Evaluation of the effect of an organic extract obtained from Ipomoea alba L. on experimental periodontitis in rats

Abstract

Evaluation of the effect of an organic extract obtained from Ipomoea alba L. on experimental periodontitis in rats Abstract: The aim of this study was to evaluate the effect of an organic extract obtained from Ipomoea alba L. (Convolvulaceae or OE 1493), on experimental periodontal disease in rats. Periodontitis was induced in thirty six Wistar rats: a first mandibular molar was randomly assigned to receive a ligature, whereas the contralateral molar was left unligated. Animals were randomly assigned to two groups and treated topically, three times a day, for 11 days, as follows: Control Group -vehicle-treated (n = 18), and Test Group -OE 1493-treated (n = 18). The rats were sacrificed on the 12 th day. Morphometrical measurements from the cementoenamel junction to the bone crest were performed to determine alveolar bone loss, using standardized photographs. Single-and multi-dose acute toxicity assays were carried out after OE 1493 treatment. Morphometrical analysis demonstrated that topically-administered OE 1493 showed no effect on reducing bone loss when compared with the control group (p > 0.05). In addition, OE 1493 did not present toxicity. Within the limits of this investigation, it may be concluded that OE 1493 did not show any positive influence on the progression of ligature-induced periodontitis in rats, when administered according to the regimen used in the present study.

Analysis of Brazilian Plant Extracts as Potential Source of Antioxidant Natural Products Using Bench-Top Assays

“…The evaluation of the free radical scavenging analyses were made by the establishment of scores as strong (++++), very good (+++), good (++), weak (+) and absence of free radical scavenge activity (0). 16 The following reagents were used in the chemical analysis: 11 (C) Dragendorff 's reagent, (D) 5% KOH diluted in ethanol for checking the presence of anthraquinones; (E) Kedde's reagent.…”

“…7 Our group aimed at the chemical screening of 895 organic and aqueous extracts obtained from plants found in the Amazon rain forest that have been preciously screened for their biological, [8][9][10] pharmacological and toxicological activities. [11][12][13][14][15][16] The extracts were tested for their free radical scavenging and antioxidant activities, as well as screened for the presence of alkaloids, anthraquinones and cardenolides, some of the most active classes of phytochemicals. Table 1; all vouchers are deposited at the Herbarium UNIP.…”

Resveratrol Decreases Periodontal Breakdown and Modulates Local Levels of Cytokines During Periodontitis in Rats

“…In this context, many plants or their active components used in traditional medicine might be useful for the treatment of inflammatory conditions. Amid the growing interest in plant‐derived drugs, studies have focused on the use of natural products to modulate immune‐inflammatory diseases, such as periodontitis 15‐18 …”

Synergistic effect of photodynamic therapy and alendronate on alveolar bone loss in rats with ligature‐induced periodontitis

“…Alveolar bone loss was determined on the buccal surface of the mandibular right first molar by measuring the distance from the alveolar bone crest to the cemento‐enamel junction. Measurements were made along the axis of each root in 3 regions of the first molar (the 3 roots) (Figure ) . Total alveolar bone loss was obtained from mean alveolar bone crest measurements quantified using image analysis software, ImageJ ® version 1.46 (National Institute of Health, Maryland, USA).…”