Effectiveness of Propolis, Probiotics And Chlorhexidine on Streptococcus Mutans And Candida Albicans: An In-Vitro Study

George, Rajani Mary; Kasliwal, Akash V.

doi:10.9790/0853-1603071518

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…Propolis with a concentration of 50 µl shows 15.6 mm mean zone of inhibition for Candida albicans as compared to probiotics 12 mm and chlorhexidine 14 mm. [65] For Streptococcus mutans, mean zone of inhibition was 9.4 mm for probiotics, 14 mm for chlorhexidine, and 14.6 mm for propolis.…”

Section: Anti-inflammatory Activitymentioning

confidence: 94%

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Apitherapy and Periodontal Disease: Insights into In Vitro, In Vivo, and Clinical Studies

et al. 2022

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Periodontal diseases are caused mainly by inflammation of the gums and bones surrounding the teeth or by dysbiosis of the oral microbiome, and the Global Burden of Disease study (2019) reported that periodontal disease affects 20–50% of the global population. In recent years, more preference has been given to natural therapies compared to synthetic drugs in the treatment of periodontal disease, and several oral care products, such as toothpaste, mouthwash, and dentifrices, have been developed comprising honeybee products, such as propolis, honey, royal jelly, and purified bee venom. In this study, we systematically reviewed the literature on the treatment of periodontitis using honeybee products. A literature search was performed using various databases, including PubMed, Web of Science, ScienceDirect, Scopus, clinicaltrials.gov, and Google Scholar. A total of 31 studies were reviewed using eligibility criteria published between January 2016 and December 2021. In vitro, in vivo, and clinical studies (randomized clinical trials) were included. Based on the results of these studies, honeybee products, such as propolis and purified bee venom, were concluded to be effective and safe for use in the treatment of periodontitis mainly due to their antimicrobial and anti-inflammatory activities. However, to obtain reliable results from randomized clinical trials assessing the effectiveness of honeybee products in periodontal treatment with long-term follow-up, a broader sample size and assessment of various clinical parameters are needed.

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Section: Anti-inflammatory Activitymentioning

confidence: 94%

“…For Streptococcus mutans, the mean zone of inhibition was 9.4 mm for probiotics, 14 mm for chlorhexidine, and 14.6 mm for propolis. Compared to standard chlorhexidine and probiotics, propolis was found to be more effective in inhibiting Streptococcus mutans and Candida albicans [65].…”

Section: Antimicrobial Studiesmentioning

confidence: 95%

Apitherapy and Periodontal Disease: Insights into In Vitro, In Vivo, and Clinical Studies

et al. 2022

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“…Bioactive molecules of natural products have pharmacological activity and have been widely studied and used by the pharmaceutical industry as an alternative to traditional medicines. 1,2 The biofilm developed on the dental surface is composed of several species of micro-organisms, initially colonized by gram-positive and aerobic bacteria, but later, there is a sequential colonization of gramnegative and anaerobic micro-organisms. Thus, a constant and effective disorganization of this biofilm is necessary to prevent these oral pathologies.…”

Section: Introductionmentioning

confidence: 99%

In vitro Evaluation of Antimicrobial Activity of Brazilian Red Propolis Containing-dentifrice

Chagas¹,

Júnior²,

Dodou³

et al. 2022

J Young Pharm

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Background: This study evaluated the antimicrobial activity of brazilian red propolis (BRP) dentifrice and compare in vitro. Materials and methods: Strains of S. mutans ATCC UA159 were used in the present study. This study compared the antibacterial effects of BRP extract, BRP containingdentifrice and one antimicrobial commercial dentifrice (Parodontax) against S. mutans. For the evaluation of the antimicrobial activity the microdilution method was used in culture broth. The strain was activated by incubation at 37°C overnight in Brain Heart Infusion (BHI) culture medium, in an anaerobic jar. To the wells of the microplates were added 100μL of BHI broth, 20μL of the substances tested (experimental groups), at concentrations ranging from 100 μL/mL to 0.0488 μL/mL, and 80 μL/mL of the standardized microbial suspension. The microplates were incubated for 24 hr in a bacteriological oven at 37°C. Visual inspection of the colour changes and reading in BioTek microplate reader at 570nm was performed to determine the Minimum Bactericidal Concentration (MBC). Mean values and standard deviations were calculated. ANOVA followed by Dunnett's test was performed; p-value of less than 0.05 was considered significant. Results: BRP extract and BRP containing-dentifrice showed antimicrobial activity against S. mutans up to the concentration of 0.3906 μg/mL. The BRP extract and dentifrice showed bactericidal effect at a concentration of 1.5625, but was able to reduce microbial viability at a concentration of up to 0.3906. Parodontax dentifrice showed antimicrobial activity at all dilutions (p< 0.05), presenting a bactericidal effect in the same concentration of the extract (1.5625). It was concluded that all the groups observed had antimicrobial activity against S. mutans. There was no difference between the propolis extract and the common dentifrice. Conclusion: BRP in pure form and processed in a toothpaste shows some antimicrobial activity against S. mutans but less than a commercial toothpaste containing herbal extracts and sodium bicarbonate.

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Antifungal activity of Propolis, Fluconazole and Chlorhexidine against Oral Candida albicans – A Comparative in-vitro Study

Sharma

Sanadhya

Nagarajappa

et al. 2022

RJPT

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Objectives: To assess the in-vitro antifungal action of propolis in contrast with the standard medications, fluconazole and chlorhexidine against Oral Candida albicans. Materials and Methods: MIC (Minimum inhibitory concentration) as well as MFC (Minimum fungicidal concentration) were attained from propolis, fluconazole and chlorhexidine against oral Candida albicans and GC-MS Analysis were completed. The analysis of variance (ANOVA) and Repeated measures ANOVA tests were used for comparison of quantitative data. Results: The mean zone of inhibition showed an increasing trend with increase in concentration of all the extracts. At all concentrations mean zone of inhibition was identified to be significantly highest with chlorhexidine. The mean zone of inhibition at 25mcg/ml concentration was found to be most noteworthy for chlorhexidine followed by ethanolic and methanolic extracts of Propolis. Conclusions: Propolis has an antifungal activity against Candida albicans. The mean zone of inhibition showed an increasing trend with increase in concentration. Candida albicans remained resistant to all propolis extracts at low concentration of 5 mcg/ml.

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Effectiveness of Propolis, Probiotics And Chlorhexidine on Streptococcus Mutans And Candida Albicans: An In-Vitro Study

Cited by 3 publications

References 11 publications

Apitherapy and Periodontal Disease: Insights into In Vitro, In Vivo, and Clinical Studies

Apitherapy and Periodontal Disease: Insights into In Vitro, In Vivo, and Clinical Studies

In vitro Evaluation of Antimicrobial Activity of Brazilian Red Propolis Containing-dentifrice

Antifungal activity of Propolis, Fluconazole and Chlorhexidine against Oral Candida albicans – A Comparative in-vitro Study

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