Effectiveness of two intracanal dressings in adult Portuguese patients: a qPCR and anaerobic culture assessment

Abstract

Infected root canals contained a high bacterial load, and the chemomechanical root canal preparation reduced bacterial equivalents by 99.1% and anaerobic counts by 98.5%. Intracanal dressings were not efficient at reducing bacterial load, but the 14-day intracanal dressing with Ca(OH)2 performed significantly better than CHX, particularly in cases with apical periodontitis.

Effects of Contemporary Irrigant Activation Schemes and Subsequent Placement of an Interim Dressing on Bacterial Presence and Activity in Root Canals Associated with Asymptomatic Apical Periodontitis

“…The rDNA-based analysis showed that the inter-appointment medication did not contribute to an additional reduction of bacterial levels after CMP. Our data corroborate previous findings from rDNA-based molecular studies [23,24]. Moreover, a high prevalence of rDNA positive samples after inter-appointment medication has been previously reported [22][23][24][25].…”

“…Our data corroborate previous findings from rDNA-based molecular studies [23,24]. Moreover, a high prevalence of rDNA positive samples after inter-appointment medication has been previously reported [22][23][24][25]. An interesting finding of this study is that the rRNA-based analysis revealed the activity of bacteria detected by rDNA in S4 samples, proving it to be a useful strategy for monitoring the antibacterial effect of endodontic procedures.…”

“…The lowest bacterial rDNA levels were found at the end of the treatment, due to the cumulative effect of the first and second visit interventions, especially the chemo-mechanical preparation (CMP) and the second-visit root canal preparation. These findings reinforce the proposition that mechanical action with instruments and antimicrobial solutions are the main strategies for root canal disinfection [11][12][13][14][21][22][23][24][25].…”

“…Moreover, the depth to which the irrigating needle was inserted in the medium/wide canals studied may have been another factor contributing to the removal of bacteria from root canals [29]. However, despite the significant reduction in bacterial load after CMP, 60% of the cases were still positive for bacterial rDNA, which is in accordance with previous molecular-based studies [11][12][13][21][22][23][24]27].…”

Análise da atividade metabólica de bactérias persistentes após os procedimentos endodônticos de desinfecção: estudo molecular baseado em RNA e DNA

“…Entretanto, existem divergências entre os estudos moleculares que avaliaram a eficácia da medicação intracanal em promover redução de micro-organismos dos canais radiculares. Alguns autores relataram uma redução do número de cópias de rDNA após o uso da medicação intracanal (Paiva et al, 2013a;Provenzano et al, 2015;Nakamura et al, 2018); outros afirmaram não haver diferença entre o número de cópias de rDNA detectados após o preparo químicocirúrgico e aquele detectado após a medicação intracanal (Sakamoto et al, 2007;Blome et al, 2008;Siqueira, 2011b;Teles et al, 2014;Ferreira et al, 2015); enquanto outro autor identificou um aumento do número de cópias de rDNA após o uso da medicação intracanal (Zandi et al, 2016). Entretanto, vale ressaltar, que esses estudos moleculares baseados em rDNA apresentam uma limitação, que é a não distinção entre as células vivas e mortas Rôças, 2005;Loozen et al, 2011;Brudin et al, 2013).…”