was treated with moist exposed burn ointment (MEBO). Group III was treated with RDEE. The parameters observed were percentage of wound contraction, epithelialization period, tensile strength, histopathological studies, microvessel density (MVD), and the expression of vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1). The group treated with RDEE showed significantly better wound contraction and better skin-breaking strength as compared with the control group. The results of histopathological examination, MVD, and the expression levels of growth factors supported the outcome of the wound models as well. The present study provided a scientific rationale for the traditional use of RD in the management of wounds.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Wound Healing Potential ofResina Draconis (Dracaena cochinchinensis)in Animal Models

Liu

Lin

Xiao

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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show abstract

“…10,11 This model has also been widely adopted by other investigators. 12,13 In rodents, models have been developed specifically for impaired wounds that exhibit either locally or systemically compromised and delayed healing. 14 The diabetic impaired wound models include those induced by streptozotocin injection 15 or genetically engineered for insulin dependence 16 and insulin resistance (db/db, ob/ob).…”

Section: Abstractsmentioning

confidence: 99%

“…They have since utilized the model to study the effects of cytoactive factors on wound healing . This model has also been widely adopted by other investigators …”

mentioning

confidence: 99%

Full‐thickness splinted skin wound healing models in db/db and heterozygous mice: Implications for wound healing impairment

Park

Teixeira

Raghunathan

et al. 2014

Wound Repair Regeneration

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The excisional dorsal full-thickness skin wound model with or without splinting is widely utilized in wound healing studies using diabetic or normal mice. However, the effects of splinting on dermal wound healing have not been fully characterized, and there are limited data on the direct comparison of wound parameters in the splinted model between diabetic and normal mice. We compared full-thickness excisional dermal wound healing in db/db and heterozygous mice by investigating the effects of splinting, semi-occlusive dressing, and poly(ethylene glycol) treatment. Two 8-mm full-thickness wounds were made with or without splinting in db/db and heterozygous mice. Body weights, splint maintenance, wound contraction, wound closure, and histopathological parameters including reepithelialization, wound bed collagen deposition, and inflammation were compared between groups. Our results show that silicone splint application effectively reduced wound contraction in heterozygous and db/db mice. Splinted wounds, as opposed to nonsplinted wounds, exhibited no significant differences in wound closure between heterozygous and db/db mice. Finally, polyethylene glycol and the noncontact dressing had no significant effect on wound healing in heterozygous or db/db mice. We believe these findings will help investigators in selection of the appropriate wound model and data interpretation with fully defined parameters.

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“…The silicone splinted murine wound model was developed to better approximate wound healing in humans by minimizing wound contraction which is a major difference between wound healing in mice and humans: mouse wounds heal mainly by contraction while humans by reepithelialization and granulation tissue formation . As Galiano et al published a modified dorsum excisional model in which a donut shaped silicone splint was placed around the wound, several studies that adopted this model have been published . In our experience with the splinted wound model, intact splint maintenance during the study period is challenging, especially in phenotypically normal mice that are able to destroy their splints within days and depending on applied experimental conditions.…”

mentioning

confidence: 99%

Importance of defining experimental conditions in a mouse excisional wound model

Park

Covert

Teixeira

et al. 2015

Wound Repair Regeneration

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The murine dorsum dermal excisional wound model has been widely utilized with or without splint application. However, variations in experimental methods create challenges for direct comparison of results provided in the literature and for design of new wound healing studies. Here, we investigated the effects of wound location and size, number of wounds, type of adhesive used for splint fixation on wound healing using splinted or unsplinted dorsum excisional full thickness wound models. One or two 6- or 8-mm full thickness wounds were made with or without splinting in genetically diabetic but heterozygous mice (Dock7(m) + / + Lepr(db) ). Two different adhesives: tissue adhesive and an over the counter cyanoacrylate adhesive (OTCA) "Krazy glue" were used to fix splints. Wound contraction, wound closure, and histopathological parameters including reepithelialization, collagen deposition and inflammation were compared between groups. No significant effect of wound number (1 vs. 2), side (left vs. right and cranial vs. caudal) or size on wound healing was observed. The OTCA group had a significantly higher splint success compared to the tissue adhesive group that resulted in significantly higher reepithelialization and collagen deposition in the OTCA group. Understanding the outcomes and effects of the variables will help investigators choose appropriate experimental conditions for the study purpose and interpret data.

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Effects of Adipose-derived Stromal Cells and of their Extract on Wound Healing in a Mouse Model

Cited by 51 publications

References 14 publications

Evaluation of the Wound Healing Potential ofResina Draconis (Dracaena cochinchinensis)in Animal Models

Evaluation of the Wound Healing Potential ofResina Draconis (Dracaena cochinchinensis)in Animal Models

Full‐thickness splinted skin wound healing models in db/db and heterozygous mice: Implications for wound healing impairment

Importance of defining experimental conditions in a mouse excisional wound model

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