Importance of defining experimental conditions in a mouse excisional wound model

Park, Shin Ae; Covert, Jill; Teixeira, Leandro; Motta, Monica J.; DeRemer, Sara L; Abbott, Nicholas L.; Dubielzig, Richard; Schurr, Michael J.; Isseroff, Roslyn Rivkah; McAnulty, Jonathan F.; Murphy, Christopher J.

doi:10.1111/wrr.12272

Cited by 29 publications

(21 citation statements)

References 21 publications

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“…After closure, the wound continues to contract and this provides the impression of dominant closure by contraction. Our data is consistent with prior published data that indicates a contribution of ∼50% by reepithelialization during the time period of wound closure …”

supporting

confidence: 93%

The murine excisional wound model: Contraction revisited

Chen

Mirza

Kwon

et al. 2015

Wound Repair Regeneration

142

116

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Rodent models of healing are considered limited because of the perception that rodent wounds heal by contraction while humans heal by re-epithelialization. The purpose of this report is to present evidence that simple murine excisional wounds provide a valid and reproducible wound model that heals by both contraction and re-epithelialization. Previous studies have shown that, although rodent wounds contract by up to 80%, much of this contraction occurs only after epithelial closure. To confirm these previous findings, we measured re-epithelialization and contraction in three separate mouse strains, (BALB/c, db/+ and db/db); re-epithelialization and contraction each accounted for ~40-60% of the initial closure of full thickness excisional wounds. After closure, the wound continues to contract and this provides the impression of dominant closure by contraction. In conclusion, the simple excisional rodent wound model produces a well-defined and readily identifiable wound bed over which the process of re-epithelialization is clearly measurable.

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supporting

confidence: 93%

The murine excisional wound model: Contraction revisited

Chen

Mirza

Kwon

et al. 2015

Wound Repair Regeneration

142

116

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“…This is consistent with a study on vocal fold wound healing,25 which found that astaxanthin upregulates Col1A1 expression. Wound contraction is another important mechanism in the process of wound closure, especially in rodents 26. Astaxanthin probably accelerates wound contraction during the proliferative and maturation phase by enhancing the function of myofibroblasts, the cells that are differentiated from tissue fibroblasts, which play a key role in this process.…”

Section: Discussionmentioning

confidence: 99%

Effect of astaxanthin on cutaneous wound healing

Meephansan¹,

Rungjang²,

Yingmema³

et al. 2017

CCID

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Wound healing consists of a complex series of convoluted processes which involve renewal of the skin after injury. ROS are involved in all phases of wound healing. A balance between oxidative and antioxidative forces is necessary for a favorable healing outcome. Astaxanthin, a member of the xanthophyll group, is considered a powerful antioxidant. In this study, we investigated the effect of topical astaxanthin on cutaneous wound healing. Full-thickness dermal wounds were created in 36 healthy female mice, which were divided into a control group and a group receiving 78.9 µM topical astaxanthin treatment twice daily for 15 days. Astaxanthin-treated wounds showed noticeable contraction by day 3 of treatment and complete wound closure by day 9, whereas the wounds of control mice revealed only partial epithelialization and still carried scabs. Wound healing biological markers including Col1A1 and bFGF were significantly increased in the astaxanthin-treated group since day 1. Interestingly, the oxidative stress marker iNOS showed a significantly lower expression in the study. The results indicate that astaxanthin is an effective compound for accelerating wound healing.

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“…This experimental model has been used to study the biological mechanisms involved skin wound healing 1,2 . STRO was applied topically as natural compounds with healing potential can be tested directly in wound bed 18 .…”

Section: ■ Discussionmentioning

confidence: 99%

Schinus terebinthifolius Raddi ( Aroeira) leaves oil attenuates inflammatory responses in cutaneous wound healing in mice

et al. 2017

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Schinus terebinthifolius Raddi (Aroeira) leaves oil attenuatesinflammatory responses in cutaneous wound healing in mice 1 AbstractPurpose: To investigated the inflammatory, angiogenic and fibrogenic activities of the Schinus terebinthifolius Raddi leaves oil (STRO) on wound healing. Methods:The excisional wound healing model was used to evaluate the effects of STRO. The mice were divided into two groups: Control, subjected to vehicle solution (ointment lanolin/ vaseline base), or STRO-treated group, administered topically once a day for 3, 7 and 14 days post-excision. We evaluated the macroscopic wound closure rate; the inflammation was evaluated by leukocytes accumulation and cytokine levels in the wounds. The accumulation of neutrophil and macrophages in the wounds were determined by assaying myeloperoxidase and N-acetyl-β-D-glucosaminidase activities. The levels of TNF-α, CXCL-1 and CCL-2 in wound were evaluated by ELISA assay. Angiogenesis and collagen fibers deposition were evaluated histologically. Results:We observed that macroscopic wound closure rate was improved in wounds from STRO-group than Control-group. The wounds treated with STRO promoted a reduction in leucocyte accumulation and in pro-inflammatory cytokine. Moreover, STRO treatment increased significantly the number of blood vessels and collagen fibers deposition, as compared to control group. Conclusion: Topical application of STRO display anti-inflammatory and angiogenic effects, as well as improvement in collagen replacement, suggesting a putative use of this herb for the development of phytomedicines to treat inflammatory diseases, including wound healing.

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Importance of defining experimental conditions in a mouse excisional wound model

Cited by 29 publications

References 21 publications

The murine excisional wound model: Contraction revisited

The murine excisional wound model: Contraction revisited

Effect of astaxanthin on cutaneous wound healing

Schinus terebinthifolius Raddi ( Aroeira) leaves oil attenuates inflammatory responses in cutaneous wound healing in mice

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