Effects of a herbal gel containing carvacrol and chalcones on alveolar bone resorption in rats on experimental periodontitis

Abstract

Carvacrol and dimeric chalcones are the respective bioactive components of Lippia sidoides and Myracrodruon urundeuva, popular medicinal plants of Northeastern Brazil with proven antimicrobial and antiinflammatory properties. Periodontal disease is associated with inflammation and microbiological proliferation, thus the study aimed to investigate the effect of a topical gel based on carvacrol and chalcones in the experimental periodontal disease (EPD) in rats. Animals were treated with carvacrol and/or chalcones gel, immediately after EPD induction, three times a day for 11 days. Appropriate controls were included in the study. Animals were weighed daily. They were killed on day 11, the mandibles dissected and alveolar bone loss was measured. The periodontium were examined at histopathology and the neutrophil influx into the gingiva was assayed using myeloperoxidase activity. The bacterial flora were assessed through culture of the gingival tissue. Alveolar bone loss was significantly (p < 0.05) inhibited by combined carvacrol and chalcones gel, compared with the vehicle and non-treated groups. The treatment with the combined gel reduced tissue lesion at histopathology, decreased myeloperoxidase activity in gingival tissue and inhibited the growth of oral microorganisms as well as the weight loss. Carvacrol and chalcones combination gel has a beneficial effect upon EPD in this model.

Effect of andiroba oil on periodontitis in Wistar rats

“…In several studies with similar methodology 6,12,13,18 , but with different substances testing, was found decreased inflammatory response but was not shown difference on the measurement from the cemento-enamel junction, this fact probably occurs because the short time of analysis after application of substances, which is usually after seven days. Thus, further researches are necessary to observe how much time is appropriate to manage the substances for the recovery of bone loss.…”

“…of the adult population 6 . And these rates are very high in patients with diabetes 7 , hypertension 8 or smoking 9 .…”

Evaluation of the effect of an organic extract obtained from Ipomoea alba L. on experimental periodontitis in rats

“…The sample size of this investigation was based on previous published studies. [12][13][14] General anesthesia was obtained by intra-muscular administration of ketamine hydrochloride (10 mg/kg) (Dopalen  , Agribrands Brasil Ltda., Paulínia, Brazil) and xylazine hydrochloride (10 mg/ kg) (Rompun  , Bayer S.A., São Paulo, Brazil). One of the mandibular first molars of each animal was randomly assigned to receive a cotton ligature (Corrente Algodão no.…”

Phythochemical screening and antimicrobial activity phythochemical of essential oil from Lippia gracillis

“…Essential oils exert many properties such as antinociceptive (Franco et al, 2011), anticonvulsant (Oliveira, 2009), cardiovascular effects (Menezes et al, 2010), antimicrobial activity against cariogenic bacteria (Botelho et al, 2009a) and fungal fi laments as well (Botelho et al, 2007b). Some studies point out that those plant-derived essential oils may be an effective alternative to overcome microbial resistance (Botelho et al, 2008).…”

“…This species produces an essential oil (EO) rich in thymol and carvacrol, which has a potent antimicrobial activity against fungi and bacteria. It is one of the widely used substances in Brazilian traditional medicine, in the Northeast of Brazil for skin cuts, insect bites and sore throat (Botelho et al, 2008).…”