Effects of 60Co γ radiation on thylakoid membrane functions in Anacystis nidulans

Agarwal, Renu; Rane, S. S.; Sainis, Jayashree Krishna

doi:10.1016/j.jphotobiol.2008.01.006

Cited by 37 publications

(22 citation statements)

References 30 publications

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“…Therefore the percentage of C16 and C18 decreased due to the elongation process. Gamma ray radiation can cause oxidative stress in the cell affect the enzyme activity in the cell (Agarwal et al, 2008). These results were supported by the results on Desmodesmus sp., which the C16 and C18 fatty acids were decreased due to the formation of long chain fatty acids ).…”

Section: Gamma 60supporting

confidence: 69%

Efect of Gamma 60Co Irradiation on The Growth, Lipid Content and Fatty Acid Composition of Botryococcus sp. Microalgae

et al. 2017

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Section: Gamma 60supporting

confidence: 69%

Efect of Gamma 60Co Irradiation on The Growth, Lipid Content and Fatty Acid Composition of Botryococcus sp. Microalgae

et al. 2017

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“…Previously, the resistance and response of algea or cyanobacteria to radiation has been mainly investigated by morphological and physiological analysis (Kraus ; Agarwal et al. ; Singh et al. , ), but the molecular mechanisms remain to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the cellular and molecular response of Arthrospira strain PCC 8005 to high acute doses of gamma rays was investigated using transcriptomics and proteomics. Previously, the resistance and response of algea or cyanobacteria to radiation has been mainly investigated by morphological and physiological analysis (Kraus 1969;Agarwal et al 2008;Singh et al 2010aSingh et al , 2013, but the molecular mechanisms remain to be elucidated. To our knowledge, this is the first study that investigates the tolerance of the edible cyanobacterium Arthrospira to IR, at molecular level.…”

Section: Introductionmentioning

confidence: 99%

Molecular investigation of the radiation resistance of edible cyanobacterium Arthrospira sp. PCC 8005

et al. 2015

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The aim of this work was to characterize in detail the response of Arthrospira to ionizing radiation, to better understand its radiation resistance capacity. Live cells of Arthrospira sp. PCC 8005 were irradiated with 60Co gamma rays. This study is the first, showing that Arthrospira is highly tolerant to gamma rays, and can survive at least 6400 Gy (dose rate of 527 Gy h−1), which identified Arthrospira sp. PCC 8005 as a radiation resistant bacterium. Biochemical, including proteomic and transcriptomic, analysis after irradiation with 3200 or 5000 Gy showed a decline in photosystem II quantum yield, reduced carbon fixation, and reduced pigment, lipid, and secondary metabolite synthesis. Transcription of photo-sensing and signaling pathways, and thiol-based antioxidant systems was induced. Transcriptomics did show significant activation of ssDNA repair systems and mobile genetic elements (MGEs) at the RNA level. Surprisingly, the cells did not induce the classical antioxidant or DNA repair systems, such superoxide dismutase (SOD) enzyme and the RecA protein. Arthrospira cells lack the catalase gene and the LexA repressor. Irradiated Arthrospira cells did induce strongly a group of conserved proteins, of which the function in radiation resistance remains to be elucidated, but which are a promising novel routes to be explored. This study revealed the radiation resistance of Arthrospira, and the molecular systems involved, paving the way for its further and better exploitation.

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“…will also affect the increase in total lipids content. The increase in biomass will increase in the enzyme-activating effect on calvin cycle that will lead to an increase in the carboxylation reaction of 3-acid phospogliserat which led to increased cell lipid synthesis metabolism lipid (Agarwal et al, 2008).…”

Section: Effect Of Gammamentioning

confidence: 99%

“…(Chaturvedi et al, 2004). The effect of Gamma rays irradiation is oxidative stress that can influence the enzyme activity in cell (Agarwal et al, 2008). Enzyme is relevant to the induction of elongation process on fatty acid and give impact in the decrease presentation of C16 and C18 (Agarwal et al, 2008).…”

Section: Effect Of Gammamentioning

confidence: 99%

Effect of gamma 60Co irradiation on the lipid content and fatty acid composition of Nannochloropsis sp. microalgae

Ermavitalini¹,

Sari²,

Prasetyo³

et al. 2017

AIP Conference Proceedings

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Abstract. Nannochloropsissp. has been identified as a promising feed stock for biodiesel production in recent years. Nannochloropsis sp. have high lipid content between 31-68 % of dry biomass weight. Mutagenesis induced by Gamma 60 Co irradiation can be utilized to alter Nannochloropsis sp. characteristic to get microalgae mutant strain that can produce a higher lipid content than the wild strain. The aim of this research was to know the effect of gamma 60 Co irradiation to the biomass, lipid content and fatty acid compotition of Nannochloropsis sp. microalgae. In this research, irradiation was done in different doses there were 0, 2, 4, 6 and 10 Gray (Gy). Measuring microalgae biomass and lipid content were done in late exponential phase at the end of each treatment. Research design used is random complete with 95 % confident level for quantitative analysis based on biomass and lipid content. Fatty acid compotition was analyzed by gas chromatography-mass spectrometry (GC-MS). Results showed that gamma irradiated gave an effect on lipid content and fatty acid profile of Nannochloropsis sp. Tukey test showed total lipid content of control micoalgae (0 Gy) was significat different with 10 Gy irradiated microalgae, but no significant different with 2,4 and 6 Gy irradiated microalgae. The highest lipid content were found in 10 Gy irradiated microalgae equal to 62.65 %. Fatty acid profile of 10 Gy irradiated Nannochloropsis sp. had 9 fatty acids while control Nannochloropsis sp. had 6 fatty acids.

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Effects of 60Co γ radiation on thylakoid membrane functions in Anacystis nidulans

Cited by 37 publications

References 30 publications

Efect of Gamma 60Co Irradiation on The Growth, Lipid Content and Fatty Acid Composition of Botryococcus sp. Microalgae

Efect of Gamma 60Co Irradiation on The Growth, Lipid Content and Fatty Acid Composition of Botryococcus sp. Microalgae

Molecular investigation of the radiation resistance of edible cyanobacterium Arthrospira sp. PCC 8005

Effect of gamma 60Co irradiation on the lipid content and fatty acid composition of Nannochloropsis sp. microalgae

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